Obtenção e caracterização de nanopartículas de quitosana e carboximetil-β-ciclodextrina contendo resveratrol
Chitosan (CS) and carboxymethyl-β-cyclodextrin (CM-β-CD) nanoparticles were obtained spontaneously by the attraction between molecules of opposite charge. CS is a biodegradable cationic polyelectrolyte capable of interacting and forming polyelectrolytic complexes with CM-β-CD, modified anionic cy...
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Complexos supramoleculares Resveratrol Calorimetria de titulação isotérmica |
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Complexos supramoleculares Resveratrol Calorimetria de titulação isotérmica Stopilha, Roberta Talita Obtenção e caracterização de nanopartículas de quitosana e carboximetil-β-ciclodextrina contendo resveratrol |
| description |
Chitosan (CS) and carboxymethyl-β-cyclodextrin (CM-β-CD) nanoparticles were obtained
spontaneously by the attraction between molecules of opposite charge. CS is a biodegradable
cationic polyelectrolyte capable of interacting and forming polyelectrolytic complexes with
CM-β-CD, modified anionic cyclodextrin. Resveratrol (RES), a polyphenol with excellent
pharmaceutical properties, was incorporated into this system and characterized. The parameter
that changed in this study was the ratio (r) between the carboxylate groups of CM-β-CD and
the amino groups of CS, with molar ratio between 0.23 and 0.45. CM-β-CD was synthesized
from β-CD and characterized by XRD, FTIR and TG / DTG that confirmed the successful
chemical modification. The obtained CM-β-CD had a degree of substitution of 5, with a molar
mass of 1446 g/mol by Maldi TOF. Through the physical-chemical characterization it was
possible to verify the formation of nanoparticles of CS/CM-β-CD, which had a spherical shape
and particle size ranging from 100 to 400 nm, while with the RES the diameter was around 160
nm. Regarding thermal analysis, it was observed that the increase in temperature did not favor
the maintenance of the complexes between CS/CM-β-CD and CS/CM-β-CD with RES. The
ITC study was carried out to assess physical-chemical parameters of intermolecular
interactions, between CS, CDs (β-CD and CM-β-CD) and RES. Regarding the affinity between
RES and the cyclodextrins, a greater complexing capacity was observed between RES and the
anionic CD, suggesting that the complexes formed are more stable, with a 2:1 stoichiometry
(CD / RES). |
| author2 |
Fonseca, José Luís Cardozo |
| author_facet |
Fonseca, José Luís Cardozo Stopilha, Roberta Talita |
| format |
doctoralThesis |
| author |
Stopilha, Roberta Talita |
| author_sort |
Stopilha, Roberta Talita |
| title |
Obtenção e caracterização de nanopartículas de quitosana e carboximetil-β-ciclodextrina contendo resveratrol |
| title_short |
Obtenção e caracterização de nanopartículas de quitosana e carboximetil-β-ciclodextrina contendo resveratrol |
| title_full |
Obtenção e caracterização de nanopartículas de quitosana e carboximetil-β-ciclodextrina contendo resveratrol |
| title_fullStr |
Obtenção e caracterização de nanopartículas de quitosana e carboximetil-β-ciclodextrina contendo resveratrol |
| title_full_unstemmed |
Obtenção e caracterização de nanopartículas de quitosana e carboximetil-β-ciclodextrina contendo resveratrol |
| title_sort |
obtenção e caracterização de nanopartículas de quitosana e carboximetil-β-ciclodextrina contendo resveratrol |
| publisher |
Universidade Federal do Rio Grande do Norte |
| publishDate |
2021 |
| url |
https://repositorio.ufrn.br/handle/123456789/32519 |
| work_keys_str_mv |
AT stopilharobertatalita obtencaoecaracterizacaodenanoparticulasdequitosanaecarboximetilbciclodextrinacontendoresveratrol |
| _version_ |
1773960948478902272 |
| spelling |
ri-123456789-325192021-05-13T17:50:23Z Obtenção e caracterização de nanopartículas de quitosana e carboximetil-β-ciclodextrina contendo resveratrol Stopilha, Roberta Talita Fonseca, José Luís Cardozo http://lattes.cnpq.br/1239598576148044 http://lattes.cnpq.br/8143800826985556 Xavier Júnior, Francisco Humberto http://lattes.cnpq.br/8704684733554389 Wanderley Neto, Alcides de Oliveira http://lattes.cnpq.br/4789528535524269 Raffin, Fernanda Nervo http://lattes.cnpq.br/1052562183676637 Streck, Letícia http://lattes.cnpq.br/8281814580153631 Pereira, Márcia Rodrigues http://lattes.cnpq.br/5623374421279476 Magalhães, Nereide Stela Santos http://lattes.cnpq.br/1063790618901917 Complexos supramoleculares Resveratrol Calorimetria de titulação isotérmica Chitosan (CS) and carboxymethyl-β-cyclodextrin (CM-β-CD) nanoparticles were obtained spontaneously by the attraction between molecules of opposite charge. CS is a biodegradable cationic polyelectrolyte capable of interacting and forming polyelectrolytic complexes with CM-β-CD, modified anionic cyclodextrin. Resveratrol (RES), a polyphenol with excellent pharmaceutical properties, was incorporated into this system and characterized. The parameter that changed in this study was the ratio (r) between the carboxylate groups of CM-β-CD and the amino groups of CS, with molar ratio between 0.23 and 0.45. CM-β-CD was synthesized from β-CD and characterized by XRD, FTIR and TG / DTG that confirmed the successful chemical modification. The obtained CM-β-CD had a degree of substitution of 5, with a molar mass of 1446 g/mol by Maldi TOF. Through the physical-chemical characterization it was possible to verify the formation of nanoparticles of CS/CM-β-CD, which had a spherical shape and particle size ranging from 100 to 400 nm, while with the RES the diameter was around 160 nm. Regarding thermal analysis, it was observed that the increase in temperature did not favor the maintenance of the complexes between CS/CM-β-CD and CS/CM-β-CD with RES. The ITC study was carried out to assess physical-chemical parameters of intermolecular interactions, between CS, CDs (β-CD and CM-β-CD) and RES. Regarding the affinity between RES and the cyclodextrins, a greater complexing capacity was observed between RES and the anionic CD, suggesting that the complexes formed are more stable, with a 2:1 stoichiometry (CD / RES). Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES Conselho Nacional de Desenvolvimento Científico e Tecnológico - CNPq Nanopartículas de quitosana (CS) e carboximetil-β-ciclodextrina (CM-β-CD) foram obtidas, espontaneamente, pela atração entre as moléculas de carga oposta. CS é um polieletrólito catiônico biodegradável capaz de interagir e formar complexos polieletrolíticos com a CM-βCD, ciclodextrina aniônica modificada. O resveratrol (RES), um polifenol com excelentes propriedades farmacêuticas, foi incorporado a esse sistema e caracterizado. O parâmetro que variou nesse estudo foi a razão (r) entre os grupos carboxilato da CM-β-CD e os grupos amino da CS, com valores de razão molar variando entre 0,23 e 0,45. A CM-β-CD foi sintetizada a partir da β-CD e caracterizada por DRX, FTIR e TG/DTG que confirmaram o sucesso da modificação química. CM-β-CD obtida apresentou grau de substituição 5, com massa molar de 1446 g/mol por Maldi TOF. Através da caracterização físico-química foi possível constatar a formação das nanopartículas de CS/CM-β-CD, que apresentaram formato esférico e tamanho de partícula variando de 100 a 400 nm, enquanto com o RES o diâmetro foi em torno de 160 nm. Em relação a análise térmica, foi observado que o aumento da temperatura não favorece a manutenção dos complexos entre CS/CM-β-CD e CS/CM-β-CD com RES. O estudo de ITC foi realizado para avaliar parâmetros físico-químicos das interações intermoleculares, entre a CS, CDs (β-CD e CM-β-CD) e RES. Em relação a afinidade entre RES e as CDs, foi observado uma maior capacidade de complexação entre RES e a CD aniônica, sugerindo que os complexos formados são mais estáveis, com estequiometria de 2:1 (CD/RES). 2021-05-13T17:49:27Z 2021-05-13T17:49:27Z 2019-12-13 doctoralThesis STOPILHA, Roberta Talita. Obtenção e caracterização de nanopartículas de quitosana e carboximetil-β ciclodextrina contendo resveratrol. 2019. 105f. Tese (Doutorado em Desenvolvimento e Inovação Tecnológica em Medicamentos) - Centro de Ciências da Saúde, Universidade Federal do Rio Grande do Norte, Natal, 2019. https://repositorio.ufrn.br/handle/123456789/32519 pt_BR Acesso Aberto application/pdf Universidade Federal do Rio Grande do Norte Brasil UFRN PROGRAMA DE PÓS-GRADUAÇÃO EM DESENVOLVIMENTO E INOVAÇÃO TECNOLÓGICA EM MEDICAMENTOS |