Recovery and purification of cellulolytic enzymes from Aspergillus fumigatus CCT 7873 using an aqueous two-phase micellar system

Purpose: In this study, an aqueous two-phase micellar system (ATPMS), formed by the non-ionic surfactant Triton X-114, was used to investigate the partitioning of cellulolytic enzymes produced by the filamentous fungus Aspergillus fumigatus CCT 7873. Methods: Performance of the ATPMS on the partitio...

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Principais autores: Santos, Everaldo Silvino dos, Oliveira Júnior, Sérgio Dantas de, Padilha, Carlos Eduardo de Araújo, Asevedo, Estefani Alves de, Macedo, Gorete Ribeiro de
Formato: article
Idioma:English
Publicado em: Annals of Microbiology
Assuntos:
Aspergillus fumigatus
Cellulases
Micelles
Liquid-liquid extraction
Downstream processing
Endereço do item:https://repositorio.ufrn.br/handle/123456789/32311
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spelling ri-123456789-323112021-05-02T11:35:31Z Recovery and purification of cellulolytic enzymes from Aspergillus fumigatus CCT 7873 using an aqueous two-phase micellar system Santos, Everaldo Silvino dos Oliveira Júnior, Sérgio Dantas de Padilha, Carlos Eduardo de Araújo Asevedo, Estefani Alves de Macedo, Gorete Ribeiro de Aspergillus fumigatus Cellulases Micelles Liquid-liquid extraction Downstream processing Purpose: In this study, an aqueous two-phase micellar system (ATPMS), formed by the non-ionic surfactant Triton X-114, was used to investigate the partitioning of cellulolytic enzymes produced by the filamentous fungus Aspergillus fumigatus CCT 7873. Methods: Performance of the ATPMS on the partitioning of CMCase (activity on carboxymethyl cellulose) and FPase (activity on filter paper) was investigated by varying the temperature (35, 40, 45, 50, 55, 60, and 65 °C), enzyme crude extract concentration (20, 40, 60, and 80% w/w), and Triton X-114 concentration (2, 4, 6, and 8% w/w) and by adding different inorganic salts (NaCl, CaCl2, MgSO4, and MnSO4) in the system. Results: An ATPMS formed with 8% (w/w) Triton X-114 and 40% (w/w) enzymatic crude extract at a system temperature of 55 °C was most favorable for partitioning the tested enzymes. Under these conditions, a purification factor for CMCase and FPase of 10.89 and 0.65 was reached, respectively. The addition of inorganic salts changed the distribution of enzymes. Of these, CaCl2 contributed to a higher distribution coefficient (50.0), whereas for FPase, the presence of MnSO4 in the system improved the purification factor to 3.94. Conclusion: The highest values obtained for the yield and purification factors demonstrate that ATPMS is an interesting option for recovering and purifying cellulolytic enzymes 2021-04-26T13:31:43Z 2021-04-26T13:31:43Z 2020-05-04 article OLIVEIRA JÚNIOR, S.D.; PADILHA, C.E.A.; ASEVEDO, E.A.; MACEDO, G.R.; SANTOS, E.S.. Recovery and purification of cellulolytic enzymes from Aspergillus fumigatus CCT 7873 using an aqueous two-phase micellar system. Annals of Microbiology, v. 70, p. 23, 2020. Disponível em https://annalsmicrobiology.biomedcentral.com/articles/10.1186/s13213-020-01573-w. Acesso em 30 mar. 2021. https://doi.org/10.1186/s13213-020-01573-w 1590-4261 1869-2044 https://repositorio.ufrn.br/handle/123456789/32311 10.1186/s13213-020-01573-w en Attribution 3.0 Brazil http://creativecommons.org/licenses/by/3.0/br/ application/pdf Annals of Microbiology
institution Repositório Institucional
collection RI - UFRN
language English
topic Aspergillus fumigatus
Cellulases
Micelles
Liquid-liquid extraction
Downstream processing
spellingShingle Aspergillus fumigatus
Cellulases
Micelles
Liquid-liquid extraction
Downstream processing
Santos, Everaldo Silvino dos
Oliveira Júnior, Sérgio Dantas de
Padilha, Carlos Eduardo de Araújo
Asevedo, Estefani Alves de
Macedo, Gorete Ribeiro de
Recovery and purification of cellulolytic enzymes from Aspergillus fumigatus CCT 7873 using an aqueous two-phase micellar system
description Purpose: In this study, an aqueous two-phase micellar system (ATPMS), formed by the non-ionic surfactant Triton X-114, was used to investigate the partitioning of cellulolytic enzymes produced by the filamentous fungus Aspergillus fumigatus CCT 7873. Methods: Performance of the ATPMS on the partitioning of CMCase (activity on carboxymethyl cellulose) and FPase (activity on filter paper) was investigated by varying the temperature (35, 40, 45, 50, 55, 60, and 65 °C), enzyme crude extract concentration (20, 40, 60, and 80% w/w), and Triton X-114 concentration (2, 4, 6, and 8% w/w) and by adding different inorganic salts (NaCl, CaCl2, MgSO4, and MnSO4) in the system. Results: An ATPMS formed with 8% (w/w) Triton X-114 and 40% (w/w) enzymatic crude extract at a system temperature of 55 °C was most favorable for partitioning the tested enzymes. Under these conditions, a purification factor for CMCase and FPase of 10.89 and 0.65 was reached, respectively. The addition of inorganic salts changed the distribution of enzymes. Of these, CaCl2 contributed to a higher distribution coefficient (50.0), whereas for FPase, the presence of MnSO4 in the system improved the purification factor to 3.94. Conclusion: The highest values obtained for the yield and purification factors demonstrate that ATPMS is an interesting option for recovering and purifying cellulolytic enzymes
format article
author Santos, Everaldo Silvino dos
Oliveira Júnior, Sérgio Dantas de
Padilha, Carlos Eduardo de Araújo
Asevedo, Estefani Alves de
Macedo, Gorete Ribeiro de
author_facet Santos, Everaldo Silvino dos
Oliveira Júnior, Sérgio Dantas de
Padilha, Carlos Eduardo de Araújo
Asevedo, Estefani Alves de
Macedo, Gorete Ribeiro de
author_sort Santos, Everaldo Silvino dos
title Recovery and purification of cellulolytic enzymes from Aspergillus fumigatus CCT 7873 using an aqueous two-phase micellar system
title_short Recovery and purification of cellulolytic enzymes from Aspergillus fumigatus CCT 7873 using an aqueous two-phase micellar system
title_full Recovery and purification of cellulolytic enzymes from Aspergillus fumigatus CCT 7873 using an aqueous two-phase micellar system
title_fullStr Recovery and purification of cellulolytic enzymes from Aspergillus fumigatus CCT 7873 using an aqueous two-phase micellar system
title_full_unstemmed Recovery and purification of cellulolytic enzymes from Aspergillus fumigatus CCT 7873 using an aqueous two-phase micellar system
title_sort recovery and purification of cellulolytic enzymes from aspergillus fumigatus cct 7873 using an aqueous two-phase micellar system
publisher Annals of Microbiology
publishDate 2021
url https://repositorio.ufrn.br/handle/123456789/32311
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