Desenvolvimento de nanoemulsão catiônica para incorporar a peçonha do escorpião Tityus serrulatus e avaliar eficácia do soro produzido a partir de imunização de camundongos
Scorpion stings are major cause of envenoming by animals in endemic countries, like Brazil. The scorpion antivenom is product that is able to neutralize and to protect sensitive people. However, its production has been subject of improvements taking in account the innovations on the immuno-adjuvant...
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| Formato: | doctoralThesis |
| Idioma: | pt_BR |
| Publicado em: |
Universidade Federal do Rio Grande do Norte
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| Endereço do item: | https://repositorio.ufrn.br/handle/123456789/31916 |
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| Resumo: | Scorpion stings are major cause of envenoming by animals in endemic countries, like
Brazil. The scorpion antivenom is product that is able to neutralize and to protect
sensitive people. However, its production has been subject of improvements taking in
account the innovations on the immuno-adjuvant production and the difficult on the
venom extraction, from captive animals, and the venom its toxicity. Scorpion venom
has a complex composition and an adjuvant for crude venom represents a challenge
that can be possibly fulfilled with drug delivery systems based on nanotechnology. In
this research, we seek for innovation developing new nanoemulsion formulations to
act as drug delivery system for Tityus serrulatus scorpion venom and to evaluate its
immunizing properties on Balb/C mice. The nanotechnology development covered
features that positively affected the adjuvant activity. We demonstrated that low energy
techniques for production of the nanoemulsions allowed long term stability to the
formulations with reduced and uniform droplet size (below 200 nm). The developed
formulations were also robust to pH and saline variation. The formulations were also
able to be covered by PEI and to maintain their stability. The formulations
demonstrated capable to nonspecifically adsorb crude venom, as showed through
FTIR spectroscopy technique as intermolecular interactions between nanoemulsions
and crude venom. Nanoemulsions venom-load also could reduce toxicity related to
venom hemolytic activity and to present low toxicity against macrophage cells (RAW
264.7). Adjuvant activity was demonstrated in comparison with aluminum hydroxide
adjuvant, which scored for nanotechnology feature of act as crude venom adjuvant
more efficiently to induce specific anti-IgG immunoglobulins using PEI-covered
nanoemulsions. |
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