Dual insecticidal effects of Adenanthera Pavonina kunitz-type inhibitor on Plodia Interpunctella is mediated by digestive enzymes inhibition and chitin-binding properties

The Indianmeal moth, Plodia interpunctella, is one of the most damaging pests of stored products. We investigated the insecticidal properties of ApKTI, a Kunitz trypsin inhibitor from Adenanthera pavonina seeds, against P. interpunctella larvae through bioassays with artificial diet. ApKTI-fed larva...

ver descrição completa

Na minha lista:

Detalhes bibliográficos
Principais autores:	Oliveira, Caio Fernando Ramalho de, Flores, Taylla Michelle de Oliveira, Cardoso, Marlon Henrique, Oshiro, Karen Garcia Nogueira, Russi, Raphael, França, Anderson Felipe Jácome de, Santos, Elizeu Antunes dos, Franco, Octávio Luiz, Oliveira, Adeliana Silva de, Migliolo, Ludovico
Formato:	article
Idioma:	English
Publicado em:	MDPI
Assuntos:	Non-competitive inhibitor Trypsin inhibitor Peritrophic membrane
Endereço do item:	https://repositorio.ufrn.br/handle/123456789/31321
Tags:	Adicionar Tag Sem tags, seja o primeiro a adicionar uma tag!

id	ri-123456789-31321
record_format	dspace
spelling	ri-123456789-313212021-01-31T08:19:58Z Dual insecticidal effects of Adenanthera Pavonina kunitz-type inhibitor on Plodia Interpunctella is mediated by digestive enzymes inhibition and chitin-binding properties Oliveira, Caio Fernando Ramalho de Flores, Taylla Michelle de Oliveira Cardoso, Marlon Henrique Oshiro, Karen Garcia Nogueira Russi, Raphael França, Anderson Felipe Jácome de Santos, Elizeu Antunes dos Franco, Octávio Luiz Oliveira, Adeliana Silva de Migliolo, Ludovico Non-competitive inhibitor Trypsin inhibitor Peritrophic membrane The Indianmeal moth, Plodia interpunctella, is one of the most damaging pests of stored products. We investigated the insecticidal properties of ApKTI, a Kunitz trypsin inhibitor from Adenanthera pavonina seeds, against P. interpunctella larvae through bioassays with artificial diet. ApKTI-fed larvae showed reduction of up to 88% on larval weight and 75% in survival. Trypsin enzymes extracted from P. interpunctella larvae were inhibited by ApKTI, which also demonstrated capacity to bind to chitin. Kinetic studies revealed a non-competitive inhibition mechanism of ApKTI for trypsin, which were further corroborated by molecular docking studies. Furthermore, we have demonstrated that ApKTI exhibits a hydrophobic pocket near the reactive site loop probably involved in chitin interactions. Taken together, these data suggested that the insecticidal activity of ApKTI for P. interpunctella larvae involves a dual and promiscuous mechanisms biding to two completely different targets. Both processes might impair the P. interpunctella larval digestive process, leading to larvae death before reaching the pupal stage. Further studies are encouraged using ApKTI as a biotechnological tool to control insect pests in field conditions 2021-01-29T12:37:28Z 2021-01-29T12:37:28Z 2019-11-28 article OLIVEIRA, Caio Fernando Ramalho de; FLORES, Taylla Michelle de Oliveira; CARDOSO, Marlon Henrique; OSHIRO, Karen Garcia Nogueira; RUSSI, Raphael; FRANÇA, Anderson Felipe Jácome de; SANTOS, Elizeu Antunes dos; FRANCO, Octávio Luiz; OLIVEIRA, Adeliana Silva de; MIGLIOLO, Ludovico. Dual insecticidal efects of Adenanthera pavonina kunitz-type inhibitor on Plodia interpunctella is mediated by digestive enzymes inhibition and chitin-binding properties. Molecules, [s. l.], v. 24, n. 23, p. 4344-4360, 28 nov. 2019. Disponível em: https://www.mdpi.com/1420-3049/24/23/4344/htm. Acesso em: 26 jan. 2020. http://dx.doi.org/10.3390/molecules24234344 1420-3049 https://repositorio.ufrn.br/handle/123456789/31321 10.3390/molecules24234344 en Attribution 3.0 Brazil http://creativecommons.org/licenses/by/3.0/br/ application/pdf MDPI
institution	Repositório Institucional
collection	RI - UFRN
language	English
topic	Non-competitive inhibitor Trypsin inhibitor Peritrophic membrane
spellingShingle	Non-competitive inhibitor Trypsin inhibitor Peritrophic membrane Oliveira, Caio Fernando Ramalho de Flores, Taylla Michelle de Oliveira Cardoso, Marlon Henrique Oshiro, Karen Garcia Nogueira Russi, Raphael França, Anderson Felipe Jácome de Santos, Elizeu Antunes dos Franco, Octávio Luiz Oliveira, Adeliana Silva de Migliolo, Ludovico Dual insecticidal effects of Adenanthera Pavonina kunitz-type inhibitor on Plodia Interpunctella is mediated by digestive enzymes inhibition and chitin-binding properties
description	The Indianmeal moth, Plodia interpunctella, is one of the most damaging pests of stored products. We investigated the insecticidal properties of ApKTI, a Kunitz trypsin inhibitor from Adenanthera pavonina seeds, against P. interpunctella larvae through bioassays with artificial diet. ApKTI-fed larvae showed reduction of up to 88% on larval weight and 75% in survival. Trypsin enzymes extracted from P. interpunctella larvae were inhibited by ApKTI, which also demonstrated capacity to bind to chitin. Kinetic studies revealed a non-competitive inhibition mechanism of ApKTI for trypsin, which were further corroborated by molecular docking studies. Furthermore, we have demonstrated that ApKTI exhibits a hydrophobic pocket near the reactive site loop probably involved in chitin interactions. Taken together, these data suggested that the insecticidal activity of ApKTI for P. interpunctella larvae involves a dual and promiscuous mechanisms biding to two completely different targets. Both processes might impair the P. interpunctella larval digestive process, leading to larvae death before reaching the pupal stage. Further studies are encouraged using ApKTI as a biotechnological tool to control insect pests in field conditions
format	article
author	Oliveira, Caio Fernando Ramalho de Flores, Taylla Michelle de Oliveira Cardoso, Marlon Henrique Oshiro, Karen Garcia Nogueira Russi, Raphael França, Anderson Felipe Jácome de Santos, Elizeu Antunes dos Franco, Octávio Luiz Oliveira, Adeliana Silva de Migliolo, Ludovico
author_facet	Oliveira, Caio Fernando Ramalho de Flores, Taylla Michelle de Oliveira Cardoso, Marlon Henrique Oshiro, Karen Garcia Nogueira Russi, Raphael França, Anderson Felipe Jácome de Santos, Elizeu Antunes dos Franco, Octávio Luiz Oliveira, Adeliana Silva de Migliolo, Ludovico
author_sort	Oliveira, Caio Fernando Ramalho de
title	Dual insecticidal effects of Adenanthera Pavonina kunitz-type inhibitor on Plodia Interpunctella is mediated by digestive enzymes inhibition and chitin-binding properties
title_short	Dual insecticidal effects of Adenanthera Pavonina kunitz-type inhibitor on Plodia Interpunctella is mediated by digestive enzymes inhibition and chitin-binding properties
title_full	Dual insecticidal effects of Adenanthera Pavonina kunitz-type inhibitor on Plodia Interpunctella is mediated by digestive enzymes inhibition and chitin-binding properties
title_fullStr	Dual insecticidal effects of Adenanthera Pavonina kunitz-type inhibitor on Plodia Interpunctella is mediated by digestive enzymes inhibition and chitin-binding properties
title_full_unstemmed	Dual insecticidal effects of Adenanthera Pavonina kunitz-type inhibitor on Plodia Interpunctella is mediated by digestive enzymes inhibition and chitin-binding properties
title_sort	dual insecticidal effects of adenanthera pavonina kunitz-type inhibitor on plodia interpunctella is mediated by digestive enzymes inhibition and chitin-binding properties
publisher	MDPI
publishDate	2021
url	https://repositorio.ufrn.br/handle/123456789/31321
work_keys_str_mv	AT oliveiracaiofernandoramalhode dualinsecticidaleffectsofadenantherapavoninakunitztypeinhibitoronplodiainterpunctellaismediatedbydigestiveenzymesinhibitionandchitinbindingproperties AT florestayllamichelledeoliveira dualinsecticidaleffectsofadenantherapavoninakunitztypeinhibitoronplodiainterpunctellaismediatedbydigestiveenzymesinhibitionandchitinbindingproperties AT cardosomarlonhenrique dualinsecticidaleffectsofadenantherapavoninakunitztypeinhibitoronplodiainterpunctellaismediatedbydigestiveenzymesinhibitionandchitinbindingproperties AT oshirokarengarcianogueira dualinsecticidaleffectsofadenantherapavoninakunitztypeinhibitoronplodiainterpunctellaismediatedbydigestiveenzymesinhibitionandchitinbindingproperties AT russiraphael dualinsecticidaleffectsofadenantherapavoninakunitztypeinhibitoronplodiainterpunctellaismediatedbydigestiveenzymesinhibitionandchitinbindingproperties AT francaandersonfelipejacomede dualinsecticidaleffectsofadenantherapavoninakunitztypeinhibitoronplodiainterpunctellaismediatedbydigestiveenzymesinhibitionandchitinbindingproperties AT santoselizeuantunesdos dualinsecticidaleffectsofadenantherapavoninakunitztypeinhibitoronplodiainterpunctellaismediatedbydigestiveenzymesinhibitionandchitinbindingproperties AT francooctavioluiz dualinsecticidaleffectsofadenantherapavoninakunitztypeinhibitoronplodiainterpunctellaismediatedbydigestiveenzymesinhibitionandchitinbindingproperties AT oliveiraadelianasilvade dualinsecticidaleffectsofadenantherapavoninakunitztypeinhibitoronplodiainterpunctellaismediatedbydigestiveenzymesinhibitionandchitinbindingproperties AT migliololudovico dualinsecticidaleffectsofadenantherapavoninakunitztypeinhibitoronplodiainterpunctellaismediatedbydigestiveenzymesinhibitionandchitinbindingproperties
_version_	1773959983631695872

Dual insecticidal effects of Adenanthera Pavonina kunitz-type inhibitor on Plodia Interpunctella is mediated by digestive enzymes inhibition and chitin-binding properties

Registros relacionados