Antitryptical, anticoagulant and hemagglutinating activities of Eucalyptus sp. seeds
Background: Plant biodiversity has great value for science being an inexhaustible source for new bioactive molecules capable of offering environmentally friendly and innovative solutions for various areas of the industry. The scientific community has increased their interest in the study of plant sp...
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ri-123456789-313202021-01-31T08:19:59Z Antitryptical, anticoagulant and hemagglutinating activities of Eucalyptus sp. seeds Santos, Yago Queiroz dos Carelli, Gabriella Silva Campos Veras, Bruno Oliveira de Batista, Virgínia Cunha França, Anderson Felipe Jácome de Silva, Márcia Vanusa da Santos, Elizeu Antunes dos Eucalyptus sp. seed tripsin inhibitor Background: Plant biodiversity has great value for science being an inexhaustible source for new bioactive molecules capable of offering environmentally friendly and innovative solutions for various areas of the industry. The scientific community has increased their interest in the study of plant species in the search of new molecules and to determine their mechanisms of action. Plant seeds are natural sources of bioactive compounds, such as carbohydrates, lipids and proteins with special focus on enzymatic inhibitors which protect them against digestive enzymes of phytopathogens and lectins that play an important role on carbohydrate signalization and metabolism during germination. The objective of the present study was to evaluate and describe the protein profile and to test the hemagglutinating, hemolytic and anticoagulant activities, as well as the antitryptic effect of extracts and fractions obtained from seeds of Eucalyptus species. Methods: The crude protein extract was obtained from the seed of Eucalyptus sp. with 0.02 M sodium phosphate buffer, at pH 6.6, and fractionated using ammonium sulfate in order to study its antitryptical properties as well as the capacity of hemagglutination and influence on hemostasis. Results: The crude extract showed a high effectiveness for trypsin inhibition. For hemagglutinating activity, the ammonium sulfate fraction 0-30% presented better activity, while no hemolytic activity was present in the obtained fractions. For anticoagulation assay, the fraction 0-30% showed better results. Conclusions: Taken together, the obtained results demonstrate the biotechnological potential of Eucalyptus sp. seeds, although further study is still necessary to better isolate as well as describe the bioactive compounds 2021-01-29T12:31:02Z 2021-01-29T12:31:02Z 2019-01-08 article SANTOS, Yago Queiroz dos; CARELLI, Gabriella Silva Campos; VERAS, Bruno Oliveira de; BATISTA, Virgínia Cunha; FRANÇA, Anderson Felipe Jácome de; SILVA, Márcia Vanusa da; SANTOS, Elizeu Antunes dos. Antitryptical, anticoagulant and hemagglutinating activities of Eucalyptus sp. seeds. F1000Research, [s. l.], v. 8, p. 28-34, 8 jan. 2019. Disponível em: https://f1000research.com/articles/8-28/v1. Acesso em: 26 jan. 2021. http://dx.doi.org/10.12688/f1000research.17080.1 2046-1402 https://repositorio.ufrn.br/handle/123456789/31320 10.12688/f1000research.17080.1 en Attribution 3.0 Brazil http://creativecommons.org/licenses/by/3.0/br/ application/pdf F1000Research |
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Eucalyptus sp. seed tripsin inhibitor |
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Eucalyptus sp. seed tripsin inhibitor Santos, Yago Queiroz dos Carelli, Gabriella Silva Campos Veras, Bruno Oliveira de Batista, Virgínia Cunha França, Anderson Felipe Jácome de Silva, Márcia Vanusa da Santos, Elizeu Antunes dos Antitryptical, anticoagulant and hemagglutinating activities of Eucalyptus sp. seeds |
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Background: Plant biodiversity has great value for science being an inexhaustible source for new bioactive molecules capable of offering environmentally friendly and innovative solutions for various areas of the industry. The scientific community has increased their interest in the study of plant species in the search of new molecules and to determine their mechanisms of action. Plant seeds are natural sources of bioactive compounds, such as carbohydrates, lipids and proteins with special focus on enzymatic inhibitors which protect them against digestive enzymes of phytopathogens and lectins that play an important role on carbohydrate signalization and metabolism during germination. The objective of the present study was to evaluate and describe the protein profile and to test the hemagglutinating, hemolytic and anticoagulant activities, as well as the antitryptic effect of extracts and fractions obtained from seeds of Eucalyptus species. Methods: The crude protein extract was obtained from the seed of Eucalyptus sp. with 0.02 M sodium phosphate buffer, at pH 6.6, and fractionated using ammonium sulfate in order to study its antitryptical properties as well as the capacity of hemagglutination and influence on hemostasis. Results: The crude extract showed a high effectiveness for trypsin inhibition. For hemagglutinating activity, the ammonium sulfate fraction 0-30% presented better activity, while no hemolytic activity was present in the obtained fractions. For anticoagulation assay, the fraction 0-30% showed better results. Conclusions: Taken together, the obtained results demonstrate the biotechnological potential of Eucalyptus sp. seeds, although further study is still necessary to better isolate as well as describe the bioactive compounds |
format |
article |
author |
Santos, Yago Queiroz dos Carelli, Gabriella Silva Campos Veras, Bruno Oliveira de Batista, Virgínia Cunha França, Anderson Felipe Jácome de Silva, Márcia Vanusa da Santos, Elizeu Antunes dos |
author_facet |
Santos, Yago Queiroz dos Carelli, Gabriella Silva Campos Veras, Bruno Oliveira de Batista, Virgínia Cunha França, Anderson Felipe Jácome de Silva, Márcia Vanusa da Santos, Elizeu Antunes dos |
author_sort |
Santos, Yago Queiroz dos |
title |
Antitryptical, anticoagulant and hemagglutinating activities of Eucalyptus sp. seeds |
title_short |
Antitryptical, anticoagulant and hemagglutinating activities of Eucalyptus sp. seeds |
title_full |
Antitryptical, anticoagulant and hemagglutinating activities of Eucalyptus sp. seeds |
title_fullStr |
Antitryptical, anticoagulant and hemagglutinating activities of Eucalyptus sp. seeds |
title_full_unstemmed |
Antitryptical, anticoagulant and hemagglutinating activities of Eucalyptus sp. seeds |
title_sort |
antitryptical, anticoagulant and hemagglutinating activities of eucalyptus sp. seeds |
publisher |
F1000Research |
publishDate |
2021 |
url |
https://repositorio.ufrn.br/handle/123456789/31320 |
work_keys_str_mv |
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