Avaliação do efeito cicatrizante do óleo de copaíba veiculado em sistemas SNEDDS e processos terapêuticos em modelo experimental in vivo de lesões cutâneas
Chronic wound management comprises an important aspect of medical practice and requires specific treatment for effective therapeutic treatment. Historical records show that copaiba oil is used preferentially to promote wound healing and treat inflammatory and infectious diseases. However, its low...
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| Formato: | doctoralThesis |
| Idioma: | pt_BR |
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Universidade Federal do Rio Grande do Norte
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| Endereço do item: | https://repositorio.ufrn.br/handle/123456789/31279 |
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| Resumo: | Chronic wound management comprises an important aspect of medical practice
and requires specific treatment for effective therapeutic treatment. Historical
records show that copaiba oil is used preferentially to promote wound healing
and treat inflammatory and infectious diseases. However, its low solubility in
water limits its pharmacological use. In this sense, in the present research, selfnanoemulsion drug delivery systems (SNEDDS) developed by using lowconcentration (0.5%) of copaiba oil (CO) were prepared in the presence of
Tween® 80 and aqueous (neutral or saline) medium. The encapsulation of this
bioactive oil in a SNEDDS-type system allows its slow and prolonged release with
topical application in an experimental procedure for the healing of diabetic and
non-diabetic wounds. Physical-chemical analyzes were performed to
characterize the in natura CO and also encapsulated in SNEDDS formulations.
The CO-nanoformulations were prepared without the use of co-surfactant, which
justifies its foremost importance in pharmacological applications, due to the
reduction of carrier ingredients that may cause adverse reactions. Specifically,
the SNEDDS contain copaiba oil and sunflower oil (1:1) as oil phase, variable
percentages of surfactant (Tween class) and water on neutral or saline pH. The
SNEDDS-CO systems were evaluated in therapeutic healing procedures, in
which the in vivo assays for wound healing (uninfected and infected) were
performed on Wistar rats (Rattus novergicus albinus). The animals were divided
into groups of 5 animals (n = 5), submitted to surgery to remove cutaneous flap
in the dorsal region and received daily topical treatment with SNEDDS-CO
systems during the postoperative period. Histopathological and
immunohistochemical analysis were performed after 7 and 14 days of treatment
for both groups. Histomorphometric analysis revealed a lower number of
neutrophils and lymphocytes in the treated groups (7 days), as well as increased
collagenesis, proliferation of fibroblasts and increased epithelial thickening, which
by comparison were more evident in the treated groups (14 days). In the control
groups, the delay in tissue repair was related to the presence of keratinocytes as
a function of the increased immunoreactivity for IL-1β. The highest number of
cells immunocornized with FGF-2 was associated with the improvement of
collagenase and the tissue repair process. In addition, an in vivo comparative
approach of therapeutic procedures was performed in an experimental model of
cutaneous lesions, in which the effect of low intensity laser therapy (LILT) on
angiogenesis and immunoexpression of matrix metalloproteinase-2 was
evaluated in the absence and/or presence of SNEDDS-CO systems. |
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