Commercial fucoidans from Fucus vesiculosus can be grouped into antiadipogenic and adipogenic agents

Fucus vesiculosus is a brown seaweed used in the treatment of obesity. This seaweed synthesizes various bioactive molecules, one of them being a sulfated polysaccharide known as fucoidan (FF). This polymer can easily be found commercially, and has antiadipogenic and lipolytic activity. Using differe...

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Principais autores: Oliveira, Ruth Medeiros, Câmara, Rafael Barros Gomes da, Monte, Jessyka Fernanda Santiago, Viana, Rony Lucas Silva, Melo, Karoline Rachel Teodosio, Queiroz, Moacir Fernandes, Filgueira, Luciana Guimarães Alves, Oyama, Lila Missae, Rocha, Hugo Alexandre de Oliveira
Formato: article
Idioma:English
Publicado em: MDPI
Assuntos:
3T3-L1 cells
Fucan
Lipolytic
Obesity
Brown seaweed
Endereço do item:https://repositorio.ufrn.br/handle/123456789/31193
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spelling ri-123456789-311932021-11-09T19:18:38Z Commercial fucoidans from Fucus vesiculosus can be grouped into antiadipogenic and adipogenic agents Oliveira, Ruth Medeiros Câmara, Rafael Barros Gomes da Monte, Jessyka Fernanda Santiago Viana, Rony Lucas Silva Melo, Karoline Rachel Teodosio Queiroz, Moacir Fernandes Filgueira, Luciana Guimarães Alves Oyama, Lila Missae Rocha, Hugo Alexandre de Oliveira 3T3-L1 cells Fucan Lipolytic Obesity Brown seaweed Fucus vesiculosus is a brown seaweed used in the treatment of obesity. This seaweed synthesizes various bioactive molecules, one of them being a sulfated polysaccharide known as fucoidan (FF). This polymer can easily be found commercially, and has antiadipogenic and lipolytic activity. Using differential precipitation with acetone, we obtained four fucoidan-rich fractions (F0.5/F0.9/F1.1/F2.0) from FF. These fractions contain different proportions of fucose:glucuronic acid:galactose:xylose:sulfate, and also showed different electrophoretic mobility and antioxidant activity. Using 3T3-L1 adipocytes, we found that all samples had lipolytic action, especially F2.0, which tripled the amount of glycerol in the cellular medium. Moreover, we observed that FF, F1.0, and F2.0 have antiadipogenic activity, as they inhibited the oil red staining by cells at 40%, 40%, and 50%, respectively. In addition, they decreased the expression of key proteins of adipogenic differentiation (C/EBPα, C/EBPβ, and PPARγ). However, F0.5 and F0.9 stimulated the oil red staining at 80% and increased the expression of these proteins. Therefore, these fucoidan fractions have an adipogenic effect. Overall, the data show that F2.0 has great potential to be used as an agent against obesity as it displays better antioxidant, lipolytic and antiadipogenic activities than the other fucoidan fractions that we tested 2021-01-05T17:20:30Z 2021-01-05T17:20:30Z 2018-06-04 article OLIVEIRA, Ruth Medeiros; CÂMARA, Rafael Barros Gomes da; MONTE, Jessyka Fernanda Santiago; VIANA, Rony Lucas Silva; MELO, Karoline Rachel Teodosio; QUEIROZ, Moacir Fernandes; FILGUEIRA, Luciana Guimarães Alves; OYAMA, Lila Missae; ROCHA, Hugo Alexandre Oliveira. Commercial fucoidans from Fucus vesiculosus can be grouped into antiadipogenic and adipogenic agents. Marine Drugs, [s. l.], v. 16, n. 6, p. 193-206, 4 jun. 2018. MDPI AG. Disponível em: https://www.mdpi.com/1660-3397/16/6/193. Acesso em: 03 ago. 2020. http://dx.doi.org/10.3390/md16060193. 1660-3397 (online) https://repositorio.ufrn.br/handle/123456789/31193 10.3390/md16060193 en Attribution 3.0 Brazil http://creativecommons.org/licenses/by/3.0/br/ application/pdf MDPI
institution Repositório Institucional
collection RI - UFRN
language English
topic 3T3-L1 cells
Fucan
Lipolytic
Obesity
Brown seaweed
spellingShingle 3T3-L1 cells
Fucan
Lipolytic
Obesity
Brown seaweed
Oliveira, Ruth Medeiros
Câmara, Rafael Barros Gomes da
Monte, Jessyka Fernanda Santiago
Viana, Rony Lucas Silva
Melo, Karoline Rachel Teodosio
Queiroz, Moacir Fernandes
Filgueira, Luciana Guimarães Alves
Oyama, Lila Missae
Rocha, Hugo Alexandre de Oliveira
Commercial fucoidans from Fucus vesiculosus can be grouped into antiadipogenic and adipogenic agents
description Fucus vesiculosus is a brown seaweed used in the treatment of obesity. This seaweed synthesizes various bioactive molecules, one of them being a sulfated polysaccharide known as fucoidan (FF). This polymer can easily be found commercially, and has antiadipogenic and lipolytic activity. Using differential precipitation with acetone, we obtained four fucoidan-rich fractions (F0.5/F0.9/F1.1/F2.0) from FF. These fractions contain different proportions of fucose:glucuronic acid:galactose:xylose:sulfate, and also showed different electrophoretic mobility and antioxidant activity. Using 3T3-L1 adipocytes, we found that all samples had lipolytic action, especially F2.0, which tripled the amount of glycerol in the cellular medium. Moreover, we observed that FF, F1.0, and F2.0 have antiadipogenic activity, as they inhibited the oil red staining by cells at 40%, 40%, and 50%, respectively. In addition, they decreased the expression of key proteins of adipogenic differentiation (C/EBPα, C/EBPβ, and PPARγ). However, F0.5 and F0.9 stimulated the oil red staining at 80% and increased the expression of these proteins. Therefore, these fucoidan fractions have an adipogenic effect. Overall, the data show that F2.0 has great potential to be used as an agent against obesity as it displays better antioxidant, lipolytic and antiadipogenic activities than the other fucoidan fractions that we tested
format article
author Oliveira, Ruth Medeiros
Câmara, Rafael Barros Gomes da
Monte, Jessyka Fernanda Santiago
Viana, Rony Lucas Silva
Melo, Karoline Rachel Teodosio
Queiroz, Moacir Fernandes
Filgueira, Luciana Guimarães Alves
Oyama, Lila Missae
Rocha, Hugo Alexandre de Oliveira
author_facet Oliveira, Ruth Medeiros
Câmara, Rafael Barros Gomes da
Monte, Jessyka Fernanda Santiago
Viana, Rony Lucas Silva
Melo, Karoline Rachel Teodosio
Queiroz, Moacir Fernandes
Filgueira, Luciana Guimarães Alves
Oyama, Lila Missae
Rocha, Hugo Alexandre de Oliveira
author_sort Oliveira, Ruth Medeiros
title Commercial fucoidans from Fucus vesiculosus can be grouped into antiadipogenic and adipogenic agents
title_short Commercial fucoidans from Fucus vesiculosus can be grouped into antiadipogenic and adipogenic agents
title_full Commercial fucoidans from Fucus vesiculosus can be grouped into antiadipogenic and adipogenic agents
title_fullStr Commercial fucoidans from Fucus vesiculosus can be grouped into antiadipogenic and adipogenic agents
title_full_unstemmed Commercial fucoidans from Fucus vesiculosus can be grouped into antiadipogenic and adipogenic agents
title_sort commercial fucoidans from fucus vesiculosus can be grouped into antiadipogenic and adipogenic agents
publisher MDPI
publishDate 2021
url https://repositorio.ufrn.br/handle/123456789/31193
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