Genotoxicity and osteogenic potential of sulfated polysaccharides from Caulerpa prolifera seaweed

Marine algae are sources of novel bioactive molecules and present a great potential for biotechnological and biomedical applications. Although green algae are the least studied type of seaweed, several of their biological activities have already been described. Here, we investigated the osteogenic p...

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Principais autores:	Chaves Filho, Gildácio Pereira, Sousa, Angélica Fernandes Gurgel de, Câmara, Rafael Barros Gomes da, Rocha, Hugo Alexandre de Oliveira, Medeiros, Silvia Regina Batistuzzo de, Moreira, Susana Margarida Gomes
Formato:	article
Idioma:	English
Publicado em:	Elsevier
Assuntos:	Bone regeneration Osteoinductive effect Green macroalgae
Endereço do item:	https://repositorio.ufrn.br/handle/123456789/31191
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spelling	ri-123456789-311912021-11-08T20:00:25Z Genotoxicity and osteogenic potential of sulfated polysaccharides from Caulerpa prolifera seaweed Chaves Filho, Gildácio Pereira Sousa, Angélica Fernandes Gurgel de Câmara, Rafael Barros Gomes da Rocha, Hugo Alexandre de Oliveira Medeiros, Silvia Regina Batistuzzo de Moreira, Susana Margarida Gomes Bone regeneration Osteoinductive effect Green macroalgae Marine algae are sources of novel bioactive molecules and present a great potential for biotechnological and biomedical applications. Although green algae are the least studied type of seaweed, several of their biological activities have already been described. Here, we investigated the osteogenic potential of Sulfated Polysaccharide (SP)- enriched samples extracted from the green seaweed Caulerpa prolifera on human mesenchymal stem cells isolated from Wharton jelly (hMSC-WJ). In addition, the potential genotoxicity of these SPs was determined by cytokinesis-block micronucleus (CBMN) assay. SP-enriched samples did not show significant cytotoxicity towards hMSCs-WJ at a concentration of up to 10 μg/mL, and after 72 h of exposure. SP enrichment also significantly increased alkaline phosphatase (ALP) activity, promoting calcium accumulation in the extracellular matrix. Among the SP-enriched samples, the CP0.5 subfraction (at 5 μg/mL) presented the most promising results. In this sample, ALP activity was increased approximately by 60%, and calcium accumulation was approximately 6-fold above the negative control, indicating high osteogenic potential. This subfraction also proved to be non-genotoxic, according to the CBMN assay, as it did not induce micronuclei. The results of this study highlight, for the first time, the potential of these SPs for the development of new therapies for bone regeneration 2021-01-05T17:11:54Z 2021-01-05T17:11:54Z 2018 article CHAVES FILHO, Gildácio Pereira; SOUSA, Angélica Fernandes Gurgel de; CÂMARA, Rafael Barros Gomes da; ROCHA, Hugo Alexandre Oliveira; MEDEIROS, Silvia Regina Batistuzzo de; MOREIRA, Susana Margarida Gomes. Genotoxicity and osteogenic potential of sulfated polysaccharides from Caulerpa prolifera seaweed. International Journal Of Biological Macromolecules, [s. l.], v. 114, p. 565-571, jul. 2018. Elsevier BV. Disponível em: https://www.sciencedirect.com/science/article/pii/S0141813017345518?via%3Dihub. Acesso em: 03 ago. 2020. http://dx.doi.org/10.1016/j.ijbiomac.2018.03.132. 0141-8130 (print) https://repositorio.ufrn.br/handle/123456789/31191 10.1016/j.ijbiomac.2018.03.132 en Attribution 3.0 Brazil http://creativecommons.org/licenses/by/3.0/br/ application/pdf Elsevier
institution	Repositório Institucional
collection	RI - UFRN
language	English
topic	Bone regeneration Osteoinductive effect Green macroalgae
spellingShingle	Bone regeneration Osteoinductive effect Green macroalgae Chaves Filho, Gildácio Pereira Sousa, Angélica Fernandes Gurgel de Câmara, Rafael Barros Gomes da Rocha, Hugo Alexandre de Oliveira Medeiros, Silvia Regina Batistuzzo de Moreira, Susana Margarida Gomes Genotoxicity and osteogenic potential of sulfated polysaccharides from Caulerpa prolifera seaweed
description	Marine algae are sources of novel bioactive molecules and present a great potential for biotechnological and biomedical applications. Although green algae are the least studied type of seaweed, several of their biological activities have already been described. Here, we investigated the osteogenic potential of Sulfated Polysaccharide (SP)- enriched samples extracted from the green seaweed Caulerpa prolifera on human mesenchymal stem cells isolated from Wharton jelly (hMSC-WJ). In addition, the potential genotoxicity of these SPs was determined by cytokinesis-block micronucleus (CBMN) assay. SP-enriched samples did not show significant cytotoxicity towards hMSCs-WJ at a concentration of up to 10 μg/mL, and after 72 h of exposure. SP enrichment also significantly increased alkaline phosphatase (ALP) activity, promoting calcium accumulation in the extracellular matrix. Among the SP-enriched samples, the CP0.5 subfraction (at 5 μg/mL) presented the most promising results. In this sample, ALP activity was increased approximately by 60%, and calcium accumulation was approximately 6-fold above the negative control, indicating high osteogenic potential. This subfraction also proved to be non-genotoxic, according to the CBMN assay, as it did not induce micronuclei. The results of this study highlight, for the first time, the potential of these SPs for the development of new therapies for bone regeneration
format	article
author	Chaves Filho, Gildácio Pereira Sousa, Angélica Fernandes Gurgel de Câmara, Rafael Barros Gomes da Rocha, Hugo Alexandre de Oliveira Medeiros, Silvia Regina Batistuzzo de Moreira, Susana Margarida Gomes
author_facet	Chaves Filho, Gildácio Pereira Sousa, Angélica Fernandes Gurgel de Câmara, Rafael Barros Gomes da Rocha, Hugo Alexandre de Oliveira Medeiros, Silvia Regina Batistuzzo de Moreira, Susana Margarida Gomes
author_sort	Chaves Filho, Gildácio Pereira
title	Genotoxicity and osteogenic potential of sulfated polysaccharides from Caulerpa prolifera seaweed
title_short	Genotoxicity and osteogenic potential of sulfated polysaccharides from Caulerpa prolifera seaweed
title_full	Genotoxicity and osteogenic potential of sulfated polysaccharides from Caulerpa prolifera seaweed
title_fullStr	Genotoxicity and osteogenic potential of sulfated polysaccharides from Caulerpa prolifera seaweed
title_full_unstemmed	Genotoxicity and osteogenic potential of sulfated polysaccharides from Caulerpa prolifera seaweed
title_sort	genotoxicity and osteogenic potential of sulfated polysaccharides from caulerpa prolifera seaweed
publisher	Elsevier
publishDate	2021
url	https://repositorio.ufrn.br/handle/123456789/31191
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Genotoxicity and osteogenic potential of sulfated polysaccharides from Caulerpa prolifera seaweed

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