A fucan of a brown seaweed and its antitumoral property on HT-29 and immunomodulatory activity in murine RAW 264.7 macrophage cell line

Polysaccharides from algae are also proper candidates with therapeutic properties and immunomodulatory and antitumor effects. The brown seaweed Lobophora variegata synthesizes different groups of anionic polysaccharides with several biological properties. Sulfated polysaccharides were obtained by de...

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Principais autores: Pinheiro, Thuane de Sousa, Santos, Marilia da S. Nascimento, Castro, Luiza Sheyla E. P. Will, Paiva, Almino Afonso de O., Alves, Luciana G., Cruz, Ana Katarina M., Nobre, Leonardo Thiago Duarte Barreto, Alves, Monique Gabriela das Chagas Faustino, Leite, Edda Lisboa
Formato: article
Idioma:English
Publicado em: Springer
Assuntos:
Sulfated polysaccharides
Antitumoral effect
Cytotoxicity
Lobophora variegata
Phaeophyceae
Apoptosis
DTH
Endereço do item:https://repositorio.ufrn.br/handle/123456789/31172
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spelling ri-123456789-311722021-01-10T07:54:09Z A fucan of a brown seaweed and its antitumoral property on HT-29 and immunomodulatory activity in murine RAW 264.7 macrophage cell line Pinheiro, Thuane de Sousa Santos, Marilia da S. Nascimento Castro, Luiza Sheyla E. P. Will Paiva, Almino Afonso de O. Alves, Luciana G. Cruz, Ana Katarina M. Nobre, Leonardo Thiago Duarte Barreto Alves, Monique Gabriela das Chagas Faustino Leite, Edda Lisboa Sulfated polysaccharides Antitumoral effect Cytotoxicity Lobophora variegata Phaeophyceae Apoptosis DTH Polysaccharides from algae are also proper candidates with therapeutic properties and immunomodulatory and antitumor effects. The brown seaweed Lobophora variegata synthesizes different groups of anionic polysaccharides with several biological properties. Sulfated polysaccharides were obtained by delipidation of seaweed, proteolysis, and fractionation with different volumes of acetone. A fraction of sulfated polysaccharides, fucans or fucoidans, was extracted with 0.8 v acetone and named L. variegata (LV). This fucan was assessed in the inflammatory process in rats, antitumor action on human colon adenocarcinoma (HT-29 cells), apoptosis, and its effect on the cell cycle. LV fraction, a galactofucan, exhibited a high ratio of total sugar/sulfate (1.5) and a very low level of proteins. This polysaccharide showed an antiinflammatory effect on two models of inflammation induced by croton oil and oxazolone in rats. LV was analyzed in cellular proliferation of HT-29. We also demonstrated the cytotoxic action against this cell line and induction in the apoptosis and decreased the cell cycle in phases S and G2/M and the accumulation of cells in the G1 phase. Our studies with LV showed a marked immunomodulatory action without and with lipopolysaccharide (LPS) on RAW 264.7 cells. Comparative studies of two fucans, LV and FV (Fucus vesiculosus), with different structures were assessed on viability in macrophages, RAW 264.7 cells. Both showed cytotoxicity in these cells. We observed high levels of nitric oxide (NO) production, interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-α) when treated at 0.25, 0.5, and 1.0 mg mL−1 of LV. Data also suggest that LV has potential antitumor effects on HT-29 and antiinflammatory and immunomodulatory effects on RAW 264.7 cells 2021-01-05T11:37:42Z 2021-01-05T11:37:42Z 2017-02-19 article PINHEIRO, Thuane de Sousa; SANTOS, Marilia da S. Nascimento; CASTRO, Luiza Sheyla E. P. Will; PAIVA, Almino Afonso de O.; ALVES, Luciana G.; CRUZ, Ana Katarina M.; NOBRE, Leonardo Thiago Duarte Barreto; ALVES, Monique Gabriela das Chagas Faustino; LEITE, Edda Lisboa. A fucan of a brown seaweed and its antitumoral property on HT-29 and immunomodulatory activity in murine RAW 264.7 macrophage cell line. Journal Of Applied Phycology, [s. l.], v. 29, n. 4, p. 2061-2075, 19 fev. 2017. Springer Science and Business Media LLC. Disponível em: https://link.springer.com/article/10.1007/s10811-017-1075-9. Acesso em: 21 set. 2020. http://dx.doi.org/10.1007/s10811-017-1075-9. 0921-8971 (print) 1573-5176 (online) https://repositorio.ufrn.br/handle/123456789/31172 10.1007/s10811-017-1075-9 en Attribution 3.0 Brazil http://creativecommons.org/licenses/by/3.0/br/ application/pdf Springer
institution Repositório Institucional
collection RI - UFRN
language English
topic Sulfated polysaccharides
Antitumoral effect
Cytotoxicity
Lobophora variegata
Phaeophyceae
Apoptosis
DTH
spellingShingle Sulfated polysaccharides
Antitumoral effect
Cytotoxicity
Lobophora variegata
Phaeophyceae
Apoptosis
DTH
Pinheiro, Thuane de Sousa
Santos, Marilia da S. Nascimento
Castro, Luiza Sheyla E. P. Will
Paiva, Almino Afonso de O.
Alves, Luciana G.
Cruz, Ana Katarina M.
Nobre, Leonardo Thiago Duarte Barreto
Alves, Monique Gabriela das Chagas Faustino
Leite, Edda Lisboa
A fucan of a brown seaweed and its antitumoral property on HT-29 and immunomodulatory activity in murine RAW 264.7 macrophage cell line
description Polysaccharides from algae are also proper candidates with therapeutic properties and immunomodulatory and antitumor effects. The brown seaweed Lobophora variegata synthesizes different groups of anionic polysaccharides with several biological properties. Sulfated polysaccharides were obtained by delipidation of seaweed, proteolysis, and fractionation with different volumes of acetone. A fraction of sulfated polysaccharides, fucans or fucoidans, was extracted with 0.8 v acetone and named L. variegata (LV). This fucan was assessed in the inflammatory process in rats, antitumor action on human colon adenocarcinoma (HT-29 cells), apoptosis, and its effect on the cell cycle. LV fraction, a galactofucan, exhibited a high ratio of total sugar/sulfate (1.5) and a very low level of proteins. This polysaccharide showed an antiinflammatory effect on two models of inflammation induced by croton oil and oxazolone in rats. LV was analyzed in cellular proliferation of HT-29. We also demonstrated the cytotoxic action against this cell line and induction in the apoptosis and decreased the cell cycle in phases S and G2/M and the accumulation of cells in the G1 phase. Our studies with LV showed a marked immunomodulatory action without and with lipopolysaccharide (LPS) on RAW 264.7 cells. Comparative studies of two fucans, LV and FV (Fucus vesiculosus), with different structures were assessed on viability in macrophages, RAW 264.7 cells. Both showed cytotoxicity in these cells. We observed high levels of nitric oxide (NO) production, interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-α) when treated at 0.25, 0.5, and 1.0 mg mL−1 of LV. Data also suggest that LV has potential antitumor effects on HT-29 and antiinflammatory and immunomodulatory effects on RAW 264.7 cells
format article
author Pinheiro, Thuane de Sousa
Santos, Marilia da S. Nascimento
Castro, Luiza Sheyla E. P. Will
Paiva, Almino Afonso de O.
Alves, Luciana G.
Cruz, Ana Katarina M.
Nobre, Leonardo Thiago Duarte Barreto
Alves, Monique Gabriela das Chagas Faustino
Leite, Edda Lisboa
author_facet Pinheiro, Thuane de Sousa
Santos, Marilia da S. Nascimento
Castro, Luiza Sheyla E. P. Will
Paiva, Almino Afonso de O.
Alves, Luciana G.
Cruz, Ana Katarina M.
Nobre, Leonardo Thiago Duarte Barreto
Alves, Monique Gabriela das Chagas Faustino
Leite, Edda Lisboa
author_sort Pinheiro, Thuane de Sousa
title A fucan of a brown seaweed and its antitumoral property on HT-29 and immunomodulatory activity in murine RAW 264.7 macrophage cell line
title_short A fucan of a brown seaweed and its antitumoral property on HT-29 and immunomodulatory activity in murine RAW 264.7 macrophage cell line
title_full A fucan of a brown seaweed and its antitumoral property on HT-29 and immunomodulatory activity in murine RAW 264.7 macrophage cell line
title_fullStr A fucan of a brown seaweed and its antitumoral property on HT-29 and immunomodulatory activity in murine RAW 264.7 macrophage cell line
title_full_unstemmed A fucan of a brown seaweed and its antitumoral property on HT-29 and immunomodulatory activity in murine RAW 264.7 macrophage cell line
title_sort fucan of a brown seaweed and its antitumoral property on ht-29 and immunomodulatory activity in murine raw 264.7 macrophage cell line
publisher Springer
publishDate 2021
url https://repositorio.ufrn.br/handle/123456789/31172
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