A low-molecular-weight galactofucan from the seaweed, Spatoglossum schröederi, binds fibronectin and inhibits capillary-like tube formation in vitro

A low-molecular-weight (LMW) heterofucan (designated fucan B) was obtained from the brown seaweed, Spatoglossum schröederi, and its activity as an inhibitor of capillary-like tube formation by endothelial cells (ECs) was analyzed. Chemical, infrared and electrophoretic analyses confirmed the identit...

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Principais autores: Menezes, Maira Maria, Nobre, Leonardo Thiago Duarte Barreto, Rossi, Gustavo Rodrigues, Almeida-Lima, Jailma, Melo-Silveira, Raniere Fagundes, Franco, Celia Regina Cavichiolo, Trindade, Edvaldo Silva, Nader, Helena Bonciani, Rocha, Hugo Alexandre de Oliveira
Formato: article
Idioma:English
Publicado em: Elsevier
Assuntos:
Fucoidan
Brown seaweed, endothelial cells
Anti-angiogenic
Endereço do item:https://repositorio.ufrn.br/handle/123456789/31159
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spelling ri-123456789-311592021-01-03T08:24:16Z A low-molecular-weight galactofucan from the seaweed, Spatoglossum schröederi, binds fibronectin and inhibits capillary-like tube formation in vitro Menezes, Maira Maria Nobre, Leonardo Thiago Duarte Barreto Rossi, Gustavo Rodrigues Almeida-Lima, Jailma Melo-Silveira, Raniere Fagundes Franco, Celia Regina Cavichiolo Trindade, Edvaldo Silva Nader, Helena Bonciani Rocha, Hugo Alexandre de Oliveira Fucoidan Brown seaweed, endothelial cells Anti-angiogenic A low-molecular-weight (LMW) heterofucan (designated fucan B) was obtained from the brown seaweed, Spatoglossum schröederi, and its activity as an inhibitor of capillary-like tube formation by endothelial cells (ECs) was analyzed. Chemical, infrared and electrophoretic analyses confirmed the identity of fucan B. In contrast to other LMW fucans, fucan B (0.012–0.1 mg/mL) inhibited ECs capillary-like tube formation in a concentrationdependent manner. In addition, fucan B (0.01–0.05 mg/mL) did not affect ECs proliferation. Fucan B also inhibited ECs migration on a fibronectin-coated surface, but not on laminin- or collagen-coated surfaces. Biotinylated fucan B was used as a probe to identify its localization. Confocal microscopy experiments revealed that biotinylated fucan did not bind to the cell surface, but rather only to fibronectin. Our findings suggest that fucan B inhibits ECs capillary-like tube formation and migration by binding directly to fibronectin and blocking fibronectin sites recognized by cell surface ligands. However, further studies are needed to evaluate the in vivo effects of fucan B 2020-12-30T13:37:13Z 2020-12-30T13:37:13Z 2018-05 article MENEZES, Maira Maria; NOBRE, Leonardo Thiago Duarte Barreto; ROSSI, Gustavo Rodrigues; ALMEIDA-LIMA, Jailma; MELO-SILVEIRA, Raniere Fagundes; FRANCO, Celia Regina Cavichiolo; TRINDADE, Edvaldo Silva; NADER, Helena Bonciani; ROCHA, Hugo Alexandre de Oliveira. A low-molecular-weight galactofucan from the seaweed, Spatoglossum schröederi, binds fibronectin and inhibits capillary-like tube formation in vitro. International Journal Of Biological Macromolecules, [s. l.], v. 111, p. 1067-1075, maio 2018. Elsevier BV. Disponível em: https://www.sciencedirect.com/science/article/pii/S0141813017333792?via%3Dihub. Acesso em: 18 set. 2020.http://dx.doi.org/10.1016/j.ijbiomac.2018.01.119. 0141-8130 (print) https://repositorio.ufrn.br/handle/123456789/31159 10.1016/j.ijbiomac.2018.01.119 en Attribution 3.0 Brazil http://creativecommons.org/licenses/by/3.0/br/ application/pdf Elsevier
institution Repositório Institucional
collection RI - UFRN
language English
topic Fucoidan
Brown seaweed, endothelial cells
Anti-angiogenic
spellingShingle Fucoidan
Brown seaweed, endothelial cells
Anti-angiogenic
Menezes, Maira Maria
Nobre, Leonardo Thiago Duarte Barreto
Rossi, Gustavo Rodrigues
Almeida-Lima, Jailma
Melo-Silveira, Raniere Fagundes
Franco, Celia Regina Cavichiolo
Trindade, Edvaldo Silva
Nader, Helena Bonciani
Rocha, Hugo Alexandre de Oliveira
A low-molecular-weight galactofucan from the seaweed, Spatoglossum schröederi, binds fibronectin and inhibits capillary-like tube formation in vitro
description A low-molecular-weight (LMW) heterofucan (designated fucan B) was obtained from the brown seaweed, Spatoglossum schröederi, and its activity as an inhibitor of capillary-like tube formation by endothelial cells (ECs) was analyzed. Chemical, infrared and electrophoretic analyses confirmed the identity of fucan B. In contrast to other LMW fucans, fucan B (0.012–0.1 mg/mL) inhibited ECs capillary-like tube formation in a concentrationdependent manner. In addition, fucan B (0.01–0.05 mg/mL) did not affect ECs proliferation. Fucan B also inhibited ECs migration on a fibronectin-coated surface, but not on laminin- or collagen-coated surfaces. Biotinylated fucan B was used as a probe to identify its localization. Confocal microscopy experiments revealed that biotinylated fucan did not bind to the cell surface, but rather only to fibronectin. Our findings suggest that fucan B inhibits ECs capillary-like tube formation and migration by binding directly to fibronectin and blocking fibronectin sites recognized by cell surface ligands. However, further studies are needed to evaluate the in vivo effects of fucan B
format article
author Menezes, Maira Maria
Nobre, Leonardo Thiago Duarte Barreto
Rossi, Gustavo Rodrigues
Almeida-Lima, Jailma
Melo-Silveira, Raniere Fagundes
Franco, Celia Regina Cavichiolo
Trindade, Edvaldo Silva
Nader, Helena Bonciani
Rocha, Hugo Alexandre de Oliveira
author_facet Menezes, Maira Maria
Nobre, Leonardo Thiago Duarte Barreto
Rossi, Gustavo Rodrigues
Almeida-Lima, Jailma
Melo-Silveira, Raniere Fagundes
Franco, Celia Regina Cavichiolo
Trindade, Edvaldo Silva
Nader, Helena Bonciani
Rocha, Hugo Alexandre de Oliveira
author_sort Menezes, Maira Maria
title A low-molecular-weight galactofucan from the seaweed, Spatoglossum schröederi, binds fibronectin and inhibits capillary-like tube formation in vitro
title_short A low-molecular-weight galactofucan from the seaweed, Spatoglossum schröederi, binds fibronectin and inhibits capillary-like tube formation in vitro
title_full A low-molecular-weight galactofucan from the seaweed, Spatoglossum schröederi, binds fibronectin and inhibits capillary-like tube formation in vitro
title_fullStr A low-molecular-weight galactofucan from the seaweed, Spatoglossum schröederi, binds fibronectin and inhibits capillary-like tube formation in vitro
title_full_unstemmed A low-molecular-weight galactofucan from the seaweed, Spatoglossum schröederi, binds fibronectin and inhibits capillary-like tube formation in vitro
title_sort low-molecular-weight galactofucan from the seaweed, spatoglossum schröederi, binds fibronectin and inhibits capillary-like tube formation in vitro
publisher Elsevier
publishDate 2020
url https://repositorio.ufrn.br/handle/123456789/31159
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