Trypanosoma cruzi III causing the indeterminate form of Chagas disease in a semi-arid region of Brazil

Objective: Trypanosoma cruzi is subdivided into six discrete typing units (DTUs), TcI–TcVI. The precise identification of each can contribute to tracking wild DTUs that invade the domiciliary environment. Methods: Twenty T. cruzi stocks isolated from 16 chagasic patients, two Panstrongylus lutzi, on...

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Principais autores: Martins, Kiev, Andrade, Cléber de Mesquita, Barbosa-Silva, Andressa Noronha, Nascimento, Gerson Barbosa do, Chiari, Egler, Galvão, Lúcia Maria da Cunha, Câmara, Antonia Cláudia Jácome da
Formato: article
Idioma:English
Publicado em: Elsevier
Assuntos:
Trypanosoma cruzi
Discrete typing units
Chronic chagasic patients
Clinical forms
Genetic diversity
Endereço do item:https://repositorio.ufrn.br/handle/123456789/31097
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spelling ri-123456789-310972020-12-27T08:10:44Z Trypanosoma cruzi III causing the indeterminate form of Chagas disease in a semi-arid region of Brazil Martins, Kiev Andrade, Cléber de Mesquita Barbosa-Silva, Andressa Noronha Nascimento, Gerson Barbosa do Chiari, Egler Galvão, Lúcia Maria da Cunha Câmara, Antonia Cláudia Jácome da Trypanosoma cruzi Discrete typing units Chronic chagasic patients Clinical forms Genetic diversity Objective: Trypanosoma cruzi is subdivided into six discrete typing units (DTUs), TcI–TcVI. The precise identification of each can contribute to tracking wild DTUs that invade the domiciliary environment. Methods: Twenty T. cruzi stocks isolated from 16 chagasic patients, two Panstrongylus lutzi, one Galea spixii, and one Euphractus sexcinctus, from different localities in the State of Rio Grande do Norte, Brazil, were characterized by genotyping the 30 region of the 24Sa rRNA gene, the mitochondrial cytochrome oxidase subunit 2 gene, and the spliced leader intergenic region. Results: TcIII was identified in 18.7% (3/16) of patients from different municipalities, as well as in P. lutzi, G. spixii, and E. sexcinctus, indicating the connection between the sylvatic and domestic cycles in this Brazilian semi-arid region. TcI and TcII were also detected, in 37.5% (6/16) and 43.8% (7/16) of patients, respectively. These DTUs were associated with cardiac, digestive, and indeterminate clinical forms, while TcIII was identified only in patients with the indeterminate form. Conclusions: The occurrence of these DTUs reveals important phylogenetic diversity in T. cruzi isolates from humans. TcIII is reported for the first time in northeastern Brazil. These findings appear to indicate an overlap between the sylvatic and domestic transmission cycles of the parasite in this region. 2020-12-22T11:12:48Z 2020-12-22T11:12:48Z 2015-08 article MARTINS, Kiev; ANDRADE, Cléber de Mesquita; BARBOSA-SILVA, Andressa Noronha; NASCIMENTO, Gerson Barbosa do; CHIARI, Egler; GALVÃO, Lúcia Maria da Cunha; CÂMARA, Antonia Cláudia Jácome da. Trypanosoma cruzi III causing the indeterminate form of Chagas disease in a semi-arid region of Brazil. International Journal Of Infectious Diseases, [s. l.], v. 39, p. 68-75, out. 2015. Elsevier BV. http://dx.doi.org/10.1016/j.ijid.2015.08.012. Disponível em: https://www.ijidonline.com/article/S1201-9712(15)00207-6/fulltext. Acesso em: 09 nov. 2020. 1201-9712 (print) https://repositorio.ufrn.br/handle/123456789/31097 en Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ application/pdf Elsevier
institution Repositório Institucional
collection RI - UFRN
language English
topic Trypanosoma cruzi
Discrete typing units
Chronic chagasic patients
Clinical forms
Genetic diversity
spellingShingle Trypanosoma cruzi
Discrete typing units
Chronic chagasic patients
Clinical forms
Genetic diversity
Martins, Kiev
Andrade, Cléber de Mesquita
Barbosa-Silva, Andressa Noronha
Nascimento, Gerson Barbosa do
Chiari, Egler
Galvão, Lúcia Maria da Cunha
Câmara, Antonia Cláudia Jácome da
Trypanosoma cruzi III causing the indeterminate form of Chagas disease in a semi-arid region of Brazil
description Objective: Trypanosoma cruzi is subdivided into six discrete typing units (DTUs), TcI–TcVI. The precise identification of each can contribute to tracking wild DTUs that invade the domiciliary environment. Methods: Twenty T. cruzi stocks isolated from 16 chagasic patients, two Panstrongylus lutzi, one Galea spixii, and one Euphractus sexcinctus, from different localities in the State of Rio Grande do Norte, Brazil, were characterized by genotyping the 30 region of the 24Sa rRNA gene, the mitochondrial cytochrome oxidase subunit 2 gene, and the spliced leader intergenic region. Results: TcIII was identified in 18.7% (3/16) of patients from different municipalities, as well as in P. lutzi, G. spixii, and E. sexcinctus, indicating the connection between the sylvatic and domestic cycles in this Brazilian semi-arid region. TcI and TcII were also detected, in 37.5% (6/16) and 43.8% (7/16) of patients, respectively. These DTUs were associated with cardiac, digestive, and indeterminate clinical forms, while TcIII was identified only in patients with the indeterminate form. Conclusions: The occurrence of these DTUs reveals important phylogenetic diversity in T. cruzi isolates from humans. TcIII is reported for the first time in northeastern Brazil. These findings appear to indicate an overlap between the sylvatic and domestic transmission cycles of the parasite in this region.
format article
author Martins, Kiev
Andrade, Cléber de Mesquita
Barbosa-Silva, Andressa Noronha
Nascimento, Gerson Barbosa do
Chiari, Egler
Galvão, Lúcia Maria da Cunha
Câmara, Antonia Cláudia Jácome da
author_facet Martins, Kiev
Andrade, Cléber de Mesquita
Barbosa-Silva, Andressa Noronha
Nascimento, Gerson Barbosa do
Chiari, Egler
Galvão, Lúcia Maria da Cunha
Câmara, Antonia Cláudia Jácome da
author_sort Martins, Kiev
title Trypanosoma cruzi III causing the indeterminate form of Chagas disease in a semi-arid region of Brazil
title_short Trypanosoma cruzi III causing the indeterminate form of Chagas disease in a semi-arid region of Brazil
title_full Trypanosoma cruzi III causing the indeterminate form of Chagas disease in a semi-arid region of Brazil
title_fullStr Trypanosoma cruzi III causing the indeterminate form of Chagas disease in a semi-arid region of Brazil
title_full_unstemmed Trypanosoma cruzi III causing the indeterminate form of Chagas disease in a semi-arid region of Brazil
title_sort trypanosoma cruzi iii causing the indeterminate form of chagas disease in a semi-arid region of brazil
publisher Elsevier
publishDate 2020
url https://repositorio.ufrn.br/handle/123456789/31097
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