Presence of metalloproteinases 2 and 9 and 8-OHdG in the fibrotic process in skeletal muscle of Mdx mice

Inflammation and oxidative stress occurs in muscle of Duchenne muscular dystrophy (DMD). The relationship between a panel of biomarkers and the DMD outcome is necessary to indicate of disease progression and response to rehabilitation programs. The aim was to analyze the connective tissue of muscle...

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Principais autores: Souza, Lidiane Begalli de, Maziero, Carla, Lazzarin, Mariana Cruz, Quintana, Hananiah Tardivo, Tomé, Tabata de Carvalho, Baptista, Vivianne Izabelle de Araújo, Oliveira, Flavia de
Formato: article
Idioma:English
Publicado em: Elsevier
Assuntos:
Duchenne muscular dystrophy
Skeletal muscle
MMP-2
MMP-9
8-OHdG
Endereço do item:https://repositorio.ufrn.br/handle/123456789/31073
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spelling ri-123456789-310732022-12-06T20:42:55Z Presence of metalloproteinases 2 and 9 and 8-OHdG in the fibrotic process in skeletal muscle of Mdx mice Souza, Lidiane Begalli de Maziero, Carla Lazzarin, Mariana Cruz Quintana, Hananiah Tardivo Tomé, Tabata de Carvalho Baptista, Vivianne Izabelle de Araújo Oliveira, Flavia de Duchenne muscular dystrophy Skeletal muscle MMP-2 MMP-9 8-OHdG Inflammation and oxidative stress occurs in muscle of Duchenne muscular dystrophy (DMD). The relationship between a panel of biomarkers and the DMD outcome is necessary to indicate of disease progression and response to rehabilitation programs. The aim was to analyze the connective tissue of muscle of Mdx mice and immunoexpression of MMP-2, MMP-9, and 8-OHdG, which signalizes oxidative stress related to DNA damage. Biceps brachii of male C57BL/10 and C57BL/10-Dmdmdx mice was submitted to Hematoxylin-Eosin, Sirius red and immunohistochemistry (MMP-2, MMP-9 and 8-OHdG) analysis. Mdx showed focal lesions with intense inflammation and fibrosis related to immunoexpression of MMP-2 and MMP-9, proving the hypothesis that these MMPs are linked to muscular tissue degeneration, which can be regenerated by their inhibition, improving the treatment of DMD carriers. Histopathological findings related to centralized nuclei increase were related to higher 8-OHdG immunomarked nuclei in Mdx, which signalizes oxidative stress associated with DNA damage provoked by DMD. Such result shows that the evaluation of 8-OHdG during the evolution of the disease could be a method to evaluate DMD disease progression 2020-12-21T13:14:33Z 2020-12-21T13:14:33Z 2020-01 article SOUZA, Lidiane Begalli de; MAZIERO, Carla; LAZZARIN, Mariana Cruz; QUINTANA, Hananiah Tardivo; TOMÉ, Tabata de Carvalho; BAPTISTA, Vivianne Izabelle de Araújo; OLIVEIRA, Flavia de. Presence of metalloproteinases 2 and 9 and 8-OHdG in the fibrotic process in skeletal muscle of Mdx mice. Acta Histochemica, [s. l.], v. 122, n. 1, p. 1-5, jan. 2020. Disponível em: https://www.sciencedirect.com/science/article/pii/S0065128119301801?via%3Dihub. Acesso em: 03 nov. 2020. http://dx.doi.org/10.1016/j.acthis.2019.151458 0065-1281 https://repositorio.ufrn.br/handle/123456789/31073 10.1016/j.acthis.2019.151458 en Attribution 3.0 Brazil http://creativecommons.org/licenses/by/3.0/br/ Elsevier
institution Repositório Institucional
collection RI - UFRN
language English
topic Duchenne muscular dystrophy
Skeletal muscle
MMP-2
MMP-9
8-OHdG
spellingShingle Duchenne muscular dystrophy
Skeletal muscle
MMP-2
MMP-9
8-OHdG
Souza, Lidiane Begalli de
Maziero, Carla
Lazzarin, Mariana Cruz
Quintana, Hananiah Tardivo
Tomé, Tabata de Carvalho
Baptista, Vivianne Izabelle de Araújo
Oliveira, Flavia de
Presence of metalloproteinases 2 and 9 and 8-OHdG in the fibrotic process in skeletal muscle of Mdx mice
description Inflammation and oxidative stress occurs in muscle of Duchenne muscular dystrophy (DMD). The relationship between a panel of biomarkers and the DMD outcome is necessary to indicate of disease progression and response to rehabilitation programs. The aim was to analyze the connective tissue of muscle of Mdx mice and immunoexpression of MMP-2, MMP-9, and 8-OHdG, which signalizes oxidative stress related to DNA damage. Biceps brachii of male C57BL/10 and C57BL/10-Dmdmdx mice was submitted to Hematoxylin-Eosin, Sirius red and immunohistochemistry (MMP-2, MMP-9 and 8-OHdG) analysis. Mdx showed focal lesions with intense inflammation and fibrosis related to immunoexpression of MMP-2 and MMP-9, proving the hypothesis that these MMPs are linked to muscular tissue degeneration, which can be regenerated by their inhibition, improving the treatment of DMD carriers. Histopathological findings related to centralized nuclei increase were related to higher 8-OHdG immunomarked nuclei in Mdx, which signalizes oxidative stress associated with DNA damage provoked by DMD. Such result shows that the evaluation of 8-OHdG during the evolution of the disease could be a method to evaluate DMD disease progression
format article
author Souza, Lidiane Begalli de
Maziero, Carla
Lazzarin, Mariana Cruz
Quintana, Hananiah Tardivo
Tomé, Tabata de Carvalho
Baptista, Vivianne Izabelle de Araújo
Oliveira, Flavia de
author_facet Souza, Lidiane Begalli de
Maziero, Carla
Lazzarin, Mariana Cruz
Quintana, Hananiah Tardivo
Tomé, Tabata de Carvalho
Baptista, Vivianne Izabelle de Araújo
Oliveira, Flavia de
author_sort Souza, Lidiane Begalli de
title Presence of metalloproteinases 2 and 9 and 8-OHdG in the fibrotic process in skeletal muscle of Mdx mice
title_short Presence of metalloproteinases 2 and 9 and 8-OHdG in the fibrotic process in skeletal muscle of Mdx mice
title_full Presence of metalloproteinases 2 and 9 and 8-OHdG in the fibrotic process in skeletal muscle of Mdx mice
title_fullStr Presence of metalloproteinases 2 and 9 and 8-OHdG in the fibrotic process in skeletal muscle of Mdx mice
title_full_unstemmed Presence of metalloproteinases 2 and 9 and 8-OHdG in the fibrotic process in skeletal muscle of Mdx mice
title_sort presence of metalloproteinases 2 and 9 and 8-ohdg in the fibrotic process in skeletal muscle of mdx mice
publisher Elsevier
publishDate 2020
url https://repositorio.ufrn.br/handle/123456789/31073
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