Marcadores barcode em Spondias do Nordeste: o caso específico de S. bahiensis
Spondias bahiensisP. Carvalho, Van den Berg & M. Machado is one of the five species ofSpondiasgenus present in Brazil, mainly occurring in the Northeast of Brazil. The plant was identified for many years asSpondias spdue to the doubts about the possibility of being a hybrid betweenSpondias tub...
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| Formato: | Dissertação |
| Idioma: | pt_BR |
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Universidade Federal do Rio Grande do Norte
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| Endereço do item: | https://repositorio.ufrn.br/handle/123456789/30804 |
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| Resumo: | Spondias bahiensisP. Carvalho, Van den Berg & M. Machado is one of the five species ofSpondiasgenus present in Brazil, mainly occurring in the Northeast of Brazil. The plant was identified for many years asSpondias spdue to the doubts about the possibility of being a hybrid betweenSpondias tuberosaandSpondias mombin.S. bahiensiswas identified based on molecular biology studies involving barcode markers that confirmed the plant as a new species. The objective of this study is to address the intraspecific variability ofS. bahiensisspecies in relation to the morphological characteristics of the fruit and the fruit flavor and to resolve doubts related to the classification of the specie that could remain exist. We intend to investigate if the genetic variability in the species can be accessed with the available barcode markers. Methodology used consisted in collecting plant material (young leaves) from five species of Spondias, includingS. bahiensis, at different locations in the states of Rio Grande do Norte and Paraíba, and carrying out PCR amplifications with universal barcodes markers rbcL, matK, trnG-trnS, trnH-psbA, rpoB, and rpoC. The intraspecific variabilaidae of S. bahiensis was studied from experimental plantings of EMPARN -Agricultural Research Company of Rio Grande do Norte in the municipality of Ipanguaçu, Rio Grande do Norte. The results obtained allowed the amplification and sequencing of the barcode regions by the different markers used and the realization of phylogenetic trees that confirm, in this analysis, the taxonomic position of S. bahiensis in comparison with the other species of the genus |
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