Genotipagem e proteômica de isolados ambientais de Candida tropicalis obtidos do ambiente costeiro
Candida tropicalis is considered the second most virulent species of the genus Candida. Among the virulence factors related to this species, there is a strong production of biofilm, adhesion to human oral epithelial cells, secretion of lytic enzymes, morphogenesis and phenotipic switching. In add...
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| Formato: | doctoralThesis |
| Idioma: | pt_BR |
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Universidade Federal do Rio Grande do Norte
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| Endereço do item: | https://repositorio.ufrn.br/handle/123456789/30411 |
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| Resumo: | Candida tropicalis is considered the second most virulent species of the genus
Candida. Among the virulence factors related to this species, there is a strong
production of biofilm, adhesion to human oral epithelial cells, secretion of lytic
enzymes, morphogenesis and phenotipic switching. In addition, C. tropicalis is
osmotolerant and this characteristic is important for its persistence in the coastal
environment, causing health risk to the community. The objective of this study was to
carry out a bibliographic review on C. tropicalis, focusing on all the subjects mentioned
previously. In addition, microsatellite and MALDI-TOF/MS techniques were used to
evaluate the genotypic and phenotypic variability of 62 isolates of C. tropicalis obtained
from different geographical sites of an urban beach located in the northeast of Brazil,
during two different climatic seasons (dry and rainy seasons), besides evaluating the
population dynamics of this species in the coastal environment throughout the year.
There was a trend for isolates collected in the same period of the year to be placed in
the same cluster, by both techniques, however only 27 strains (43.5%) were placed in
the same cluster in both the microsatellite and MALDI-TOF/MS, which suggests a
relatively low correspondence between these two typing techniques. Furthermore, it
was observed that isolates obtained from the same collection timepoint were grouped
within the same cluster or in close clusters, and that isolates obtained from the same
geographic site are, in most cases, considered identical or highly related to at least
one other isolate, by both techniques, suggesting genetic relatdness. The methods
employed also demonstrated the heterogeneity of C. tropicalis in the coastal
environment. Highly related strains were found in different geographic collection sites,
demonstrating that C. tropicalis may be dispersed over long distances. In view of the
incipient use of MALDI-TOF/MS, further studies are necessary to consolidate this
technique as a yeast typing tool, when compared to the microsatellite technique, which
is consolidated as a genotyping method for Candida spp. |
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