Identification of non-zein proteins in BR473 maize protein bodies by LC-nanoESI-MS/MS

The nutritional value of maize seed is limited due to its high content of storage proteins (zeins), which are deficient in essential amino acids such as lysine and tryptophan. In a previous paper, we showed that protein bodies obtained from BR473 maize variety, developed by Embrapa (Brazilian Agricu...

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Principais autores: Bicudo, Tatiana de Campos, Bicudo, Rogério Campos, Forato, Lucimara A., Titato, Guilherme M., Colnago, Luiz A., Lanças, Fernando M.
Formato: article
Idioma:English
Publicado em: Wiley
Assuntos:
BR473 maize protein bodies
LC-nanoESI-MS/MS
Non-zein proteins
Protein identification
Endereço do item:https://repositorio.ufrn.br/handle/123456789/30364
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spelling ri-123456789-303642022-05-25T18:41:48Z Identification of non-zein proteins in BR473 maize protein bodies by LC-nanoESI-MS/MS Bicudo, Tatiana de Campos Bicudo, Rogério Campos Forato, Lucimara A. Titato, Guilherme M. Colnago, Luiz A. Lanças, Fernando M. BR473 maize protein bodies LC-nanoESI-MS/MS Non-zein proteins Protein identification The nutritional value of maize seed is limited due to its high content of storage proteins (zeins), which are deficient in essential amino acids such as lysine and tryptophan. In a previous paper, we showed that protein bodies obtained from BR473 maize variety, developed by Embrapa (Brazilian Agricultural Research Corporation), were mainly constituted by Z27 and a smaller quantity of Z50g-zeins. Besides zein proteins, other not identified protein band in the SDS/PAGE was also observed, which could indicate the presence of non-zein proteins additionally tog-zeins. In the present paper, we have demonstrated the presence of non-zein proteins in BR473 maize protein bodies by LCnanoESI-MS/MS and database searching. This fact could be related to the excellent energetic value and higher protein quality of BR473 maize grains, since high lysine concentration in some maize varieties has been related to the presence of cytoskeleton proteins that are non-zeins. We have identified the following proteins: Brittle-1 protein (chloroplast precursor), Legumin-1, glyceroldehyde-3-phosphate dehydrogenase, and elongation factor 1-a 2020-10-09T19:36:35Z 2020-10-09T19:36:35Z 2009-12-22 article BICUDO, Rogério Campos; BICUDO, Tatiana Campos; FORATO, Lucimara A.; TITATO, Guilherme M.; COLNAGO, Luiz A.; LANÇAS, Fernando M.. Identification of non-zein proteins in BR473 maize protein bodies by LC-nanoESI-MS/MS. Journal Of Separation Science, [S.L.], v. 32, n. 21, p. 3579-3584, 7 out. 2009. Disponível em: https://onlinelibrary.wiley.com/doi/full/10.1002/jssc.200900339. Acesso em: 08 set. 2020. http://dx.doi.org/10.1002/jssc.200900339 1615-9314 https://repositorio.ufrn.br/handle/123456789/30364 10.1002/jssc.200900339 en Attribution 3.0 Brazil http://creativecommons.org/licenses/by/3.0/br/ Wiley
institution Repositório Institucional
collection RI - UFRN
language English
topic BR473 maize protein bodies
LC-nanoESI-MS/MS
Non-zein proteins
Protein identification
spellingShingle BR473 maize protein bodies
LC-nanoESI-MS/MS
Non-zein proteins
Protein identification
Bicudo, Tatiana de Campos
Bicudo, Rogério Campos
Forato, Lucimara A.
Titato, Guilherme M.
Colnago, Luiz A.
Lanças, Fernando M.
Identification of non-zein proteins in BR473 maize protein bodies by LC-nanoESI-MS/MS
description The nutritional value of maize seed is limited due to its high content of storage proteins (zeins), which are deficient in essential amino acids such as lysine and tryptophan. In a previous paper, we showed that protein bodies obtained from BR473 maize variety, developed by Embrapa (Brazilian Agricultural Research Corporation), were mainly constituted by Z27 and a smaller quantity of Z50g-zeins. Besides zein proteins, other not identified protein band in the SDS/PAGE was also observed, which could indicate the presence of non-zein proteins additionally tog-zeins. In the present paper, we have demonstrated the presence of non-zein proteins in BR473 maize protein bodies by LCnanoESI-MS/MS and database searching. This fact could be related to the excellent energetic value and higher protein quality of BR473 maize grains, since high lysine concentration in some maize varieties has been related to the presence of cytoskeleton proteins that are non-zeins. We have identified the following proteins: Brittle-1 protein (chloroplast precursor), Legumin-1, glyceroldehyde-3-phosphate dehydrogenase, and elongation factor 1-a
format article
author Bicudo, Tatiana de Campos
Bicudo, Rogério Campos
Forato, Lucimara A.
Titato, Guilherme M.
Colnago, Luiz A.
Lanças, Fernando M.
author_facet Bicudo, Tatiana de Campos
Bicudo, Rogério Campos
Forato, Lucimara A.
Titato, Guilherme M.
Colnago, Luiz A.
Lanças, Fernando M.
author_sort Bicudo, Tatiana de Campos
title Identification of non-zein proteins in BR473 maize protein bodies by LC-nanoESI-MS/MS
title_short Identification of non-zein proteins in BR473 maize protein bodies by LC-nanoESI-MS/MS
title_full Identification of non-zein proteins in BR473 maize protein bodies by LC-nanoESI-MS/MS
title_fullStr Identification of non-zein proteins in BR473 maize protein bodies by LC-nanoESI-MS/MS
title_full_unstemmed Identification of non-zein proteins in BR473 maize protein bodies by LC-nanoESI-MS/MS
title_sort identification of non-zein proteins in br473 maize protein bodies by lc-nanoesi-ms/ms
publisher Wiley
publishDate 2020
url https://repositorio.ufrn.br/handle/123456789/30364
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