Avaliação do potencial toxicológico e teratogênico do extrato aquoso das cascas do cajueiro (Anacardium occidenale) utilizando-se peixe zebra (Danio rerio) como modelo experimental
The cashew tree (Anacardium occidentale) is a plant native to the Brazilian Northeast region commonly used in folk medicine due to its anti-inflammatory, astringent and healing properties. However, it is also used for abortion, but there are a lack of scientific evidence regarding its effects dur...
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| Formato: | Dissertação |
| Idioma: | pt_BR |
| Publicado em: |
Universidade Federal do Rio Grande do Norte
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| Endereço do item: | https://repositorio.ufrn.br/handle/123456789/30343 |
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| Resumo: | The cashew tree (Anacardium occidentale) is a plant native to the Brazilian Northeast
region commonly used in folk medicine due to its anti-inflammatory, astringent and
healing properties. However, it is also used for abortion, but there are a lack of scientific
evidence regarding its effects during pregnancy. Moreover, there is a lack of information
regarding chemical composition in the aqueous extracts. Considering that the use of plant
extracts during pregnancy can cause both maternal and fetal toxicity, the aim of this study
was to characterize phytochemically A. occidentale bark aqueous extract, as well its
possible toxic and teratogenic effects, using zebrafish (Danio rerio) as an experimental
model. Acute toxicity tests on embryos and females of D. rerio followed the
recommendations of the Organization for Economic Cooperation and Development. The
plant extract was prepared and phytochemical characterization was performed by Liquid
Chromatography Coupled to Mass Spectrometry, followed by tests to determine the
median lethal dose in embryos and females, behavior tests, evaluation of developmental
deformations, testing for abnormalities in DNA, estimation of the fertility rate in the
parents, and microscopic analysis of embryos and larvae exposed to Eaq. The results of
the phytochemical characterization indicate the presence of phenolic as acids derivatives
and tannis. Toxicity tests suggest a toxic effect on adult females with changes in their
behavioral profile and a tendency to decrease changes at the DNA level compared to the
control group, with no effect of the extract on fertility. In embryos exposed at 6, 12, 24-
and 48-hours post fertilization to sublethal doses of aqueous extract, there were little
teratogenic effects. However, it was observed deformation in the chorionic membrane
and delay in hatching, indicating toxic effects of the extract. The specialized metabolites
found in this species are antioxidant, but the extracts are complex mixture of chemical
compounds. In view of this, it could be concluded that A. occidentale shells aqueous
extract, to showing signs of teratogenicity to zebra fish in sublethal doses and, evidence
of toxicity in higher doses, both for embryos and adult females. |
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