Efeitos anti-inflamatório e antioxidante do extrato da folha de Spondias mombin em modelo experimental de mucosite oral
Oral mucositis (MO) is characterized as an inflammatory condition for clinically presenting erythematous and ulcerated areas. It is the most common oral complication in patients undergoing chemotherapy and / or head and neck radiation therapy, and its treatment aims to relieve painful symptoms an...
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| Formato: | doctoralThesis |
| Idioma: | pt_BR |
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Universidade Federal do Rio Grande do Norte
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| Endereço do item: | https://repositorio.ufrn.br/jspui/handle/123456789/30182 |
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| Resumo: | Oral mucositis (MO) is characterized as an inflammatory condition for clinically
presenting erythematous and ulcerated areas. It is the most common oral complication in
patients undergoing chemotherapy and / or head and neck radiation therapy, and its treatment
aims to relieve painful symptoms and induce the injury repair process. Spondias mombin is
a plant widely used in northeastern Brazil and its leaves are used in folk medicine to for the
treatment of oral and pharyngeal infections. Aim: To evaluate the antioxidant and antiinflammatory potentials of the hydroethanolic extract of the leaf of Spondias mombin
(HESM) in an experimental model of oral mucositis. Methods: A pre-clinical, in vivo, blind,
randomized and controlled trial was performed. The sample consisted of 42 male hamsters,
divided into 6 groups: Saline (S), Mechanical trauma (TM), 5 fluorouracil / Mechanical
trauma (5-FU / TM) and experimental HESM doses of 50, 100 and 200 mg / kg, administered
daily, for ten days. The animals were submitted to biochemical analyzes and the samples of
pouches of the cheek mucosa were subjected to macroscopic, histopathological and
immunohistochemical analyzes, with Cox-2, iNOS, NF-kB p50 NLS and MMP-2 markers.
The levels of IL-1β and TNF-α were analyzed by ELISA immunoreaction and the levels of
superoxide dismutase (SOD), glutathione (GSH) and malondialdehyde (MDA) were
submitted to spectroscopic analysis. Results: The HESM-treated group at a dose of 200 mg
/ kg showed the best healing effect, showing no evidence of ulceration in macroscopic
analysis (p <0.05). Histopathological analysis of this group showed reepithelialization, a mild
mononuclear inflammatory infiltrate and absence of a bleeding and edema score of 1 (1-1)
(p <0.05), in addition to a large amount of collagen fibers. Reduced immunoexpression of
Cox-2, iNOS, NF-kB p50 NLS and MMP-2; decreased levels of SOD (p <0.05), MDA (p
<0.001), IL-1β (p <0.05) and TNF-α (p <0.001); and an increase in GSH levels (p <0.01)
were also observed in this same group. Conclusion: HESM (200 mg / kg) reduced oxidative
stress and inflammation in oral mucositis induced by 5-fluorouracil in hamsters. |
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