Avaliação do potencial antioxidante, antibacteriano e antibiofilme de polissacarídeos sulfatados obtidos da alga verde Codium isthmocladum
The green macroalgae Codium isthmocladum it is a bioactive sulfated polysaccharide (SP) rich source. In addition to being found in abundance on Rio Grande do Norte coast, the SP of this alga have already been described in terms of their anticoagulant and antinociceptive potential in previous stud...
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| Formato: | doctoralThesis |
| Idioma: | pt_BR |
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Universidade Federal do Rio Grande do Norte
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| Endereço do item: | https://repositorio.ufrn.br/jspui/handle/123456789/30149 |
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| Resumo: | The green macroalgae Codium isthmocladum it is a bioactive sulfated
polysaccharide (SP) rich source. In addition to being found in abundance on Rio
Grande do Norte coast, the SP of this alga have already been described in terms
of their anticoagulant and antinociceptive potential in previous studies. In this
context, the seaweed SP were evaluated for antioxidant capacity in several in
vitro systems, as well as the potential of these compounds as possible
antibacterial and antibiofilm agents against susceptible and multi-resistant
bacteria in vitro. After collected, the seaweed was treated in order to obtain an
isolate polysaccharides-rich (crude extract). From a subsequent fractionation of
this extract, five ketone fractions were obtained (Ci 0.3, Ci 0.5, Ci 0.7, Ci 0.9 and
Ci 1.2). All fractions have a low proteins and phenolic compounds content (< 1%).
Ci 0.7 is the fraction with the highest yield and sulfate content. All polysaccharides
showed antioxidant capacity, acting as potent hydroxyl and superoxide radicals
scavengers, as well as a chelator of copper and iron ions. However, only Ci 0.7
and Ci 0.9 were able to inhibit the growth of resistant Staphylococcus aureus
(MRSA) and Escherichia coli in adjuvance to several antibiotics, especially with
gentamicin and ampicillin. Both fractions were subjected to ion exchange
chromatography and tend to generate two chromatographic peaks at different
molarities, eluted with 1.0 M and 2.0 M NaCl, respectively for Ci 0.7, and 2.0 M
and 3.0 M NaCl for Ci 0.9. Based on the data obtained from chemical and
structural analyzes, it is possible to state they are sulfated galactans. Galactans
from Ci-0.7-1M and Ci-0.9-3M showed antibacterial and antibiofilm capacity,
especially in adjuvance to antibiotics, as well as decreasing the expression
pattern of various proteins. Therefore, sulfated galactans from C. isthmocladum
are promising biomolecules in possible pharmacological treatments, especially
as an antibacterial and antibiofilm compounds. |
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