Avaliação da atividade antimicrobiana de extrato aquoso e frações derivadas de sementes de Cumaru [Amburana cearensis (Allema) A. C. Smith] associadas a antimicrobianos
The Fabaceae family is well known for its broad economic importance and various purposes. Amburana cearensis, Fabaceae, popularly known as imburana or cumaru, is widely used in popular medicine, what sustain search for novel pharmacological agents from its components. In this context, this study...
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| Formato: | doctoralThesis |
| Idioma: | pt_BR |
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Universidade Federal do Rio Grande do Norte
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| Endereço do item: | https://repositorio.ufrn.br/jspui/handle/123456789/30147 |
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| Resumo: | The Fabaceae family is well known for its broad economic importance and various
purposes. Amburana cearensis, Fabaceae, popularly known as imburana or cumaru, is widely
used in popular medicine, what sustain search for novel pharmacological agents from its
components. In this context, this study aimed to evaluate the antimicrobial and modulating
activities, as well as to characterize the crude extract and fractions obtained from A. cearensis
seeds by liquid chromatography and mass spectrometry. The aqueous extract obtained in 50
mM sodium phosphate buffer pH 8.0 was sequentially fractionated in ammonium sulfate and
subjected to characterization by Acquity UPLC system (Waters), coupled to quadrupole time
of flight mass spectrometry (QtoF, Waters). Also, antimicrobial activity was verified by
microdilution assay and the possible interactions between plant extract and antibiotics
penicillin, norfloxacin, gentamicin and antifungal fluconazole, were determined using a
subinhibitory concentration. Samples at 128 µg/mL showed inhibitory activity (p<0,05) with
possible synergistic effect when associated with antibiotics and antifungal agent against
Escherichia coli, Sthaphylococcus aureus and the yeast Candida albicans. It was possible to
identify in the crude extract and fractions several phenolic compounds, organic acids, and
some polyphenols in positive ionization mode. These results suggest that aqueous extract and
fractions of A. cearensis may present compounds with the ability to interact and act
synergistically with antimicrobial drugs, highlighting its potential as an alternative source for
the development of new antimicrobial agents. |
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