Avaliação da segurança e potencial bioativo de nanopartículas de carotenoides oriundo de melão Cantaloupe (Cucumis melo L.) administradas em modelo experimental de inflamação crônica
Carotenoids have powerful anti-inflammatory action on the human body. However, are unstable in the presence of light, acids, oxygen, and heat, which can lead to the loss of antioxidant effects related to the consumption of these molecules. Nanoencapsulation appears as a strategy to preserve or en...
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| Formato: | Dissertação |
| Idioma: | pt_BR |
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Universidade Federal do Rio Grande do Norte
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| Endereço do item: | https://repositorio.ufrn.br/jspui/handle/123456789/30033 |
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| Resumo: | Carotenoids have powerful anti-inflammatory action on the human body. However,
are unstable in the presence of light, acids, oxygen, and heat, which can lead to the
loss of antioxidant effects related to the consumption of these molecules.
Nanoencapsulation appears as a strategy to preserve or enhance the antiinflammatory action, in addition to increasing the solubility and bioavailability of
carotenoids. Therefore, it is necessary to evaluate the safety of nanoparticles for
future clinical applications. Thus, the objective of this study was to evaluate the
safety and bioactivity of nanoparticles based on crude Cantaloupe melon extract rich
in carotenoids and porcine gelatin in Wistar rats. Encapsulation was performed by
oil/water emulsification followed by freeze-drying, and porcine gelatin was used as an
encapsulating agent, and Tween 20 as a surfactant. Nanoformulation (EPG) was
characterized by different physical and chemical analyzes and evaluated for
efficiency of incorporation (EI). Toxicity was investigated through signs of general
toxicity, body weight and food consumption in male Wistar rats fed with a high
glycemic index and glycemic load (HGLI) diet treated with EPG administered by
gavage (50 mg/kg, n = 5), and crude extract rich in carotenoid (CE) (12.5 mg/kg, n =
5) for ten days, using as controls a group without treatment (n = 5) and another
treated with a standard diet (n = 5). Blood toxicity (blood count, fasting blood glucose,
triglycerides, total cholesterol, liver, and kidney function), morphology and
histopathology of the liver, small intestine, kidneys, stomach, and spleen, were
evaluated. The characterization of EPG pointed to the presence of spherical particles
with a smooth surface, an average diameter of 74 (12.7) nm, a polydispersion index
of 0.53 (0.12), and EI of 98% (1.78). For signs of general toxicity, there was no
significant difference (p> 0.05) between the initial and final weight of the rats in each
evaluated group. There was a significant increase (p <0.05) of 23% in food
consumption only in the group without treatment. The biochemical parameters
analyzed evidenced low toxicity of EPG. The results obtained for the determination of
the relative weight of the livers, spleen, small intestine, kidneys, and stomach
showed that there was no significant difference (p> 0.05) between the studied
groups. The morphological and histopathological analyzes of the liver showed acute
hepatitis in the animals of all the evaluated groups, and those treated with CE and
EPG showed improved tissue aspects. For small intestine, chronic enteritis was
observed in all animals, with an improvement in the aspect of the animals' villi and
intestinal glands treated with EPG. The stomach, spleen, and kidneys do not present
a histopathological alteration. Spearman (r) correlations and p-value of the
histopathological parameters analyzed semiquantitatively corroborated with the
aspects of the histopathological slides. The results suggest the safe application of
EPG and a bioactive effect, possibly related to the anti-inflammatory potential,
showing the safety regarding the use in future clinical studies, aiming to investigate
the efficacy in the control of inflammatory processes. |
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