Investigation of the BOLD and CBV fMRI responses to somatosensory stimulation in awake marmosets (Callithrix jacchus)

Understanding the spatiotemporal features of the hemodynamic response function (HRF) to brain stimulation is essential for the correct application of neuroimaging methods to study brain function. Here, we investigated the spatiotemporal evolution of the blood oxygen level‐dependent (BOLD) and cerebr...

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Principais autores: Hirano, Yoshiyuki, Yen, Cecil C., Liu, Junjie V., Mackel, Julie B., Merkle, Hellmut, Nascimento, George Carlos do, Stefanovic, Bojana, Silva, Afonso C.
Formato: article
Idioma:English
Publicado em: Wiley
Assuntos:
Bold
Cerebral blood volume
Functional neuroimaging
Neurovascular coupling
Non‐human primate
Somatosensory cortex
Endereço do item:https://repositorio.ufrn.br/jspui/handle/123456789/29629
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spelling ri-123456789-296292022-05-20T20:34:43Z Investigation of the BOLD and CBV fMRI responses to somatosensory stimulation in awake marmosets (Callithrix jacchus) Hirano, Yoshiyuki Yen, Cecil C. Liu, Junjie V. Mackel, Julie B. Merkle, Hellmut Nascimento, George Carlos do Stefanovic, Bojana Silva, Afonso C. Bold Cerebral blood volume Functional neuroimaging Neurovascular coupling Non‐human primate Somatosensory cortex Understanding the spatiotemporal features of the hemodynamic response function (HRF) to brain stimulation is essential for the correct application of neuroimaging methods to study brain function. Here, we investigated the spatiotemporal evolution of the blood oxygen level‐dependent (BOLD) and cerebral blood volume (CBV) HRF in conscious, awake marmosets (Callithrix jacchus ), a New World non‐human primate with a lissencephalic brain and with growing use in biomedical research. The marmosets were acclimatized to head fixation and placed in a 7‐T magnetic resonance imaging (MRI) scanner. Somatosensory stimulation (333‐μs pulses; amplitude, 2 mA; 64 Hz) was delivered bilaterally via pairs of contact electrodes. A block design paradigm was used in which the stimulus duration increased in pseudo‐random order from a single pulse up to 256 electrical pulses (4 s). For CBV measurements, 30 mg/kg of ultrasmall superparamagnetic ironoxide particles (USPIO) injected intravenously, were used. Robust BOLD and CBV HRFs were obtained in the primary somatosensory cortex (S1), secondary somatosensory cortex (S2) and caudate at all stimulus conditions. In particular, BOLD and CBV responses to a single 333‐μs‐long stimulus were reliably measured, and the CBV HRF presented shorter onset time and time to peak than the BOLD HRF. Both the size of the regions of activation and the peak amplitude of the HRFs grew quickly with increasing stimulus duration, and saturated for stimulus durations greater than 1 s. Onset times in S1 and S2 were faster than in caudate. Finally, the fine spatiotemporal features of the HRF in awake marmosets were similar to those obtained in humans, indicating that the continued refinement of awake non‐human primate models is essential to maximize the applicability of animal functional MRI studies to the investigation of human brain function 2020-07-15T00:01:49Z 2020-07-15T00:01:49Z 2017-12-29 article HIRANO, Y.; YEN, C.C.; LIU, J.V. ; MACKEL, J.B.; MERKLE, H.; NASCIMENTO, G.C. ; STEFANOVIC, B.; SILVA, Afonso C.. Investigation of the BOLD and CBV fMRI responses to somatosensory stimulation in awake marmosets ( ). NMR in Biomedicine, v. 31, p. e3864, 2018. Disponível em: https://onlinelibrary.wiley.com/doi/abs/10.1002/nbm.3864. Acesso em: 14 jul. 2020. https://doi.org/10.1002/nbm.3864 0952-3480 https://repositorio.ufrn.br/jspui/handle/123456789/29629 10.1002/nbm.3864 en Attribution 3.0 Brazil http://creativecommons.org/licenses/by/3.0/br/ Wiley
institution Repositório Institucional
collection RI - UFRN
language English
topic Bold
Cerebral blood volume
Functional neuroimaging
Neurovascular coupling
Non‐human primate
Somatosensory cortex
spellingShingle Bold
Cerebral blood volume
Functional neuroimaging
Neurovascular coupling
Non‐human primate
Somatosensory cortex
Hirano, Yoshiyuki
Yen, Cecil C.
Liu, Junjie V.
Mackel, Julie B.
Merkle, Hellmut
Nascimento, George Carlos do
Stefanovic, Bojana
Silva, Afonso C.
Investigation of the BOLD and CBV fMRI responses to somatosensory stimulation in awake marmosets (Callithrix jacchus)
description Understanding the spatiotemporal features of the hemodynamic response function (HRF) to brain stimulation is essential for the correct application of neuroimaging methods to study brain function. Here, we investigated the spatiotemporal evolution of the blood oxygen level‐dependent (BOLD) and cerebral blood volume (CBV) HRF in conscious, awake marmosets (Callithrix jacchus ), a New World non‐human primate with a lissencephalic brain and with growing use in biomedical research. The marmosets were acclimatized to head fixation and placed in a 7‐T magnetic resonance imaging (MRI) scanner. Somatosensory stimulation (333‐μs pulses; amplitude, 2 mA; 64 Hz) was delivered bilaterally via pairs of contact electrodes. A block design paradigm was used in which the stimulus duration increased in pseudo‐random order from a single pulse up to 256 electrical pulses (4 s). For CBV measurements, 30 mg/kg of ultrasmall superparamagnetic ironoxide particles (USPIO) injected intravenously, were used. Robust BOLD and CBV HRFs were obtained in the primary somatosensory cortex (S1), secondary somatosensory cortex (S2) and caudate at all stimulus conditions. In particular, BOLD and CBV responses to a single 333‐μs‐long stimulus were reliably measured, and the CBV HRF presented shorter onset time and time to peak than the BOLD HRF. Both the size of the regions of activation and the peak amplitude of the HRFs grew quickly with increasing stimulus duration, and saturated for stimulus durations greater than 1 s. Onset times in S1 and S2 were faster than in caudate. Finally, the fine spatiotemporal features of the HRF in awake marmosets were similar to those obtained in humans, indicating that the continued refinement of awake non‐human primate models is essential to maximize the applicability of animal functional MRI studies to the investigation of human brain function
format article
author Hirano, Yoshiyuki
Yen, Cecil C.
Liu, Junjie V.
Mackel, Julie B.
Merkle, Hellmut
Nascimento, George Carlos do
Stefanovic, Bojana
Silva, Afonso C.
author_facet Hirano, Yoshiyuki
Yen, Cecil C.
Liu, Junjie V.
Mackel, Julie B.
Merkle, Hellmut
Nascimento, George Carlos do
Stefanovic, Bojana
Silva, Afonso C.
author_sort Hirano, Yoshiyuki
title Investigation of the BOLD and CBV fMRI responses to somatosensory stimulation in awake marmosets (Callithrix jacchus)
title_short Investigation of the BOLD and CBV fMRI responses to somatosensory stimulation in awake marmosets (Callithrix jacchus)
title_full Investigation of the BOLD and CBV fMRI responses to somatosensory stimulation in awake marmosets (Callithrix jacchus)
title_fullStr Investigation of the BOLD and CBV fMRI responses to somatosensory stimulation in awake marmosets (Callithrix jacchus)
title_full_unstemmed Investigation of the BOLD and CBV fMRI responses to somatosensory stimulation in awake marmosets (Callithrix jacchus)
title_sort investigation of the bold and cbv fmri responses to somatosensory stimulation in awake marmosets (callithrix jacchus)
publisher Wiley
publishDate 2020
url https://repositorio.ufrn.br/jspui/handle/123456789/29629
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