Commiphora leptophloeos (Mart.) J.B. Gillett (Burseraceae): estudo fitoquímico, toxicidade e avaliação do potencial antiinflamatório e antimicrobiano
The specie Commiphora leptophloeos (Burseraceae) is a native plant from Brazil, belongs to caatinga biome and is known popularly as “imburana-de-espinho” and “imburana-decambão”. The ethnopharmacology studies mention the use of this specie in the treatment of inflammations and infections. In this...
Na minha lista:
| Autor principal: | |
|---|---|
| Outros Autores: | |
| Formato: | Dissertação |
| Idioma: | pt_BR |
| Publicado em: |
Universidade Federal do Rio Grande do Norte
|
| Assuntos: | |
| Endereço do item: | https://repositorio.ufrn.br/jspui/handle/123456789/29460 |
| Tags: |
Adicionar Tag
Sem tags, seja o primeiro a adicionar uma tag!
|
| Resumo: | The specie Commiphora leptophloeos (Burseraceae) is a native plant from Brazil, belongs to
caatinga biome and is known popularly as “imburana-de-espinho” and “imburana-decambão”. The ethnopharmacology studies mention the use of this specie in the treatment of
inflammations and infections. In this context, the present study aimed to evaluate the toxicity,
anti-inflammatory and antimicrobial activity in non-clinical in vitro and in vivo assays of leaf
and stem bark extracts, and to isolate, identify and quantify chemical markers. In the
phytochemical study, the hydroethanolic extract was prepared by maceration and the
fractionation was performaded by liquid-liquid partition (dichloromethane, ethyl acetateAcOEt and n-buthanol-BuOH fractions). Isolation, structural elucidation and characterization
of the extracts were performed by CPC, LC-MS, FIA-ESI-IT-MS/MS and 1H NMR and
quantification of the content of markers in the extracts was made by HPLC-DAD and HPLCELSD. The toxicity of both extracts was evaluated by MTT and flow cytometry assays and in
vivo by the acute toxicity test. The in vitro anti-inflammatory activity was evaluated using
LPS-induced nitric oxide assay and in viv by carrageenan-induced paw edema and oral
zymosan induced air pocket models. The antimicrobial activity was measured by the
determination of minimum inhibitory concentration (MIC) and virtual screening of the
isolated compounds was performed by in silico studies. From leaves extract, 2 flavonoids
were isolated by CPC and 16 compounds were characterized by Mass spectrometry such as
phenolic acids, glycosylated flavonoids derivatives of quercetin, luteolin and apigenin, and
condensed tannins derivatives of catechin. From the stem bark extract, 2 tannins were isolated
by CPC and 8 compounds were characterized as phenolic acids and procyanidins. The leaves
extract at 200 µg/mL and stem bark extract at concentrations of 1, 10, 100 and 200 µg/mL
demonstrated in vitro anti-inflammatory effect in the LPS-induced nitric oxide assay. In the
carrageenan-induced paw edema of model, the extracts of leaves and stem bark at the doses of
100, 200 and 400 mg/kg, by oral route, significantly reduced the edema followed by reduction
of myeloperoxidase (MPO) and in the zymosan-induced model of pouch-air, at doses tested,
significantly reducing (p < 0.001), cell migration, total protein concentration,
myeloperoxidase (MPO), and malondialdehyde (MDA) and the proinflammatory cytokine
TNF-α and increased the production of the anti-inflammatory cytokine IL-10. In the
evaluation of the antimicrobial activity, the extracts of the barks and the AcOET and BuOH
fractions at concentrations of 20 to 100 μg/mL demonstrated a fungistatic and bactericidal
effect. The in silico study indicated possible ligands of type B dimeric procyanidin and
isovitexin related to anti-inflammatory and antimicrobial activity. The results obtained are
unprecedent for the specie, justify its use in folk medicine and reveals that extracts of leaves
and stem bark have a therapeutic potential for the development of herbal products with antiinflammatory and antimicrobial properties. |
|---|