Desenvolvimento e caracterização de formulações larvicidas contendo derivado do ácido cinâmico para o combate ao Aedes aegypti
The main strategy for controlling diseases transmitted by the Aedes aegypti mosquito is the elimination of the vector, mainly in its larval stage. Commercial larvicides and insecticides shows environmental toxicity. In this context, natural products show as alternatives to solve this problem. The...
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| Formato: | Dissertação |
| Idioma: | pt_BR |
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Universidade Federal do Rio Grande do Norte
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| Endereço do item: | https://repositorio.ufrn.br/jspui/handle/123456789/29458 |
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| Resumo: | The main strategy for controlling diseases transmitted by the Aedes aegypti
mosquito is the elimination of the vector, mainly in its larval stage. Commercial
larvicides and insecticides shows environmental toxicity. In this context, natural
products show as alternatives to solve this problem. The aim of this study was to
develop and characterize formulations containing pentyl cinnamate and evaluate its
larvicidal activity for Aedes aegypti. The pentyl cinnamate was obtained by
semisynthesis through the esterification of cinnamic acid. This compound was
characterized and its purity determined by NMR 1H e 13C, GC-MS and UHPLCMS/MS. Pentyl cinnamate activity tests were performed on L3 larvae and on adult
mosquitoes of A. aegypti, obtaining a larvicidal activity with LC50 of 19.9 μg.mL-1
, and
against adult mosquitoes at a dose of 200 μg per bottle test, 80.8 ± 3.8% mortality in 2
h. Nanoemulsions were produced with PHOSAL® 50+ (PHO), LIPOID® S 20 (L20) and
LIPOID® S 75-3 (L75) lecithins by homogenization with mechanical agitation and ultra
turrax. The nanoemulsions were subjected to spray drying to obtain solid microparticles,
which were characterized by FTIR, SEM and thermal analysis by DSC and TG. The
nanoemulsions obtained by agitation with turrax showed smaller droplet sizes, being
that of PHO: 81.0 ± 1.8 nm; L20: 120.4 ± 4.9 nm and L75 with 150.9 nm. The PHO
nanoemulsions proved to be the most stable during 30 days of observation, they were
also the ones that showed higher content of pentyl cinnamate whith 66.0 ± 0.5%. The
images obtained by SEM showed that the microparticles formed by the different
lecithins had a similar aspect between them. In the FTIR spectra, stretching signals
characteristic of the pentyl cinnamate and excipients were observed and by DSC and
TG techniques it was possible to see an increase in the stability of the compound
incorporated in solid microparticles. The preliminary study of toxicity in zebrafish
showed evidence of toxicity of pentyl cinnamate in low concentrations, however, future
studies must be carried out for the influence of the solvent in this type of model. The
nanoemulsions and microparticles produced proved to be suitable for making the use of
this ester in water with potential use as a larvicide and adulticide feasible, opening
perspectives for studies with other compounds. |
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