Efeito da fotobiomodulação na proliferação e viabilidade de células endoteliais da veia umbilical humana (HUVEC)
The aim of this study was to evaluate, through in vitro experiments, the effect of photobiomodulation using low-level laser irradiation on the proliferation and viability of human endothelial cells. Human umbilical vein endothelial cells (HUVEC) were cultured and divided into four groups: C - con...
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| Formato: | Dissertação |
| Idioma: | pt_BR |
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| Endereço do item: | https://repositorio.ufrn.br/jspui/handle/123456789/28174 |
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| Resumo: | The aim of this study was to evaluate, through in vitro experiments, the effect of
photobiomodulation using low-level laser irradiation on the proliferation and viability
of human endothelial cells. Human umbilical vein endothelial cells (HUVEC) were
cultured and divided into four groups: C - control (without irradiation) and L1, L4 and
L7.5 groups - cells irradiated with doses of 1, 4, and 7.5 J/cm², respectively, of diode
laser (InGaAlP, wavelength of 660 nm, power of 30 Mw, in a single dose). The
metabolic activity of the cells was assessed at the 24, 48 and 72 h after irradiation by the
Alamar Blue assay. Cell viability and proliferation were also evaluated at 72 h through
the Live/Dead, Annexin V/PI assays, cell cycle phase analysis and Ki67 protein
immunostaining. Data from the Alamar Blue assay showed that at 72 h all irradiated
groups exhibited significantly greater reduction percentages than the non-irradiated
control group (p <0.01), the difference being even more evident in L4 and L7.5 (p
<0.001). The analysis of cell viability by Live/Dead demonstrated a large number of
viable cells in all groups, which was also observed through Annexin V/PI
immunostaining, which showed a high percentage of cell viability in the studied groups
(C: 95.2%, L1: 96.1%, L4: 96.1%, L7.5: 96.9%). The evaluation of the phases of the
cell cycle showed that the irradiated groups exhibited a gradual increase of the
percentage of cells in the proliferative phases of the cycle (S and G2/M), in a dosedependent manner (C: 36.9%, L1: 38.0 %, L4: 38.3%, L7.5: 40.4%). Irradiation
promoted a significant increase in Ki67 nuclear protein expression, with a more
pronounced result when the G7.5 group was compared with the other groups. Taken
together, the results of this study demonstrated that photobiomodulation, especially at a
dose of 7.5 J/cm², promoted increased proliferation and viability of HUVEC cells,
which may suggest the potential use of the laser in the parameters studied as a stimulus
to endothelialization in the vascular tissue engineering techniques. |
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