Avaliação do efeito in vitro, in vivo e ex vivo do picolinato de cromo
Chromium picolinate (PICCr) is a complex made up of three picolinic acid residues (in salt form) linked by coordination to a chromium III atom. PICCr supplementation by athletes and others has been increasing every year and without a determined dose. The hypothesis of this study is the safety of...
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| Formato: | Dissertação |
| Idioma: | pt_BR |
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Brasil
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| Endereço do item: | https://repositorio.ufrn.br/jspui/handle/123456789/28017 |
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| Resumo: | Chromium picolinate (PICCr) is a complex made up of three picolinic acid
residues (in salt form) linked by coordination to a chromium III atom. PICCr
supplementation by athletes and others has been increasing every year and without
a determined dose. The hypothesis of this study is the safety of prescribing varying
doses of chromium picolinate. After commercial acquisition of PICCr capsules from
two different countries, England and Brazil, this work began by checking by Atomic
Absorption Spectrometry, the masses contained in these capsules. We found a
higher concentration of the mineral in the capsules of English origin, which was
selected as a sample for the research. Four cell lines: murine macrophages (RAW264.7), Chinese hamster ovary (CHO) cells, murine fibroblast (3T3) and human
hepatic adecarcinoma (HepG2) cells were exposed to varying concentrations of
PICCr (5 to 20 µg /mL) CHO, 3T3 and HepG2 were found to have their ability to
reduce (10 to 80%) decreased MTT (3- (4,5-dimethylthiazol-2-yl) 2,5-diphenyl
tetrazole). RAW 264.7 cells were not affected in this analysis. HepG2 cells when
exposed to PICCr (20 to 50 µg / mL) had micronucleus numbers significantly similar
to cells that were not exposed to PICCr. RAW 264.7 cells showed a decrease in nitric
oxide release when they were exposed to bacterial wall liposaccharides (LPS). In in
vivo assays, male Swiss mice were exposed to different doses of PICCr (1000 and
2000 µg / mL) for 15 days. After this period, it was found that the treated animals had
lower weight gain and decreased plasma cholesterol, triglycerides and glucose
levels. They did not present macroscopic or histological changes in the hepatic and
renal tissues. Treated animals were also found to have 50% decrease in serum
plasma iron levels. However, metallomics analyzes show that iron and hepatic tissue
iron levels have not been affected, however, human studies are necessary to confirm
these observations, and may boost future studies to better understand these effects
and add economic and pharmacological value to the study. PICCr. |
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