Avaliação da atividade tripanossomicida de nanopartículas de prata e o polissacarídeo xilana contra formas epimastigotas de Trypanosoma cruzi
Chagas disease or American trypanosomiasis is caused by the protozoan Trypanosoma cruzi. The current treatment comprises the use of at least two drugs, benzonidazole (BNZ) and nifurtimox, which present high toxicity and inefficiency in the chronic phase of the disease, which induces the search fo...
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| Formato: | doctoralThesis |
| Idioma: | pt_BR |
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Brasil
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| Endereço do item: | https://repositorio.ufrn.br/jspui/handle/123456789/27921 |
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| Resumo: | Chagas disease or American trypanosomiasis is caused by the protozoan
Trypanosoma cruzi. The current treatment comprises the use of at least two drugs,
benzonidazole (BNZ) and nifurtimox, which present high toxicity and inefficiency in the
chronic phase of the disease, which induces the search for more effective therapeutic
alternatives. Studies have shown that the green synthesis of silver nanoparticles with
polysaccharides has potentiated the biological activity of both polysaccharides and
silver. Therefore, the xylan polysaccharide was extracted from corn cob using an
environmentally friendly method and was used for the synthesis of silver nanoparticles
(NX). NX-verified atomic force microscopy studies showing spherical shape and mean
size (~ 40 nm). Infrared and Raman spectroscopy and coupled plasma atomic
emission spectrometry confirmed the presence of silver (~ 19%) and xylan in the NX.
T. cruzi parasites lost 95% of their ability to reduce MTT (3-bromide (4,5-
dimethylthiazol-2-yl) -2,5-diphenyltetrazolium) when incubated with nano xylan (100
µg/mL). Flow cytometric analysis showed that 98% of the parasites were positive for
propidium iodide when incubated for 24 h with NX (100 µg/mL). The data show that
nano xylan induces parasite necrosis and is a strong candidate for preclinical studies
with animals infected with Trypanosoma cruzi. |
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