Avaliação da atividade biológica de células-tronco da polpa dental humana submetidas ao laser de baixa intensidade
The low-level laser therapy (LLLT) has been used in order to improve wound healing and tissue regeneration. The literature shows a positive effect of LLLT on cell proliferation, but little is known about its effectiveness on the proliferation of dental pulp stem cells. The aim of this study was to e...
Na minha lista:
| Autor principal: | |
|---|---|
| Outros Autores: | |
| Formato: | Dissertação |
| Idioma: | pt_BR |
| Publicado em: |
Universidade Federal do Rio Grande do Norte
|
| Assuntos: | |
| Endereço do item: | https://repositorio.ufrn.br/jspui/handle/123456789/27481 |
| Tags: |
Adicionar Tag
Sem tags, seja o primeiro a adicionar uma tag!
|
| Resumo: | The low-level laser therapy (LLLT) has been used in order to improve wound healing and tissue regeneration. The literature shows a positive effect of LLLT on cell proliferation, but little is known about its effectiveness on the proliferation of dental pulp stem cells. The aim of this study was to evaluate the effect of irradiation LLLT on biological activity of dental pulp stem cells from permanent teeth (DPSCs). Dental pulp extracts were isolated from healthy five third molars removed by surgical and/or orthodontic indication. After enzymatic digestion, the cells were examined and cultured in αMEM supplemented with antibiotics and 15% fetal bovine serum. On the third subculture, the cells were either irradiated with a laser diode InGaAlP, using two different energy densities (0.5 J/cm2 - 16 seconds and 1.0 J / cm ² - 33 seconds), wavelength of 660nm and power of 30mW. A new irradiation using the same parameters was performed 48 hours after the first. A control group (non-irradiated) was kept under the same experimental conditions of culture. Cell proliferation was evaluated by Trypan blue exclusion method and by measuring mitochondrial activity using the MTT-based cytotoxicity assay, at intervals of 24, 48, 72 and 96 h after the first laser application. Events of the cell cycle were evaluated in the same intervals, and the events related to cell death were analyzed by flow cytometry in the ranges of 24 to 72 hours. Data from cell counts were submitted to non-parametric Kruskal-Wallis and Mann-Whitney test, considering a confidence interval of 95%. The results showed that the both groups irradiated exhibited an upward cell proliferation curve, with statistically significant difference (p < 0.05) compared to the control group in intervals of 72 and 96 hours. No significant changes were observed in cell viability throughout the experiment. The distribution of the cells in the cell cycle phases was consistent with proliferating cells in all three groups. The results of growth curve, MTT, Anexin/PI and cell cycle were concordant, then it is possible conclude that the LLLI, especially with the dose of 1,0J/cm2, it is a therapy of great importance for the future of tissue engineering and regenerative medicine involving stem cells, once in this study it contributed to the growth and viability of DPSCs. |
|---|