Avaliação das potencialidades terapêuticas do óleo de rã-touro (Rana catesbeiana Shaw) e de diferentes sistemas nanoestruturados desenvolvidos a base de óleo de rã-touro
Bullfrog oil is a sub-product obtained from the adipose tissue of the amphibious Rana catesbeiana Shaw, which, due to literature reports around its chemical composition, it has been a target of several studies regarding its therapeutic proprieties. Thus, the aim of this study was to evaluate the...
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| Formato: | doctoralThesis |
| Idioma: | pt_BR |
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| Endereço do item: | https://repositorio.ufrn.br/jspui/handle/123456789/27360 |
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| Resumo: | Bullfrog oil is a sub-product obtained from the adipose tissue of the amphibious
Rana catesbeiana Shaw, which, due to literature reports around its chemical
composition, it has been a target of several studies regarding its therapeutic
proprieties. Thus, the aim of this study was to evaluate the therapeutic properties
and cytotoxocity of the bullfrog oil and to develop different bullfrog oil-based
nanostructured systems. Initially, a new oil extraction method was proposed,
following its physicochemical and chemical characterization. The thermo-oxidative
characterization was also performed. In addition, technological approaches were
used to develop different nanosystems (a nanoemulsion for oral use, a
nanoemulsion for topical use, a microemulsion and nanocapsules), following the
evaluation of their therapeutic activities on the inflammatory process as well as
their in vitro cytotoxicity against healthy and tumor cells. In vivo experiments were
performed to confirm the bullfrog oil’s and nanocapsules’ anti-inflammatory
activity. The physicochemical characterization showed that the extraction by
heating allowed the obtainment of product with adequate quality for further use.
The chemical characterization showed the presence of saturated and unsaturated
fatty acids. All nanostructured systems were successfully developed and, both
systems and in natura bullfrog oil did not promote toxicity against healthy cells.
Additionally, the anti-inflammatory activity was confirmed. The performed
experiments using tumor cells showed that the bullfrog oil and the nanosystems
were able to induce a reactive oxygen species (ROS) over production leading to
cell death by apoptosis (when in natura oil was tested) or necrosis (when the
systems were tested). Finally, this study allowed us to elucidate different biological
activities of the bullfrog oil, the ability of the nanostructured systems in increasing
the therapeutic effects, without interfering in the oil biocompatibility and, lastly,
allowed us to describe unprecedented data around the bullfrog oil antiinflammatory and antitumor pathways, which were involved the inhibition of the
inflammatory mediators production and ROS over production, respectively. In
conclusion, it was possible to prove that the bullfrog oil is a potent bioactive
product able to act on inflammatory processes and with potential activity on
melanoma treatment. |
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