Atividade imunoestimulatória e anticâncer de galactanas sulfatadas de Caulerpa cupressoides var. flabellata
Marine seaweeds are rich sources of compounds with pharmacological properties. Among these compounds, sulfated polysaccharides (SP) are known for their structural diversity, high content and because they present great potential of use in several human activities. For these reasons, studies with S...
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| Formato: | doctoralThesis |
| Idioma: | pt_BR |
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Brasil
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| Endereço do item: | https://repositorio.ufrn.br/jspui/handle/123456789/27359 |
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| Resumo: | Marine seaweeds are rich sources of compounds with pharmacological
properties. Among these compounds, sulfated polysaccharides (SP) are known
for their structural diversity, high content and because they present great potential
of use in several human activities. For these reasons, studies with SP have
gained prominence in biomedical research. Thus, the aim of this work was to
characterize structurally the SP from Caulerpa cupressoides var. flabellata and
to evaluate its immunostimulatory and anticancer potential, in vitro. For this, the
SP were extracted and characterized by nuclear magnetic resonance (NMR)
spectroscopy. In addition, its immunostimulatory and anticancer effects were
evaluated by quantification of different inflammatory mediators in RAW 264.7
murine macrophages, as well as their inhibitory effects on tumorigenic markers
in murine melanoma B16-F10 cells, respectively. NMR analysis revealed that the
polysaccharide fractions of C. cupressoides are rich in galactans, consisting of
units of sulfated and pyruvated β-D-galactopyranoses. These SP had no cytotoxic
effects on cell lines tested. However, they promoted significant increases in the
production of inflammatory mediators: nitric oxide, reactive oxygen species and
cytokines TNF-α and IL-6. With respect to anticancer activity, PS inhibited
migration, melanin production and clonogenic formation in B16-F10 cells.
Together, the results of this study showed that C. cupressoides sulfated
galactans may play a potent immunostimulatory and anticancer effect, with
potential use in new products with biomedical and biotechnological applications.
However, additional studies should be performed to elucidate the cellular and
molecular mechanisms involved in the observed responses, as well as to verify
these effects in other study models. |
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