Caracterização do efeito de uma fração enriquecida em saponinas obtida de Ziziphus joazeiro em isolados de Candida spp. oriundos de pacientes com candidemia com ênfase na expressão dos fatores de virulência
Bloodstream infections represent an important public health problem, with attributed mortality of 40-60%. In addition to their known in vitro virulence potential, Candida spp. have been drawing attention to the growing number of reports of resistance to antifungal drugs, representing a serious pr...
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| Formato: | Dissertação |
| Idioma: | pt_BR |
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Brasil
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| Endereço do item: | https://repositorio.ufrn.br/jspui/handle/123456789/27143 |
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| Resumo: | Bloodstream infections represent an important public health problem, with
attributed mortality of 40-60%. In addition to their known in vitro virulence potential,
Candida spp. have been drawing attention to the growing number of reports of
resistance to antifungal drugs, representing a serious problem for the treatment of
infections caused by these microorganisms. This study aimed to evaluate the
antifungal susceptibility of Candida spp. from patients with Candida bloodstream
infection against synthetic antifungal compounds, as well as an enriched fraction
obtained from the extract of Ziziphus joazeiro. We also investigated the
interference of this natural product on the expression of Candida spp. virulence
factors. Material and methods: Seventy Candida spp. isolates from blood cultures
were analyzed. The microdilution broth technique was performed to investigate the
susceptibility of the isolates against synthetic antifungals. Subsequently, 50
isolates were selected and the evaluation of the antifungal activity of the fraction
was performed. A total of 27 isolates were selected for virulence factor expression
assays in the absence and presence of the fraction. None of the isolates
presented resistance to amphotericin B and caspofungin. Four isolates of C.
albicans and 1 isolate of C. glabrata were resistant to fluconazole, whereas 3
isolates of C. albicans, 1 of C. tropicalis and 2 of C. parapsilosis complex showed
dose-dependent susceptibility to this drug. Four isolates of C. albicans and 1 of C.
tropicalis showed a dose-dependent susceptibility to itraconazole. The isolates
identified as C. albicans and C. glabrata were susceptible to the fraction with
MFCs ranging from 5000-10000 μg/mL. The strains treated with the fraction
showed a reduction of about 26% in the capacity of adhesion buccal epithelial
cells, a lower production of phospholipase, haemolisyns and a decrease in
filamentation capacity. The enriched fraction of Z. joazeiro presents significant
antifungal activity, being able to interfere with the expression of virulence factors in
vitro of this group of yeasts. Further studies should be performed in order to
evaluate the mechanisms of action involved. |
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