Atividade anticoagulante in vitro de galactanas sulfatadas obtidas da alga verde Udota flabellum (J.Ellis & Solander) M.Howe
The main known anticoagulant drug is the sulfated polysaccharide named heparin. However, the use of heparin can cause side effects such as the development of thrombocytopenia, arterial embolism, bleeding complications. Therefore, new anticoagulants that have fewer side effects than heparin are be...
Wedi'i Gadw mewn:
| Prif Awdur: | |
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| Awduron Eraill: | |
| Fformat: | Dissertação |
| Iaith: | pt_BR |
| Cyhoeddwyd: |
Brasil
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| Pynciau: | |
| Mynediad Ar-lein: | https://repositorio.ufrn.br/jspui/handle/123456789/26823 |
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| Crynodeb: | The main known anticoagulant drug is the sulfated polysaccharide named
heparin. However, the use of heparin can cause side effects such as the development
of thrombocytopenia, arterial embolism, bleeding complications. Therefore, new
anticoagulants that have fewer side effects than heparin are being sought. Many
seaweeds are known to synthesize sulfated polysaccharides with anticoagulant
activity. Therefore, in this paper, sulfated polysaccharide-rich extracts were obtained
from 22 tropical seaweeds (4 red, 11 brown, and 7 green) found in northeastern Brazil
and evaluated as anticoagulant agents. Fifteen extracts had anticoagulant activity,
including all extracts of green seaweeds. Udotea flabellum extract was the most
potent, requiring only 3 µg to double the plasma coagulation time in the activated
partial thromboplastin time (APTT) test. A similar result was obtained with 1 µg of
heparin. Two sulfated homogalactans F-I (130 kDa) and F-II (75 kDa) with high
anticoagulant activity were obtained from this extract using several bio-guided
purification steps. Their anticoagulant activities were close to that of heparin. F-I and
F-II (0.5–10 μg/mL) were not able to directly inhibit thrombin. In the presence of
antithrombin, F-I (0.5 μg/mL) was more effective than heparin (0.5 μg/mL) in inhibiting
thrombin, while F-II showed similar effects as heparin. Taken together, the results
provide strong evidence for the anticoagulant potential of sulfated homogalactans
from U. flabellum. |
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