Efeito da membrana quitosana com complexo de Zinco-vanilina no tratamento de lesões cutâneas em ratos diabéticos
The treatment of chronic wounds is still considered a public health problem, when it affects groups of risks as diabetics and becomes a great clinical challenge. In this work, we evaluated the healing effects of a new zinc complex associated with a chitosan membrane, tested in cutaneous wounds of...
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| Formato: | Dissertação |
| Idioma: | pt_BR |
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Brasil
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| Endereço do item: | https://repositorio.ufrn.br/jspui/handle/123456789/26701 |
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| Resumo: | The treatment of chronic wounds is still considered a public health problem, when it affects
groups of risks as diabetics and becomes a great clinical challenge. In this work, we evaluated
the healing effects of a new zinc complex associated with a chitosan membrane, tested in
cutaneous wounds of diabetic rats. Wistar rats were submitted to induction of diabetes with
streptozotocin 40 mg / kg (i.v.). On the seventh day after diabetic induction, a circular skin
excision (1.0 cm) with punch was performed. The lesions were treated with the pure chitosan
membrane and with the membrane associated with the Zinc-vanillin complex (CS-ZPV1 and
CS-ZPV2) at two concentrations, 7.36 x 10-7 mol cm-2 and 1.22 x 10-6 mol cm -2,
respectively. The samples were submitted to macroscopic, histopathological, cytokine (TNFα, IL-1β and IL-10) and reverse transcriptase polymerase chain reaction (RtPCR) (TGFβ and
VEGF) assays. The analysis revealed reepithelialization, angiogenic stimulation, collagen
deposition and expression of proteins that aid in the healing process in a significant proportion
in the groups treated with the CS-ZPV membrane compared to the groups without treatment.
Treatment with CS-ZPV reduced the levels of TNF-α, IL-1β and increased IL-10, also
increased the gene expression of TGFβ and VEGF. The evaluated parameters suggest that CSZPV in the two concentrations tested may be effective in the treatment of chronic wounds. |
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