Perfil de resposta imunológica inata de pacientes infectados pelo vírus Zika
The receptors of the innate immunity, mainly Toll like receptors (TLRs) and RIG like receptors (RLRs) are important molecules for the initial recognition of Zika virus and modulation of protective immune response with production of type 1 IFN. Recent Zika virus epidemics presented the main conseq...
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| Formato: | Dissertação |
| Idioma: | por |
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Brasil
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| Endereço do item: | https://repositorio.ufrn.br/jspui/handle/123456789/26354 |
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| Resumo: | The receptors of the innate immunity, mainly Toll like receptors (TLRs) and RIG like
receptors (RLRs) are important molecules for the initial recognition of Zika virus and
modulation of protective immune response with production of type 1 IFN. Recent Zika
virus epidemics presented the main consequences the increase in the number of
cases of Guillain-Barré syndrome and the emergence of congenital Zika syndrome,
which may result in microcephaly and other neurological damage. Immunological
mechanisms that confer protection or pathology during infection by this virus have
not yet been elucidated. Thus, the aim of the present study was to evaluate the
profile of the innate immune response in patients during acute Zika virus infection.
For this, the expression of messenger RNA (TLR3, TLR7, TLR8, TLR9), RLR MDA-5
(Melanoma Differentiation-Associated protein 5), TRIF adapter molecules (Toll / IL-1
Receptor (IFN-γ) and cytokines (IL-6, IL-12, IFN-α, TNF-α, IFN-γ and IFN-γ).
Peripheral blood mononuclear cells (PBMC) from 30 patients with symptoms of acute
Zika virus infection with diagnosis confirmed by real-time RT-PCR (qRT-PCR) and
nine uninfected healthy individuals were used to quantify the immune response
profile. Patients with acute Zika virus infection had high expression of TLR3, IFN-α,
IFN-β and IFN-γ when compared to healthy controls. In addition, there was a positive
correlation between TLR3 expression in relation to IFN-α and IFN-β. On the other
hand, patients infected by zika virus showed reduced expression of TLR8, Myd88
and TNF-α. Similar expression of TLR7, TLR9, MDA-5, TRIF, IL-6 and IL-12 was
observed between the group of patients infected with zika virus and control subjects.
Our results indicate that acute infection (up to 5 days after the onset of symptoms) by
the Zika virus induces the production of IFN-γ, IFN-α and IFN-β, mainly via TLR3-
dependent pathway. However, patients infected by the zika virus showed a reduction
in the expression of TLR8, Myd88 and TNF-α, molecules also involved in antiviral
immunity. |
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