Imunoexpressão das proteínas APE1, XRCC1, p53 e Ki67 em carcinoma de células escamosas de língua oral
Oral squamous cells carcinoma (OSCC) results from processes of decontrol of cellular events caused by mutations due genotoxic agents, which may lead, among other factors, to loss of control of the cellular proliferation process, being considered as one of the precursors of oral cancer. Searching...
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| Formato: | doctoralThesis |
| Idioma: | por |
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| Endereço do item: | https://repositorio.ufrn.br/jspui/handle/123456789/25470 |
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| Resumo: | Oral squamous cells carcinoma (OSCC) results from processes of decontrol of
cellular events caused by mutations due genotoxic agents, which may lead, among
other factors, to loss of control of the cellular proliferation process, being considered
as one of the precursors of oral cancer. Searching biomarkers for OSCC is the target
of several studies, among the markers it is highlighted the DNA repair proteins, such
as XRCC1 and APE1, and cell cycle proteins, such as p53 and Ki67. Thus, the aim
of this study was to analyze the immunohistochemical expression of APE1, XRCC1
and the proteins involved in the cell cycle, p53 and ki67, associating them with
clinical and histopathological prognostic parameters in oral tongue squamous cell
carcinoma (OTSCC), in order to contribute to the better understanding of the
participation of these proteins in the development of this neoplasia. The
immunohistochemical expression of APE1 and XRCC1 was evaluated
semiquantitatively and the expression of p53 and ki67 quantitatively, in 58 cases of
OTSCC. Clinical data were collected from the medical records of each patient and
the histopathological grading of Brandwein-Gensler was carried out for each case.
For the statistical analysis, Chi-square and Fisher's exact test were performed, and
significance was set at p <0.05. The majority of cases showed high
immunoexpression of APE1 (n = 36; 62.1%), as well as of XRCC1 (n = 38; 65.5%). In
relation to the Ki67 and p53 proteins, there was an equal distribution when the cases
were categorized into low and high expression (n = 29, 50%). XRCC1
immunoexpression was significantly higher in cases of early stage lesions I and II (n
= 23; 62.2%) compared to advanced stages III and IV (n = 16, 80%, p = 0.05). The
Immunoexpression of p53 was significantly higher in cases of advanced lesion (n =
19; 65.5%) and low in early stages (n = 17, 60.7%, p = 0.047). None of the studied
proteins showed association with each other, nor with the other clinical parameters
and histopathological grading. Despite the significant association of the highest
XRCC1 immunoexpression with better clinical staging and of p53 with the worst
clinical staging, these results were not supported when the patients' outcome were
analyzed. The results of this study indicate that XRCC1 and APE1 participate in the
process of carcinogenesis of OTSCC, but the immunohistochemical expression of
these proteins and also p53 and Ki67 did not show any association with prognostic
parameters. |
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