Azilsartan Increases Levels of IL-10, Down-Regulates MMP-2, MMP-9, RANKL/RANK, Cathepsin K and Up- Regulates OPG in an Experimental Periodontitis Model

Aims The aim of this study was to evaluate the effects of azilsartan (AZT) on bone loss, inflammation, and the expression of matrix metallo proteinases (MMPs), receptor activator of nuclear factor κB ligand (RANKL), receptor activator of nuclear factor κB (RANK), osteoprotegerin (OPG), cyclooxygena...

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Principais autores: Araújo, Aurigena Antunes de, Varela, Hugo, Brito, Gerly Anne de Castro, Medeiros, Caroline Addison Carvalho Xavier de, Araújo, Lorena de Souza, Nascimento, José Heriberto Oliveira do, Araújo Júnior, Raimundo Fernandes de
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Idioma:eng
Publicado em: Faculté de Médecine de Nantes, France
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azilsartan
Endereço do item:https://repositorio.ufrn.br/jspui/handle/123456789/25421
https://doi.org/10.1371/journal.pone.0096750
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spelling ri-123456789-254212021-11-11T19:49:00Z Azilsartan Increases Levels of IL-10, Down-Regulates MMP-2, MMP-9, RANKL/RANK, Cathepsin K and Up- Regulates OPG in an Experimental Periodontitis Model Araújo, Aurigena Antunes de Varela, Hugo Brito, Gerly Anne de Castro Medeiros, Caroline Addison Carvalho Xavier de Araújo, Lorena de Souza Nascimento, José Heriberto Oliveira do Araújo Júnior, Raimundo Fernandes de azilsartan Aims The aim of this study was to evaluate the effects of azilsartan (AZT) on bone loss, inflammation, and the expression of matrix metallo proteinases (MMPs), receptor activator of nuclear factor κB ligand (RANKL), receptor activator of nuclear factor κB (RANK), osteoprotegerin (OPG), cyclooxygenase-2 (COX-2), and cathepsin K in periodontal tissue in a rat model of ligature-induced periodontitis. Materials and Methods Male Wistar albino rats were randomly divided into 5 groups of 10 rats each: (1) nonligated, water; (2) ligated, water; (3) ligated, 1 mg/kg AZT; (4) ligated, 5 mg/kg AZT; and (5) ligated, 10 mg/kg AZT. All groups were treated with saline or AZT for 10 days. Periodontal tissues were analyzed by histopathology and immunohistochemical detection of MMP-2, MMP-9, COX-2, RANKL, RANK, OPG, and cathepsin K. Levels of IL-1β, IL-10, TNF-α, myeloperoxidase (MPO), and glutathione (GSH) were determined by ELISA. Results Treatment with 5 mg/kg AZT resulted in reduced MPO (p<0.05) and IL-1β (p<0.05), increased levels of IL-10 (p<0.05), and reduced expression of MMP-2, MMP-9, COX-2, RANK, RANKL, cathepsin K, and increased expression of OPG. Conclusions These findings reveal that AZT increases anti-inflammatory cytokines and GSH and decreases bone loss in ligature-induced periodontitis in rats. 2018-06-16T11:49:09Z 2018-06-16T11:49:09Z 2014-05-12 article ARAÚJO, Aurigena Antunes de et al. Azilsartan Increases Levels of IL-10, Down-Regulates MMP-2, MMP-9, RANKL/RANK, Cathepsin K and Up-Regulates OPG in an Experimental Periodontitis Model. Plos One, v. 9, p. 01-08, 2014. Disponível em: <http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0096750>. Acesso em: 19 mar. 2018. 1932-6203 https://repositorio.ufrn.br/jspui/handle/123456789/25421 https://doi.org/10.1371/journal.pone.0096750 eng Acesso Aberto application/pdf Faculté de Médecine de Nantes, France
institution Repositório Institucional
collection RI - UFRN
language eng
topic azilsartan
spellingShingle azilsartan
Araújo, Aurigena Antunes de
Varela, Hugo
Brito, Gerly Anne de Castro
Medeiros, Caroline Addison Carvalho Xavier de
Araújo, Lorena de Souza
Nascimento, José Heriberto Oliveira do
Araújo Júnior, Raimundo Fernandes de
Azilsartan Increases Levels of IL-10, Down-Regulates MMP-2, MMP-9, RANKL/RANK, Cathepsin K and Up- Regulates OPG in an Experimental Periodontitis Model
description Aims The aim of this study was to evaluate the effects of azilsartan (AZT) on bone loss, inflammation, and the expression of matrix metallo proteinases (MMPs), receptor activator of nuclear factor κB ligand (RANKL), receptor activator of nuclear factor κB (RANK), osteoprotegerin (OPG), cyclooxygenase-2 (COX-2), and cathepsin K in periodontal tissue in a rat model of ligature-induced periodontitis. Materials and Methods Male Wistar albino rats were randomly divided into 5 groups of 10 rats each: (1) nonligated, water; (2) ligated, water; (3) ligated, 1 mg/kg AZT; (4) ligated, 5 mg/kg AZT; and (5) ligated, 10 mg/kg AZT. All groups were treated with saline or AZT for 10 days. Periodontal tissues were analyzed by histopathology and immunohistochemical detection of MMP-2, MMP-9, COX-2, RANKL, RANK, OPG, and cathepsin K. Levels of IL-1β, IL-10, TNF-α, myeloperoxidase (MPO), and glutathione (GSH) were determined by ELISA. Results Treatment with 5 mg/kg AZT resulted in reduced MPO (p<0.05) and IL-1β (p<0.05), increased levels of IL-10 (p<0.05), and reduced expression of MMP-2, MMP-9, COX-2, RANK, RANKL, cathepsin K, and increased expression of OPG. Conclusions These findings reveal that AZT increases anti-inflammatory cytokines and GSH and decreases bone loss in ligature-induced periodontitis in rats.
format article
author Araújo, Aurigena Antunes de
Varela, Hugo
Brito, Gerly Anne de Castro
Medeiros, Caroline Addison Carvalho Xavier de
Araújo, Lorena de Souza
Nascimento, José Heriberto Oliveira do
Araújo Júnior, Raimundo Fernandes de
author_facet Araújo, Aurigena Antunes de
Varela, Hugo
Brito, Gerly Anne de Castro
Medeiros, Caroline Addison Carvalho Xavier de
Araújo, Lorena de Souza
Nascimento, José Heriberto Oliveira do
Araújo Júnior, Raimundo Fernandes de
author_sort Araújo, Aurigena Antunes de
title Azilsartan Increases Levels of IL-10, Down-Regulates MMP-2, MMP-9, RANKL/RANK, Cathepsin K and Up- Regulates OPG in an Experimental Periodontitis Model
title_short Azilsartan Increases Levels of IL-10, Down-Regulates MMP-2, MMP-9, RANKL/RANK, Cathepsin K and Up- Regulates OPG in an Experimental Periodontitis Model
title_full Azilsartan Increases Levels of IL-10, Down-Regulates MMP-2, MMP-9, RANKL/RANK, Cathepsin K and Up- Regulates OPG in an Experimental Periodontitis Model
title_fullStr Azilsartan Increases Levels of IL-10, Down-Regulates MMP-2, MMP-9, RANKL/RANK, Cathepsin K and Up- Regulates OPG in an Experimental Periodontitis Model
title_full_unstemmed Azilsartan Increases Levels of IL-10, Down-Regulates MMP-2, MMP-9, RANKL/RANK, Cathepsin K and Up- Regulates OPG in an Experimental Periodontitis Model
title_sort azilsartan increases levels of il-10, down-regulates mmp-2, mmp-9, rankl/rank, cathepsin k and up- regulates opg in an experimental periodontitis model
publisher Faculté de Médecine de Nantes, France
publishDate 2018
url https://repositorio.ufrn.br/jspui/handle/123456789/25421
https://doi.org/10.1371/journal.pone.0096750
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