Vesículas extracelulares derivadas de macrófagos alteram o potencial de invasão, proliferação e migração de linhagens celulares do carcinoma de células escamosas de língua oral
Cancer is an entity composed of proliferating malignant cells associated with the different types surrounding cells, forming the tumor microenvironment (TME), where there is a constant exchange of information. One of the ways of communicating between different types of TME cells is through the re...
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| Príomhchruthaitheoir: | |
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| Rannpháirtithe: | |
| Formáid: | doctoralThesis |
| Teanga: | por |
| Foilsithe / Cruthaithe: |
Brasil
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| Ábhair: | |
| Rochtain ar líne: | https://repositorio.ufrn.br/jspui/handle/123456789/25238 |
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| Achoimre: | Cancer is an entity composed of proliferating malignant cells associated with the different
types surrounding cells, forming the tumor microenvironment (TME), where there is a
constant exchange of information. One of the ways of communicating between different types
of TME cells is through the release of extracellular vesicles (EVs), a field of study that
remains poorly understood. The aim of the present study was to evaluate the effects of EVs
released from TME macrophages, which are cells highly plastic in their phenotype (M1
showing an anti-tumor profile and M2 exhibiting a pro-tumor profile) in different cell lines of
tongue squamous cells carcinoma (TSCC) regarding to invasive, proliferative and migratory
capacity. It was observed that EVs samples obtained from macrophages were relatively pure
in EVs, although they were non-specific subtypes. In the myoma invasion assay, it was
observed that when inflammatory cells were co-cultured with HSC-3 cells, M1 cells inhibited
invasion and M2 increased the invasive ability of the malignant cells. On the other hand,
treatment with M1 EVs increased the invasive capacity of HSC-3 cells, and treatment with
M2 EVs inhibited the invasion of these malignant cells, and a similar profile was observed in
SCC-25 and SAS cells when they were submitted to the same treatments. In the analysis of
the Ki-67 marker in myomas, HSC-3, SCC-25 and SAS cells showed the same proliferation
pattern regardless the type of the treatment used when compared to the respective negative
controls. When it was analyzed the proliferation of malignant cells in IncuCyte® treated with
EVs derived from different types of macrophages at different concentrations, an increase in
the proliferative ability of HSC-3 and SAS cells treated with M1 EVs was observed in a dosedependent
pattern. An increase in proliferative ability in dose-dependent profile was also
observed when SAS cells were treated with M2 EVs. In the other proliferation assays
performed in IncuCyte®, effects on proliferative capacity were also highlighted, however a
dose-dependent pattern was not observed. In the IncuCyte® migration assay, significant
differences were observed in the migration capacity of SCC-25 and SAS cells treated with
different types of EVs at different concentrations when compared to the negative control. The
findings of this study suggest that macrophages-derived EVs are pivotal factors in TSCC
tumorigenesis, as well as permits discussions on the different effects of inflammatory cells on
TME depending on the type of cell communication performed. |
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