Imunoexpressão das proteínas twist e e-caderina em carcinoma epidermoide de lábio inferior
Lower lip squamous cell carcinoma (LLSCC) accounts for approximately 30% of all cases of oral cancer, and results mainly from chronic exposure to ultraviolet radiation. E-cadherin and Twist proteins represent biomarkers involved in the epithelial-mesenchymal transition process, a determinant event i...
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Carcinoma de células escamosas Fatores de transcrição Twist Caderinas Prognóstico CNPQ::CIENCIAS DA SAUDE: PATOLOGIA ORAL |
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Carcinoma de células escamosas Fatores de transcrição Twist Caderinas Prognóstico CNPQ::CIENCIAS DA SAUDE: PATOLOGIA ORAL Morais, Everton Freitas de Imunoexpressão das proteínas twist e e-caderina em carcinoma epidermoide de lábio inferior |
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Lower lip squamous cell carcinoma (LLSCC) accounts for approximately 30% of all cases of oral cancer, and results mainly from chronic exposure to ultraviolet radiation. E-cadherin and Twist proteins represent biomarkers involved in the epithelial-mesenchymal transition process, a determinant event in the potential for tumor invasion. The objective of this study was to analyze the immunoexpression pattern of Twist and E-cadherin proteins in LLSCC, investigating possible associations with clinical-pathological parameters indicative of tumor behavior, as well as to evaluate the correlation between proteins. We also evaluated the correlation between Twist and E-cadherin proteins in LLSCC cases. For the analysis of histopathological grading systems of malignancy, the systems proposed by Bryne et al. (1992), Brandwein-Gensler et al. (2005) and Almangush et al. (2014). Increased expression of E-cadherin (general, membrane and cytoplasmic) in LLSCC classified in early clinical stages (stage I and II) (p <0.05) and higher expression of E-cadherin (general and membrane) in cases with remission after 5 years of follow-up (p<0.05). No significant relationships of E-cadherin expression were observed with tumor size, regional metastasis, relapse and the histopathological grade of malignancy. In relation to the Twist protein, it was found a greater general, nuclear and cytoplasmic expression of the same in lesions classified in late stages (III and IV), with recurrence and a high degree of malignancy (p<0.05). In addition, a greater overall expression of Twist protein was also found in high-risk lesions according to the system proposed by Brandwein-Gensler et al. (2005). There was also a significant positive correlation between Twist nuclear immunoexpression and cytoplasmic E-cadherin expression (r = 0.261, p = 0.046). In turn, a significant negative correlation was observed between Twist cytoplasmic expression and E-cadherin membrane expression (r = -0.286; p = 0.028). The results of the present study suggest the potential involvement of Twist and E-cadherin proteins in the modulation of events related to the worst prognosis of LLSCC. |
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Freitas, Roseana de Almeida |
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Freitas, Roseana de Almeida Morais, Everton Freitas de |
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masterThesis |
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Morais, Everton Freitas de |
| author_sort |
Morais, Everton Freitas de |
| title |
Imunoexpressão das proteínas twist e e-caderina em carcinoma epidermoide de lábio inferior |
| title_short |
Imunoexpressão das proteínas twist e e-caderina em carcinoma epidermoide de lábio inferior |
| title_full |
Imunoexpressão das proteínas twist e e-caderina em carcinoma epidermoide de lábio inferior |
| title_fullStr |
Imunoexpressão das proteínas twist e e-caderina em carcinoma epidermoide de lábio inferior |
| title_full_unstemmed |
Imunoexpressão das proteínas twist e e-caderina em carcinoma epidermoide de lábio inferior |
| title_sort |
imunoexpressão das proteínas twist e e-caderina em carcinoma epidermoide de lábio inferior |
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Brasil |
| publishDate |
2018 |
| url |
https://repositorio.ufrn.br/jspui/handle/123456789/25153 |
| work_keys_str_mv |
AT moraisevertonfreitasde imunoexpressaodasproteinastwisteecaderinaemcarcinomaepidermoidedelabioinferior |
| _version_ |
1773966946191015936 |
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ri-123456789-251532019-01-30T06:04:01Z Imunoexpressão das proteínas twist e e-caderina em carcinoma epidermoide de lábio inferior Morais, Everton Freitas de Freitas, Roseana de Almeida Costa, Antonio de Lisboa Lopes Santos, Edilmar de Moura Carcinoma de células escamosas Fatores de transcrição Twist Caderinas Prognóstico CNPQ::CIENCIAS DA SAUDE: PATOLOGIA ORAL Lower lip squamous cell carcinoma (LLSCC) accounts for approximately 30% of all cases of oral cancer, and results mainly from chronic exposure to ultraviolet radiation. E-cadherin and Twist proteins represent biomarkers involved in the epithelial-mesenchymal transition process, a determinant event in the potential for tumor invasion. The objective of this study was to analyze the immunoexpression pattern of Twist and E-cadherin proteins in LLSCC, investigating possible associations with clinical-pathological parameters indicative of tumor behavior, as well as to evaluate the correlation between proteins. We also evaluated the correlation between Twist and E-cadherin proteins in LLSCC cases. For the analysis of histopathological grading systems of malignancy, the systems proposed by Bryne et al. (1992), Brandwein-Gensler et al. (2005) and Almangush et al. (2014). Increased expression of E-cadherin (general, membrane and cytoplasmic) in LLSCC classified in early clinical stages (stage I and II) (p <0.05) and higher expression of E-cadherin (general and membrane) in cases with remission after 5 years of follow-up (p<0.05). No significant relationships of E-cadherin expression were observed with tumor size, regional metastasis, relapse and the histopathological grade of malignancy. In relation to the Twist protein, it was found a greater general, nuclear and cytoplasmic expression of the same in lesions classified in late stages (III and IV), with recurrence and a high degree of malignancy (p<0.05). In addition, a greater overall expression of Twist protein was also found in high-risk lesions according to the system proposed by Brandwein-Gensler et al. (2005). There was also a significant positive correlation between Twist nuclear immunoexpression and cytoplasmic E-cadherin expression (r = 0.261, p = 0.046). In turn, a significant negative correlation was observed between Twist cytoplasmic expression and E-cadherin membrane expression (r = -0.286; p = 0.028). The results of the present study suggest the potential involvement of Twist and E-cadherin proteins in the modulation of events related to the worst prognosis of LLSCC. Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) O carcinoma epidermoide de lábio inferior (CELI) corresponde a aproximadamente 30% de todos os casos de câncer de boca e resulta principalmente da exposição crônica à radiação ultravioleta. As proteínas E-caderina e Twist representam biomarcadores envolvidos no processo de transição epitélio-mesênquima, evento determinante no potencial de invasão tumoral. O objetivo desta pesquisa foi analisar o padrão de imunoexpressão das proteínas Twist e E-caderina em CELI, investigando possíveis associações com parâmetros clínico-patológicos indicadores do comportamento tumoral, além de avaliar a correlação entre as proteínas. Para o estabelecimento da gradação histopatológica de malignidade, foram utilizados os sistemas propostos por Bryne et al. (1992), Brandwein-Gensler et al. (2005) e Almangush et al. (2014). A análise imunoistoquímica revelou maior expressão de E-caderina (geral, membranar e citoplasmática) em CELI classificados em estádios clínicos iniciais (estágio I e II) (p<0,05) e maior expressão de E-caderina (geral e membranar) em casos com sobrevida livre de doença da doença após 5 anos de seguimento (p < 0,05). Não foram observadas associações significativas da expressão de E-caderina com o tamanho do tumor, metástase regional, recidiva e grau histopatológico de malignidade (p>0,05). Em relação à proteína Twist, constatou-se maior expressão geral, nuclear e citoplasmática da mesma em lesões classificadas em estádios tardios (III e IV), com recidiva e de alto grau de malignidade (p<0,05). Além disso, também foi encontrada maior expressão geral da proteína Twist em lesões de alto risco de acordo com o sistema proposto por Brandwein-Gensler et al. (2005). Evidenciou-se, ainda, correlação positiva significativa entre a imunoexpressão nuclear de Twist e a expressão citoplasmática de E-caderina (r= 0,261; p= 0,046). Por sua vez, observou-se correlação negativa significativa entre a expressão citoplasmática de Twist e a expressão membranar de E-caderina (r= -0,286; p= 0,028). Os resultados do presente estudo sugerem o potencial envolvimento das proteínas Twist e E-caderina na modulação de eventos relacionados ao pior prognóstico do CELI. 2018-05-09T21:34:14Z 2018-05-09T21:34:14Z 2018-02-16 masterThesis MORAIS, Everton Freitas de. Imunoexpressão das proteínas twist e e-caderina em carcinoma epidermoide de lábio inferior. 2018. 94f. Dissertação (Mestrado em Patologia Oral) - Centro de Ciências da Saúde, Universidade Federal do Rio Grande do Norte, Natal, 2018. https://repositorio.ufrn.br/jspui/handle/123456789/25153 por Acesso Aberto application/pdf Brasil UFRN PROGRAMA DE PÓS-GRADUAÇÃO EM PATOLOGIA ORAL |