Acute and chronic alcohol administration: effects on performance of zebrafish in a latent learning task

Alcohol abuse is a major medical problem. Zebrafish have been proposed to model alcohol related human disorders. Alcohol impairs learning and memory. Here, we analyze the effects of alcohol on performance of zebrafish in a recently developed latent learning paradigm. We employ a 2 × 3 × 2 experime...

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Principais autores: Luchiari, Ana Carolina, Salajan, Diana C., Gerlai, Robert
Formato: article
Idioma:eng
Publicado em: Elsevier
Assuntos:
Latent learning
Memory
Alcohol
Zebrafish
High-throughput screening
Endereço do item:https://repositorio.ufrn.br/jspui/handle/123456789/24633
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spelling ri-123456789-246332022-12-15T23:31:39Z Acute and chronic alcohol administration: effects on performance of zebrafish in a latent learning task Luchiari, Ana Carolina Salajan, Diana C. Gerlai, Robert Latent learning Memory Alcohol Zebrafish High-throughput screening Alcohol abuse is a major medical problem. Zebrafish have been proposed to model alcohol related human disorders. Alcohol impairs learning and memory. Here, we analyze the effects of alcohol on performance of zebrafish in a recently developed latent learning paradigm. We employ a 2 × 3 × 2 experimental design (chronic × acute alcohol treatment × path blocked). The latent learning task had two phases: one, 30 min long exploration trials (16 days, 1 trial/day) with left or right path of a complex maze blocked, and two, a subsequent probe trial with all paths open leading to a goal box that now contained stimulus fish. During the 16 days each fish received one of two chronic treatments: freshwater or 0.50% (v/v%) alcohol. Subsequently, fish were immersed for 1 h in one of the following solutions: 0.00 (freshwater), 0.50% or 1.00% alcohol, the acute challenge. Behavior of fish was recorded during the probe trial that commenced immediately after the acute treatment. Path choices, latency to leave the start box and to enter the goal box, time spent in the goal box, distance traveled, and duration of freezing were quantified. We found that acute exposure to 1.00% alcohol after chronic freshwater disrupted learning performance, so did exposure to freshwater after chronic alcohol treatment (withdrawal). We also found exposure to chronic alcohol to diminish the effect of subsequent acute alcohol suggesting development of tolerance. Our results demonstrate that analysis of learning performance of zebrafish allows detection of alcoholinduced functional changes. The simplicity and scalability of the employed task also imply the utility of the zebrafish in high throughput drug screens 2018-01-22T19:27:12Z 2018-01-22T19:27:12Z 2015 article LUCHIARI, Ana Carolina; SALAJAN, Diana C.; ROBERT, Gerlai. Acute and chronic alcohol administration: effects on performance of zebrafish in a latent learning task. Behavioural Brain Research, v. 282, p. 76-83, 2015. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0166432814008006?via%3Dihub>. Acesso em: 18 out. 2017. 0166-4328 https://repositorio.ufrn.br/jspui/handle/123456789/24633 10.1016/j.bbr.2014.12.013 eng Acesso Aberto Elsevier
institution Repositório Institucional
collection RI - UFRN
language eng
topic Latent learning
Memory
Alcohol
Zebrafish
High-throughput screening
spellingShingle Latent learning
Memory
Alcohol
Zebrafish
High-throughput screening
Luchiari, Ana Carolina
Salajan, Diana C.
Gerlai, Robert
Acute and chronic alcohol administration: effects on performance of zebrafish in a latent learning task
description Alcohol abuse is a major medical problem. Zebrafish have been proposed to model alcohol related human disorders. Alcohol impairs learning and memory. Here, we analyze the effects of alcohol on performance of zebrafish in a recently developed latent learning paradigm. We employ a 2 × 3 × 2 experimental design (chronic × acute alcohol treatment × path blocked). The latent learning task had two phases: one, 30 min long exploration trials (16 days, 1 trial/day) with left or right path of a complex maze blocked, and two, a subsequent probe trial with all paths open leading to a goal box that now contained stimulus fish. During the 16 days each fish received one of two chronic treatments: freshwater or 0.50% (v/v%) alcohol. Subsequently, fish were immersed for 1 h in one of the following solutions: 0.00 (freshwater), 0.50% or 1.00% alcohol, the acute challenge. Behavior of fish was recorded during the probe trial that commenced immediately after the acute treatment. Path choices, latency to leave the start box and to enter the goal box, time spent in the goal box, distance traveled, and duration of freezing were quantified. We found that acute exposure to 1.00% alcohol after chronic freshwater disrupted learning performance, so did exposure to freshwater after chronic alcohol treatment (withdrawal). We also found exposure to chronic alcohol to diminish the effect of subsequent acute alcohol suggesting development of tolerance. Our results demonstrate that analysis of learning performance of zebrafish allows detection of alcoholinduced functional changes. The simplicity and scalability of the employed task also imply the utility of the zebrafish in high throughput drug screens
format article
author Luchiari, Ana Carolina
Salajan, Diana C.
Gerlai, Robert
author_facet Luchiari, Ana Carolina
Salajan, Diana C.
Gerlai, Robert
author_sort Luchiari, Ana Carolina
title Acute and chronic alcohol administration: effects on performance of zebrafish in a latent learning task
title_short Acute and chronic alcohol administration: effects on performance of zebrafish in a latent learning task
title_full Acute and chronic alcohol administration: effects on performance of zebrafish in a latent learning task
title_fullStr Acute and chronic alcohol administration: effects on performance of zebrafish in a latent learning task
title_full_unstemmed Acute and chronic alcohol administration: effects on performance of zebrafish in a latent learning task
title_sort acute and chronic alcohol administration: effects on performance of zebrafish in a latent learning task
publisher Elsevier
publishDate 2018
url https://repositorio.ufrn.br/jspui/handle/123456789/24633
work_keys_str_mv AT luchiarianacarolina acuteandchronicalcoholadministrationeffectsonperformanceofzebrafishinalatentlearningtask
AT salajandianac acuteandchronicalcoholadministrationeffectsonperformanceofzebrafishinalatentlearningtask
AT gerlairobert acuteandchronicalcoholadministrationeffectsonperformanceofzebrafishinalatentlearningtask
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