Acute and chronic alcohol administration: effects on performance of zebrafish in a latent learning task
Alcohol abuse is a major medical problem. Zebrafish have been proposed to model alcohol related human disorders. Alcohol impairs learning and memory. Here, we analyze the effects of alcohol on performance of zebrafish in a recently developed latent learning paradigm. We employ a 2 × 3 × 2 experime...
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Principais autores: | , , |
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Formato: | article |
Idioma: | eng |
Publicado em: |
Elsevier
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Endereço do item: | https://repositorio.ufrn.br/jspui/handle/123456789/24633 |
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Resumo: | Alcohol abuse is a major medical problem. Zebrafish have been proposed to model alcohol related human
disorders. Alcohol impairs learning and memory. Here, we analyze the effects of alcohol on performance
of zebrafish in a recently developed latent learning paradigm. We employ a 2 × 3 × 2 experimental design
(chronic × acute alcohol treatment × path blocked). The latent learning task had two phases: one, 30 min
long exploration trials (16 days, 1 trial/day) with left or right path of a complex maze blocked, and
two, a subsequent probe trial with all paths open leading to a goal box that now contained stimulus
fish. During the 16 days each fish received one of two chronic treatments: freshwater or 0.50% (v/v%)
alcohol. Subsequently, fish were immersed for 1 h in one of the following solutions: 0.00 (freshwater),
0.50% or 1.00% alcohol, the acute challenge. Behavior of fish was recorded during the probe trial that
commenced immediately after the acute treatment. Path choices, latency to leave the start box and to
enter the goal box, time spent in the goal box, distance traveled, and duration of freezing were quantified.
We found that acute exposure to 1.00% alcohol after chronic freshwater disrupted learning performance,
so did exposure to freshwater after chronic alcohol treatment (withdrawal). We also found exposure to
chronic alcohol to diminish the effect of subsequent acute alcohol suggesting development of tolerance.
Our results demonstrate that analysis of learning performance of zebrafish allows detection of alcoholinduced
functional changes. The simplicity and scalability of the employed task also imply the utility of
the zebrafish in high throughput drug screens |
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