Experience-dependent reactivation of the calcium signal transduction pathway in the rat hippocampus during sleep

Sleep-dependent plastic changes play a key role in the consolidation of newly acquired memories. Two distinct and successive phases of sleep, slow wave sleep (SWS), and rapid eye movement (REM) sleep can be recognized in mammals. Both phases have been implicated in the sensorimotor processing of day...

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Principais autores:	Pereira, C. M., Cota, V. R., Santos, S., Dias, G., Souza, A. C., Ribeiro, Sidarta Tollendal Gomes, Nicolelis, M. A. L.
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Idioma:	eng
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Assuntos:	Memory Sleep Kinase
Endereço do item:	https://repositorio.ufrn.br/jspui/handle/123456789/23972
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spelling	ri-123456789-239722021-07-09T23:11:54Z Experience-dependent reactivation of the calcium signal transduction pathway in the rat hippocampus during sleep Pereira, C. M. Cota, V. R. Santos, S. Dias, G. Souza, A. C. Ribeiro, Sidarta Tollendal Gomes Nicolelis, M. A. L. Memory Sleep Kinase Sleep-dependent plastic changes play a key role in the consolidation of newly acquired memories. Two distinct and successive phases of sleep, slow wave sleep (SWS), and rapid eye movement (REM) sleep can be recognized in mammals. Both phases have been implicated in the sensorimotor processing of daytime events, but the molecular mechanisms involved remain poorly understood. Brain expression of the plasticity-associated immediate-early gene (IEG) zif-268 is upregulated during REM sleep in the cerebral cortex and hippocampus of animals exposed to rich sensorimotor experience in the preceding waking period (Learn Mem. 6; 500, 1999). Zif-268 integrates a major calcium signal transduction pathway which includes Ca(2+)/calmodulin-dependent protein kinase II (CaMKII) and mitogen activated protein kinase (MAPK). CaMKII autophosphorylation of T286 is of special importance because it makes the enzyme active in the absence of Ca(2+), providing a biochemical memory that is critical for plasticity. MAPK, an integral component of cellular signaling during mitotic cell differentiation, has been implicated in hippocampal long-term potentiation (LTP) and learning and memory in behaving animals. Our goal here is to investigate the phosphorylation levels of CaMKII and MAPK during sleep in rats exposed to a new rich environment in the preceding waking period. Intracranial local field potentials (LFPs) recorded in the cortex and hippocampus were used to characterize the wake-sleep cycle (J. Neurosci. 24; 11137, 2004). The phosphorylation levels of CaMKII and MAPK were assessed using specific antibodies for western blots and immunohistochemistry. Our preliminary data (WK n=3, SWS n=5 and REM n=3)) indicate that the MAPK pathway was reactivated in the hippocampus after a few minutes of SWS. Interestingly, for reasons still unknown, MAPK phosphorylation decreased to WK level after a single episode of REM sleep. Our results also showed CaMKII reactivation during REM sleep in the hippocampus of rats previously exposed to novel objects in the preceding WK period. Controls unexposed to novel experience did not show kinase reactivation during sleep. Our results support the notion that sleep harbors experience-dependent mechanisms of synaptic upscaling (Learn Mem. 6; 500, 1999, J. Neurosci. 22; 10914, 2002, J. Neurochem. 95; 418, 2005, Science, 313; 1775, 2006, FINS 1; 43, 2007, Neuron 61; 454, 2009). 2017-09-28T11:51:50Z 2017-09-28T11:51:50Z 2009-10-20 conferenceObject https://repositorio.ufrn.br/jspui/handle/123456789/23972 eng Acesso Aberto application/pdf
institution	Repositório Institucional
collection	RI - UFRN
language	eng
topic	Memory Sleep Kinase
spellingShingle	Memory Sleep Kinase Pereira, C. M. Cota, V. R. Santos, S. Dias, G. Souza, A. C. Ribeiro, Sidarta Tollendal Gomes Nicolelis, M. A. L. Experience-dependent reactivation of the calcium signal transduction pathway in the rat hippocampus during sleep
description	Sleep-dependent plastic changes play a key role in the consolidation of newly acquired memories. Two distinct and successive phases of sleep, slow wave sleep (SWS), and rapid eye movement (REM) sleep can be recognized in mammals. Both phases have been implicated in the sensorimotor processing of daytime events, but the molecular mechanisms involved remain poorly understood. Brain expression of the plasticity-associated immediate-early gene (IEG) zif-268 is upregulated during REM sleep in the cerebral cortex and hippocampus of animals exposed to rich sensorimotor experience in the preceding waking period (Learn Mem. 6; 500, 1999). Zif-268 integrates a major calcium signal transduction pathway which includes Ca(2+)/calmodulin-dependent protein kinase II (CaMKII) and mitogen activated protein kinase (MAPK). CaMKII autophosphorylation of T286 is of special importance because it makes the enzyme active in the absence of Ca(2+), providing a biochemical memory that is critical for plasticity. MAPK, an integral component of cellular signaling during mitotic cell differentiation, has been implicated in hippocampal long-term potentiation (LTP) and learning and memory in behaving animals. Our goal here is to investigate the phosphorylation levels of CaMKII and MAPK during sleep in rats exposed to a new rich environment in the preceding waking period. Intracranial local field potentials (LFPs) recorded in the cortex and hippocampus were used to characterize the wake-sleep cycle (J. Neurosci. 24; 11137, 2004). The phosphorylation levels of CaMKII and MAPK were assessed using specific antibodies for western blots and immunohistochemistry. Our preliminary data (WK n=3, SWS n=5 and REM n=3)) indicate that the MAPK pathway was reactivated in the hippocampus after a few minutes of SWS. Interestingly, for reasons still unknown, MAPK phosphorylation decreased to WK level after a single episode of REM sleep. Our results also showed CaMKII reactivation during REM sleep in the hippocampus of rats previously exposed to novel objects in the preceding WK period. Controls unexposed to novel experience did not show kinase reactivation during sleep. Our results support the notion that sleep harbors experience-dependent mechanisms of synaptic upscaling (Learn Mem. 6; 500, 1999, J. Neurosci. 22; 10914, 2002, J. Neurochem. 95; 418, 2005, Science, 313; 1775, 2006, FINS 1; 43, 2007, Neuron 61; 454, 2009).
format	conferenceObject
author	Pereira, C. M. Cota, V. R. Santos, S. Dias, G. Souza, A. C. Ribeiro, Sidarta Tollendal Gomes Nicolelis, M. A. L.
author_facet	Pereira, C. M. Cota, V. R. Santos, S. Dias, G. Souza, A. C. Ribeiro, Sidarta Tollendal Gomes Nicolelis, M. A. L.
author_sort	Pereira, C. M.
title	Experience-dependent reactivation of the calcium signal transduction pathway in the rat hippocampus during sleep
title_short	Experience-dependent reactivation of the calcium signal transduction pathway in the rat hippocampus during sleep
title_full	Experience-dependent reactivation of the calcium signal transduction pathway in the rat hippocampus during sleep
title_fullStr	Experience-dependent reactivation of the calcium signal transduction pathway in the rat hippocampus during sleep
title_full_unstemmed	Experience-dependent reactivation of the calcium signal transduction pathway in the rat hippocampus during sleep
title_sort	experience-dependent reactivation of the calcium signal transduction pathway in the rat hippocampus during sleep
publishDate	2017
url	https://repositorio.ufrn.br/jspui/handle/123456789/23972
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Experience-dependent reactivation of the calcium signal transduction pathway in the rat hippocampus during sleep

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