Detection of HPV DNA and immunohistochemical expression of cell cycle proteins in oral carcinoma in a population of brazilian patients

This study investigated the presence of human papillomavirus (HPV) DNA and viral types in 33 cases of oral squamous cells carcinoma (OSCC) and compared the immunohistochemical expression of the cell-cycle markers p21 and pRb between cases of the disease with and without HPV. DNA was extracted from p...

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Principais autores: Soares, Rosilene Calazans, Oliveira, Márcio Campos de, Souza, Lélia Batista de, Costa, Antonio de Lisboa Lopes, Pinto, Leão Pereira
Formato: article
Idioma:eng
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Assuntos:
DNA Probes
HPV
Carcinoma
Squamous cell cancer
Polymerase Chain Reaction
Hybridization
Cell cycle
Endereço do item:https://repositorio.ufrn.br/jspui/handle/123456789/23862
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spelling ri-123456789-238622021-12-09T16:10:39Z Detection of HPV DNA and immunohistochemical expression of cell cycle proteins in oral carcinoma in a population of brazilian patients Soares, Rosilene Calazans Oliveira, Márcio Campos de Souza, Lélia Batista de Costa, Antonio de Lisboa Lopes Pinto, Leão Pereira DNA Probes HPV Carcinoma Squamous cell cancer Polymerase Chain Reaction Hybridization Cell cycle This study investigated the presence of human papillomavirus (HPV) DNA and viral types in 33 cases of oral squamous cells carcinoma (OSCC) and compared the immunohistochemical expression of the cell-cycle markers p21 and pRb between cases of the disease with and without HPV. DNA was extracted from paraffin-embedded tissue and amplified by PCR for the detection of HPV DNA. Viral typing was performed by dot blot hybridization. Immunohistochemistry was performed by the streptavidin-biotin technique. HPV DNA was detected in 11 (33.33%) of the 33 cases. The prevalent viral type was HPV 18 (81.81%). A significant association was observed between the presence of HPV and immunohistochemical expression of pRb, but not between p21 expression and the presence of the virus. The low frequency of detection of HPV DNA in OSCC suggests a possible participation of the virus in the development and progression of only a subgroup of these tumors. 2017-09-13T15:25:53Z 2017-09-13T15:25:53Z 2008 article SOARES, Rosilene Calazans et al . Detection of HPV DNA and immunohistochemical expression of cell cycle proteins in oral carcinoma in a population of brazilian patients. Journal of Applied Oral Science, v. 16, n. 5, p. 340-344, 2008. https://repositorio.ufrn.br/jspui/handle/123456789/23862 10.1590/S1678-77572008000500007 eng Acesso Aberto application/pdf
institution Repositório Institucional
collection RI - UFRN
language eng
topic DNA Probes
HPV
Carcinoma
Squamous cell cancer
Polymerase Chain Reaction
Hybridization
Cell cycle
spellingShingle DNA Probes
HPV
Carcinoma
Squamous cell cancer
Polymerase Chain Reaction
Hybridization
Cell cycle
Soares, Rosilene Calazans
Oliveira, Márcio Campos de
Souza, Lélia Batista de
Costa, Antonio de Lisboa Lopes
Pinto, Leão Pereira
Detection of HPV DNA and immunohistochemical expression of cell cycle proteins in oral carcinoma in a population of brazilian patients
description This study investigated the presence of human papillomavirus (HPV) DNA and viral types in 33 cases of oral squamous cells carcinoma (OSCC) and compared the immunohistochemical expression of the cell-cycle markers p21 and pRb between cases of the disease with and without HPV. DNA was extracted from paraffin-embedded tissue and amplified by PCR for the detection of HPV DNA. Viral typing was performed by dot blot hybridization. Immunohistochemistry was performed by the streptavidin-biotin technique. HPV DNA was detected in 11 (33.33%) of the 33 cases. The prevalent viral type was HPV 18 (81.81%). A significant association was observed between the presence of HPV and immunohistochemical expression of pRb, but not between p21 expression and the presence of the virus. The low frequency of detection of HPV DNA in OSCC suggests a possible participation of the virus in the development and progression of only a subgroup of these tumors.
format article
author Soares, Rosilene Calazans
Oliveira, Márcio Campos de
Souza, Lélia Batista de
Costa, Antonio de Lisboa Lopes
Pinto, Leão Pereira
author_facet Soares, Rosilene Calazans
Oliveira, Márcio Campos de
Souza, Lélia Batista de
Costa, Antonio de Lisboa Lopes
Pinto, Leão Pereira
author_sort Soares, Rosilene Calazans
title Detection of HPV DNA and immunohistochemical expression of cell cycle proteins in oral carcinoma in a population of brazilian patients
title_short Detection of HPV DNA and immunohistochemical expression of cell cycle proteins in oral carcinoma in a population of brazilian patients
title_full Detection of HPV DNA and immunohistochemical expression of cell cycle proteins in oral carcinoma in a population of brazilian patients
title_fullStr Detection of HPV DNA and immunohistochemical expression of cell cycle proteins in oral carcinoma in a population of brazilian patients
title_full_unstemmed Detection of HPV DNA and immunohistochemical expression of cell cycle proteins in oral carcinoma in a population of brazilian patients
title_sort detection of hpv dna and immunohistochemical expression of cell cycle proteins in oral carcinoma in a population of brazilian patients
publishDate 2017
url https://repositorio.ufrn.br/jspui/handle/123456789/23862
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