High-risk human papillomavirus (HPV) is not associated with p53 and bcl-2 expression in oral squamous cell carcinomas

Objective To determine the frequency and type of human papillomavirus (HPV) in oral squamous cell carcinomas (OSCC), as well as to identify a possible association between HPV infection and the expression pattern of p53 and bcl-2, and identify whether the oral HPV infection is a characteristic fin...

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Principais autores: Oliveira, Márcio Campos, Soares, Rosilene Calazans, Pinto, Leão Pereira, Souza, Lélia Batista de, Medeiros, Sílvia Regina Batistuzzo de, Costa, Antonio de Lisboa Lopes
Formato: article
Idioma:eng
Publicado em: ELSEVIER
Assuntos:
HPV
Tumor suppressor proteins
Oncoproteins
PCR
Hybridization
Immunohistochemistry
Endereço do item:https://repositorio.ufrn.br/jspui/handle/123456789/23816
https://doi.org/10.1016/j.anl.2008.10.011
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spelling ri-123456789-238162022-12-15T22:00:24Z High-risk human papillomavirus (HPV) is not associated with p53 and bcl-2 expression in oral squamous cell carcinomas Oliveira, Márcio Campos Soares, Rosilene Calazans Pinto, Leão Pereira Souza, Lélia Batista de Medeiros, Sílvia Regina Batistuzzo de Costa, Antonio de Lisboa Lopes HPV Tumor suppressor proteins Oncoproteins PCR Hybridization Immunohistochemistry Objective To determine the frequency and type of human papillomavirus (HPV) in oral squamous cell carcinomas (OSCC), as well as to identify a possible association between HPV infection and the expression pattern of p53 and bcl-2, and identify whether the oral HPV infection is a characteristic finding in our sample. Methods We performed polymerase chain reaction and dot blot hybridization for the detection of HPV DNA in paraffin sections as well as immunohistochemical analysis of p53 and bcl-2 in our sample. Results Twenty-six cases (29.5%) were positive for the virus by PCR. Dot blot hybridization identified HPV 18 in 21 (80.8%) cases, HPV 16 in one (3.8%) case and a combination of the two types in the four (15.4%) remaining cases. No other type of HPV was detected in the sample. Immunohistochemistry showed p53 in 26 (60.4%) cases and bcl-2 in 17 (39.5%) ones. No significant association was observed between the presence of HPV and the expression of the proteins studied (p = 0.988 and p = 0.748, respectively). Conclusions Although this investigation have detected only 29.5% of HR-HPV DNA in OSCC, it is possible that this virus contribute to the development of some case of this tumor. Furthermore, it seems that the immunohistochemical expression of p53 and bcl-2 and the presence of HPV DNA are independent events in OSCC. 2017-09-11T14:49:37Z 2017-09-11T14:49:37Z 2009 article OLIVEIRA, Márcio Campos et al. High-risk human papillomavirus (HPV) is not associated with p53 and bcl-2 expression in oral squamous cell carcinomas. Auris Nasus Larynx, v. 36, n. 4, p. 450-456, 2009. https://repositorio.ufrn.br/jspui/handle/123456789/23816 https://doi.org/10.1016/j.anl.2008.10.011 eng Acesso Aberto ELSEVIER
institution Repositório Institucional
collection RI - UFRN
language eng
topic HPV
Tumor suppressor proteins
Oncoproteins
PCR
Hybridization
Immunohistochemistry
spellingShingle HPV
Tumor suppressor proteins
Oncoproteins
PCR
Hybridization
Immunohistochemistry
Oliveira, Márcio Campos
Soares, Rosilene Calazans
Pinto, Leão Pereira
Souza, Lélia Batista de
Medeiros, Sílvia Regina Batistuzzo de
Costa, Antonio de Lisboa Lopes
High-risk human papillomavirus (HPV) is not associated with p53 and bcl-2 expression in oral squamous cell carcinomas
description Objective To determine the frequency and type of human papillomavirus (HPV) in oral squamous cell carcinomas (OSCC), as well as to identify a possible association between HPV infection and the expression pattern of p53 and bcl-2, and identify whether the oral HPV infection is a characteristic finding in our sample. Methods We performed polymerase chain reaction and dot blot hybridization for the detection of HPV DNA in paraffin sections as well as immunohistochemical analysis of p53 and bcl-2 in our sample. Results Twenty-six cases (29.5%) were positive for the virus by PCR. Dot blot hybridization identified HPV 18 in 21 (80.8%) cases, HPV 16 in one (3.8%) case and a combination of the two types in the four (15.4%) remaining cases. No other type of HPV was detected in the sample. Immunohistochemistry showed p53 in 26 (60.4%) cases and bcl-2 in 17 (39.5%) ones. No significant association was observed between the presence of HPV and the expression of the proteins studied (p = 0.988 and p = 0.748, respectively). Conclusions Although this investigation have detected only 29.5% of HR-HPV DNA in OSCC, it is possible that this virus contribute to the development of some case of this tumor. Furthermore, it seems that the immunohistochemical expression of p53 and bcl-2 and the presence of HPV DNA are independent events in OSCC.
format article
author Oliveira, Márcio Campos
Soares, Rosilene Calazans
Pinto, Leão Pereira
Souza, Lélia Batista de
Medeiros, Sílvia Regina Batistuzzo de
Costa, Antonio de Lisboa Lopes
author_facet Oliveira, Márcio Campos
Soares, Rosilene Calazans
Pinto, Leão Pereira
Souza, Lélia Batista de
Medeiros, Sílvia Regina Batistuzzo de
Costa, Antonio de Lisboa Lopes
author_sort Oliveira, Márcio Campos
title High-risk human papillomavirus (HPV) is not associated with p53 and bcl-2 expression in oral squamous cell carcinomas
title_short High-risk human papillomavirus (HPV) is not associated with p53 and bcl-2 expression in oral squamous cell carcinomas
title_full High-risk human papillomavirus (HPV) is not associated with p53 and bcl-2 expression in oral squamous cell carcinomas
title_fullStr High-risk human papillomavirus (HPV) is not associated with p53 and bcl-2 expression in oral squamous cell carcinomas
title_full_unstemmed High-risk human papillomavirus (HPV) is not associated with p53 and bcl-2 expression in oral squamous cell carcinomas
title_sort high-risk human papillomavirus (hpv) is not associated with p53 and bcl-2 expression in oral squamous cell carcinomas
publisher ELSEVIER
publishDate 2017
url https://repositorio.ufrn.br/jspui/handle/123456789/23816
https://doi.org/10.1016/j.anl.2008.10.011
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