O clareamento dental com peróxido de hidrogênio a 38% induz inflamação moderada e expressão de IL-1 beta, TNF beta, FGF2, GPX e osteocalcina na polpa de ratos
The presence of inflammatory infiltrate in pulp after dental bleaching has been increasingly discussed, however, it is not known which mediators participate in this process. This study analyzed the intensity of inflammatory infiltrate and expression of interleukin (IL)-1β, tumor necrosis factor (TNF...
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| Formato: | Dissertação |
| Idioma: | por |
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Brasil
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| Endereço do item: | https://repositorio.ufrn.br/jspui/handle/123456789/23208 |
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| Resumo: | The presence of inflammatory infiltrate in pulp after dental bleaching has been increasingly discussed, however, it is not known which mediators participate in this process. This study analyzed the intensity of inflammatory infiltrate and expression of interleukin (IL)-1β, tumor necrosis factor (TNF) β, cyclooxygenase (COX)-2, macrophage migration inhibitory factor (MIF), fibroblast growth factor (FGF)2, superoxide dismutase (SOD), glutathione peroxidase (GPX), inducible nitric oxide synthase (iNOS) and osteocalcin, due to bleaching procedure in teeth of rats with 38% hydrogen peroxide gel. Forty incisors of male Wistar rats were bleached, and forty unbleached incisors were used as controls. Two bleaching sessions were performed in two test groups, with an interval of 7 days between them. At each session, animals were anesthetized, and two bleaching gel applications of 15 min each were made. Half of animals were sacrificed after 24 h of last session, and the other half after 10 days. Teeth were processed for histological analysis, confocal immunofluorescence of osteocalcin and immunohistochemistry of IL-1β, TNFβ, COX-2, MIF, FGF2, SOD, GPX and iNOS. The bleached groups presented significant moderate inflammation compared to control groups, which did not present alterations (p <0.05), however, there was no significant difference between the bleached groups with regard to pulp tissue condition and inflammation stage, despite the interval of 10 days for analysis. There was a positive immunoreaction only in bleached groups for IL-1β, TNFβ, FGF2 and GPX. There was no expression of SOD, MIF, COX-2 and iNOS in any groups. The bleached group 10 days showed a statistically superior value of osteocalcin when compared to groups bleached 24 h and control 10 days (p <0.05). In conclusion, tooth bleaching in rats incisors with 38% hydrogen peroxide causes moderate pulp inflammation, and IL-1β, TNFβ, FGF2, GPX and osteocalcin act as mediators in this process. |
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