Behavioral and EEG effects of GABAergic manipulation of the nigro-tectal pathway in the Wistar audiogenic rat (WAR) strain II: An EEG wavelet analysis and retrograde neuronal tracer approach

The role of the substantia nigra pars reticulata (SNPr) and superior colliculus (SC) network in rat strains susceptible to audiogenic seizures still remain underexplored in epileptology. In a previous study from our laboratory, the GABAergic drugs bicuculline (BIC) and muscimol (MUS) were microinj...

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Principais autores: Rossetti, Franco, Rodrigues, Marcelo Cairrão Araújo, Marroni, Simone S., Fernandes, Artur, Foresti, Maira Licia, Romcy-Pereira, Rodrigo Neves, Araújo, Dráulio Barros de, Garcia-Cairasco, Norberto
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Idioma:eng
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Audiogenic seizures
Sustained EEG oscillation
Bicuculline
Muscimol
Fluorogold
Endereço do item:https://repositorio.ufrn.br/jspui/handle/123456789/23174
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spelling ri-123456789-231742021-07-09T22:31:33Z Behavioral and EEG effects of GABAergic manipulation of the nigro-tectal pathway in the Wistar audiogenic rat (WAR) strain II: An EEG wavelet analysis and retrograde neuronal tracer approach Rossetti, Franco Rodrigues, Marcelo Cairrão Araújo Marroni, Simone S. Fernandes, Artur Foresti, Maira Licia Romcy-Pereira, Rodrigo Neves Araújo, Dráulio Barros de Garcia-Cairasco, Norberto Audiogenic seizures Sustained EEG oscillation Bicuculline Muscimol Fluorogold The role of the substantia nigra pars reticulata (SNPr) and superior colliculus (SC) network in rat strains susceptible to audiogenic seizures still remain underexplored in epileptology. In a previous study from our laboratory, the GABAergic drugs bicuculline (BIC) and muscimol (MUS) were microinjected into the deep layers of either the anterior SC (aSC) or the posterior SC (pSC) in animals of the Wistar audiogenic rat (WAR) strain submitted to acoustic stimulation, in which simultaneous electroencephalographic (EEG) recording of the aSC, pSC, SNPr and striatum was performed. Only MUS microinjected into the pSC blocked audiogenic seizures. In the present study, we expanded upon these previous results using the retrograde tracer Fluorogold (FG) microinjected into the aSC and pSC in conjunction with quantitative EEG analysis (wavelet transform), in the search for mechanisms associated with the susceptibility of this inbred strain to acoustic stimulation. Our hypothesis was that the WAR strain would have different connectivity between specific subareas of the superior colliculus and the SNPr when compared with resistant Wistar animals and that these connections would lead to altered behavior of this network during audiogenic seizures. Wavelet analysis showed that the only treatment with an anticonvulsant effect was MUS microinjected into the pSC region, and this treatment induced a sustained oscillation in the theta band only in the SNPr and in the pSC. These data suggest that in WAR animals, there are at least two subcortical loops and that the one involved in audiogenic seizure susceptibility appears to be the pSC-SNPr circuit. We also found that WARs presented an increase in the number of FG+projections from the posterior SNPr to both the aSC and pSC (primarily to the pSC), with both acting as proconvulsant nuclei when compared with Wistar rats. We concluded that these two different subcortical loops within the basal ganglia are probably a consequence of the WAR genetic background. 2017-05-29T14:11:26Z 2017-05-29T14:11:26Z 2012-06-16 article 1525-5050 https://repositorio.ufrn.br/jspui/handle/123456789/23174 eng Acesso Aberto application/pdf
institution Repositório Institucional
collection RI - UFRN
language eng
topic Audiogenic seizures
Sustained EEG oscillation
Bicuculline
Muscimol
Fluorogold
spellingShingle Audiogenic seizures
Sustained EEG oscillation
Bicuculline
Muscimol
Fluorogold
Rossetti, Franco
Rodrigues, Marcelo Cairrão Araújo
Marroni, Simone S.
Fernandes, Artur
Foresti, Maira Licia
Romcy-Pereira, Rodrigo Neves
Araújo, Dráulio Barros de
Garcia-Cairasco, Norberto
Behavioral and EEG effects of GABAergic manipulation of the nigro-tectal pathway in the Wistar audiogenic rat (WAR) strain II: An EEG wavelet analysis and retrograde neuronal tracer approach
description The role of the substantia nigra pars reticulata (SNPr) and superior colliculus (SC) network in rat strains susceptible to audiogenic seizures still remain underexplored in epileptology. In a previous study from our laboratory, the GABAergic drugs bicuculline (BIC) and muscimol (MUS) were microinjected into the deep layers of either the anterior SC (aSC) or the posterior SC (pSC) in animals of the Wistar audiogenic rat (WAR) strain submitted to acoustic stimulation, in which simultaneous electroencephalographic (EEG) recording of the aSC, pSC, SNPr and striatum was performed. Only MUS microinjected into the pSC blocked audiogenic seizures. In the present study, we expanded upon these previous results using the retrograde tracer Fluorogold (FG) microinjected into the aSC and pSC in conjunction with quantitative EEG analysis (wavelet transform), in the search for mechanisms associated with the susceptibility of this inbred strain to acoustic stimulation. Our hypothesis was that the WAR strain would have different connectivity between specific subareas of the superior colliculus and the SNPr when compared with resistant Wistar animals and that these connections would lead to altered behavior of this network during audiogenic seizures. Wavelet analysis showed that the only treatment with an anticonvulsant effect was MUS microinjected into the pSC region, and this treatment induced a sustained oscillation in the theta band only in the SNPr and in the pSC. These data suggest that in WAR animals, there are at least two subcortical loops and that the one involved in audiogenic seizure susceptibility appears to be the pSC-SNPr circuit. We also found that WARs presented an increase in the number of FG+projections from the posterior SNPr to both the aSC and pSC (primarily to the pSC), with both acting as proconvulsant nuclei when compared with Wistar rats. We concluded that these two different subcortical loops within the basal ganglia are probably a consequence of the WAR genetic background.
format article
author Rossetti, Franco
Rodrigues, Marcelo Cairrão Araújo
Marroni, Simone S.
Fernandes, Artur
Foresti, Maira Licia
Romcy-Pereira, Rodrigo Neves
Araújo, Dráulio Barros de
Garcia-Cairasco, Norberto
author_facet Rossetti, Franco
Rodrigues, Marcelo Cairrão Araújo
Marroni, Simone S.
Fernandes, Artur
Foresti, Maira Licia
Romcy-Pereira, Rodrigo Neves
Araújo, Dráulio Barros de
Garcia-Cairasco, Norberto
author_sort Rossetti, Franco
title Behavioral and EEG effects of GABAergic manipulation of the nigro-tectal pathway in the Wistar audiogenic rat (WAR) strain II: An EEG wavelet analysis and retrograde neuronal tracer approach
title_short Behavioral and EEG effects of GABAergic manipulation of the nigro-tectal pathway in the Wistar audiogenic rat (WAR) strain II: An EEG wavelet analysis and retrograde neuronal tracer approach
title_full Behavioral and EEG effects of GABAergic manipulation of the nigro-tectal pathway in the Wistar audiogenic rat (WAR) strain II: An EEG wavelet analysis and retrograde neuronal tracer approach
title_fullStr Behavioral and EEG effects of GABAergic manipulation of the nigro-tectal pathway in the Wistar audiogenic rat (WAR) strain II: An EEG wavelet analysis and retrograde neuronal tracer approach
title_full_unstemmed Behavioral and EEG effects of GABAergic manipulation of the nigro-tectal pathway in the Wistar audiogenic rat (WAR) strain II: An EEG wavelet analysis and retrograde neuronal tracer approach
title_sort behavioral and eeg effects of gabaergic manipulation of the nigro-tectal pathway in the wistar audiogenic rat (war) strain ii: an eeg wavelet analysis and retrograde neuronal tracer approach
publishDate 2017
url https://repositorio.ufrn.br/jspui/handle/123456789/23174
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