Monitoring cell-cell contacts in vivo in transgenic animals

We used a synthetic genetic system based on ligand-induced intramembrane proteolysis to monitor cell-cell contacts in animals. Upon ligand-receptor interaction in sites of cell-cell contact, the transmembrane domain of an engineered receptor is cleaved by intramembrane proteolysis and releases a pro...

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Principais autores:	Huang, Ting-Hao, Velho, Tarciso André Ferreira, Lois, Carlos
Formato:	article
Idioma:	eng
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Assuntos:	Interacting cells Development Adhesion Morphogenesis Cell communication
Endereço do item:	https://repositorio.ufrn.br/jspui/handle/123456789/23071
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id	ri-123456789-23071
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spelling	ri-123456789-230712021-07-12T18:40:57Z Monitoring cell-cell contacts in vivo in transgenic animals Huang, Ting-Hao Velho, Tarciso André Ferreira Lois, Carlos Interacting cells Development Adhesion Morphogenesis Cell communication We used a synthetic genetic system based on ligand-induced intramembrane proteolysis to monitor cell-cell contacts in animals. Upon ligand-receptor interaction in sites of cell-cell contact, the transmembrane domain of an engineered receptor is cleaved by intramembrane proteolysis and releases a protein fragment that regulates transcription in the interacting partners. We demonstrate that the system can be used to regulate gene expression between interacting cells, both in vitro and in vivo, in transgenic Drosophila We show that the system allows for detection of interactions between neurons and glia in the Drosophila nervous system. In addition, we observed that when the ligand is expressed in subsets of neurons with a restricted localization in the brain it leads to activation of transcription in a selected set of glial cells that interact with those neurons. This system will be useful to monitor cell-cell interactions in animals, and can be used to genetically manipulate cells that interact with one another. 2017-05-25T11:53:25Z 2017-05-25T11:53:25Z 2016 article https://repositorio.ufrn.br/jspui/handle/123456789/23071 10.1242/dev.142406 eng Acesso Aberto application/pdf
institution	Repositório Institucional
collection	RI - UFRN
language	eng
topic	Interacting cells Development Adhesion Morphogenesis Cell communication
spellingShingle	Interacting cells Development Adhesion Morphogenesis Cell communication Huang, Ting-Hao Velho, Tarciso André Ferreira Lois, Carlos Monitoring cell-cell contacts in vivo in transgenic animals
description	We used a synthetic genetic system based on ligand-induced intramembrane proteolysis to monitor cell-cell contacts in animals. Upon ligand-receptor interaction in sites of cell-cell contact, the transmembrane domain of an engineered receptor is cleaved by intramembrane proteolysis and releases a protein fragment that regulates transcription in the interacting partners. We demonstrate that the system can be used to regulate gene expression between interacting cells, both in vitro and in vivo, in transgenic Drosophila We show that the system allows for detection of interactions between neurons and glia in the Drosophila nervous system. In addition, we observed that when the ligand is expressed in subsets of neurons with a restricted localization in the brain it leads to activation of transcription in a selected set of glial cells that interact with those neurons. This system will be useful to monitor cell-cell interactions in animals, and can be used to genetically manipulate cells that interact with one another.
format	article
author	Huang, Ting-Hao Velho, Tarciso André Ferreira Lois, Carlos
author_facet	Huang, Ting-Hao Velho, Tarciso André Ferreira Lois, Carlos
author_sort	Huang, Ting-Hao
title	Monitoring cell-cell contacts in vivo in transgenic animals
title_short	Monitoring cell-cell contacts in vivo in transgenic animals
title_full	Monitoring cell-cell contacts in vivo in transgenic animals
title_fullStr	Monitoring cell-cell contacts in vivo in transgenic animals
title_full_unstemmed	Monitoring cell-cell contacts in vivo in transgenic animals
title_sort	monitoring cell-cell contacts in vivo in transgenic animals
publishDate	2017
url	https://repositorio.ufrn.br/jspui/handle/123456789/23071
work_keys_str_mv	AT huangtinghao monitoringcellcellcontactsinvivointransgenicanimals AT velhotarcisoandreferreira monitoringcellcellcontactsinvivointransgenicanimals AT loiscarlos monitoringcellcellcontactsinvivointransgenicanimals
_version_	1773966849226047488

Monitoring cell-cell contacts in vivo in transgenic animals

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