In vitro antibacterial activity of two plant extracts against enterococcus faecalis

The antimicrobial activity of two crude plant extracts– Solanum paniculatum L. (jurubeba) and Bixa orellana L. (annatto) against Enterococcus faecalis was tested in vitro, and their efficacy was compared with that of a chemical agent with known antibacterial activity – chlorhexidine digluconate at 0...

ver descrição completa

Na minha lista:
Detalhes bibliográficos
Principais autores: Duailibe, Silvana A. C., Costa, Maria Regina Macedo, Lima, Kenio Costa de, Uzeda, Milton
Formato: article
Idioma:eng
Publicado em:
Assuntos:
Antimicrobial activity
Plant extracts
Phytotherapeutics
Enterococcus faecalis
Endodontic infections
Bixa orellana L.
Solanum paniculatum L.
Endereço do item:https://repositorio.ufrn.br/jspui/handle/123456789/22929
Tags: Adicionar Tag
Sem tags, seja o primeiro a adicionar uma tag!
id ri-123456789-22929
record_format dspace
spelling ri-123456789-229292021-12-14T17:10:45Z In vitro antibacterial activity of two plant extracts against enterococcus faecalis Duailibe, Silvana A. C. Costa, Maria Regina Macedo Lima, Kenio Costa de Uzeda, Milton Antimicrobial activity Plant extracts Phytotherapeutics Enterococcus faecalis Endodontic infections Bixa orellana L. Solanum paniculatum L. The antimicrobial activity of two crude plant extracts– Solanum paniculatum L. (jurubeba) and Bixa orellana L. (annatto) against Enterococcus faecalis was tested in vitro, and their efficacy was compared with that of a chemical agent with known antibacterial activity – chlorhexidine digluconate at 0.12% concentration. Extracts were prepared from jurubeba roots and annatto seeds after selection by phytochemical screening. Microorganisms were divided into two groups: E. faecalis (ATCC 292012) and E. faecalis (44 - AB). The minimum inhibitory concentration (MIC) and the minimum inhibitory concentration of adherence (MICA) of the extract and the control were determined, and the cytotoxic potential and lethal dose (LD) of jurubeba extract were assessed because it was the only extract to exhibit inhibitory activity against bacteria. Only the S. paniculatum Linn (jurubeba) extract exhibited activity against the two bacterial strains– MIC: up to 1:64 against E. faecalis ATCC 292012, up to 1:32 against E. faecalis of the oral environment (44-AB); MICA: up to 1:512 against the two strains, exhibiting cytotoxicity at the 1:2 dilution (250 mg/mL) and LD = 0. The B. orellana Linn extract exhibited no inhibitory activity in any of the experiments; thus, it was not assayed in the cytotoxicity experiments, nor was its LD determined. The low toxicity of S. paniculatum L. extract (cytotoxicity only at the 1:2 dilution, or 250 mg/mL) in addition to its LD = 0 and good antibacterial performance in the tests suggest the potential use of this product for the treatment of endodontic and periodontal oral infections; however, further experiments strengthening these results should be performed. The B. orellana L. extract exhibited no antimicrobial activity. 2017-05-16T12:17:08Z 2017-05-16T12:17:08Z 2010 article DUAILIBE, Silvana A. C. et al. In vitro antibacterial activity of two plant extracts against enterococcus faecalis. African Journal of Microbiology Research, v. 9, n. 14, p. 1020-1025, 2015. https://repositorio.ufrn.br/jspui/handle/123456789/22929 eng Acesso Aberto application/pdf
institution Repositório Institucional
collection RI - UFRN
language eng
topic Antimicrobial activity
Plant extracts
Phytotherapeutics
Enterococcus faecalis
Endodontic infections
Bixa orellana L.
Solanum paniculatum L.
spellingShingle Antimicrobial activity
Plant extracts
Phytotherapeutics
Enterococcus faecalis
Endodontic infections
Bixa orellana L.
Solanum paniculatum L.
Duailibe, Silvana A. C.
Costa, Maria Regina Macedo
Lima, Kenio Costa de
Uzeda, Milton
In vitro antibacterial activity of two plant extracts against enterococcus faecalis
description The antimicrobial activity of two crude plant extracts– Solanum paniculatum L. (jurubeba) and Bixa orellana L. (annatto) against Enterococcus faecalis was tested in vitro, and their efficacy was compared with that of a chemical agent with known antibacterial activity – chlorhexidine digluconate at 0.12% concentration. Extracts were prepared from jurubeba roots and annatto seeds after selection by phytochemical screening. Microorganisms were divided into two groups: E. faecalis (ATCC 292012) and E. faecalis (44 - AB). The minimum inhibitory concentration (MIC) and the minimum inhibitory concentration of adherence (MICA) of the extract and the control were determined, and the cytotoxic potential and lethal dose (LD) of jurubeba extract were assessed because it was the only extract to exhibit inhibitory activity against bacteria. Only the S. paniculatum Linn (jurubeba) extract exhibited activity against the two bacterial strains– MIC: up to 1:64 against E. faecalis ATCC 292012, up to 1:32 against E. faecalis of the oral environment (44-AB); MICA: up to 1:512 against the two strains, exhibiting cytotoxicity at the 1:2 dilution (250 mg/mL) and LD = 0. The B. orellana Linn extract exhibited no inhibitory activity in any of the experiments; thus, it was not assayed in the cytotoxicity experiments, nor was its LD determined. The low toxicity of S. paniculatum L. extract (cytotoxicity only at the 1:2 dilution, or 250 mg/mL) in addition to its LD = 0 and good antibacterial performance in the tests suggest the potential use of this product for the treatment of endodontic and periodontal oral infections; however, further experiments strengthening these results should be performed. The B. orellana L. extract exhibited no antimicrobial activity.
format article
author Duailibe, Silvana A. C.
Costa, Maria Regina Macedo
Lima, Kenio Costa de
Uzeda, Milton
author_facet Duailibe, Silvana A. C.
Costa, Maria Regina Macedo
Lima, Kenio Costa de
Uzeda, Milton
author_sort Duailibe, Silvana A. C.
title In vitro antibacterial activity of two plant extracts against enterococcus faecalis
title_short In vitro antibacterial activity of two plant extracts against enterococcus faecalis
title_full In vitro antibacterial activity of two plant extracts against enterococcus faecalis
title_fullStr In vitro antibacterial activity of two plant extracts against enterococcus faecalis
title_full_unstemmed In vitro antibacterial activity of two plant extracts against enterococcus faecalis
title_sort in vitro antibacterial activity of two plant extracts against enterococcus faecalis
publishDate 2017
url https://repositorio.ufrn.br/jspui/handle/123456789/22929
work_keys_str_mv AT duailibesilvanaac invitroantibacterialactivityoftwoplantextractsagainstenterococcusfaecalis
AT costamariareginamacedo invitroantibacterialactivityoftwoplantextractsagainstenterococcusfaecalis
AT limakeniocostade invitroantibacterialactivityoftwoplantextractsagainstenterococcusfaecalis
AT uzedamilton invitroantibacterialactivityoftwoplantextractsagainstenterococcusfaecalis
_version_ 1773963841551466496

Registros relacionados