Immunohistochemical expression of protein 53, murine double minute 2, B-cell lymphoma 2, and proliferating cell nuclear antigen in odontogenic cysts and keratocystic odontogenic tumor
INTRODUCTION: Even though odontogenic cysts share a similar histogenesis, they show different growth and differentiation profile due to differences in the proliferative cellular activity. AIMS: We perform an immunohistochemical assessment of protein 53 (p53), proliferating cell nuclear antigen (P...
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ri-123456789-218112021-12-20T18:29:27Z Immunohistochemical expression of protein 53, murine double minute 2, B-cell lymphoma 2, and proliferating cell nuclear antigen in odontogenic cysts and keratocystic odontogenic tumor Galvão, Hebel Cavalcanti Gordón-Núñez, Manuel Antonio Amorim, Rivadávio Fernandes Batista de Freitas, Roseana de Almeida Souza, Lelia Batista de B-cell lymphoma 2 Dentigerous cyst Keratocystic odontogenic tumor Murine double minute 2 Protein 53 Proliferating cell nuclear antigen Radicular cyst INTRODUCTION: Even though odontogenic cysts share a similar histogenesis, they show different growth and differentiation profile due to differences in the proliferative cellular activity. AIMS: We perform an immunohistochemical assessment of protein 53 (p53), proliferating cell nuclear antigen (PCNA), B-cell lymphoma 2 (bcl-2), and murine double minute 2 (MDM2) expression in odontogenic cysts and keratocystic odontogenic tumor analyzing their correlation with the biological behavior of these lesions. MATERIALS AND METHODS: By the streptavidin-biotin-peroxidase method with antibodies against p53, PCNA, bcl-2, and MDM2 proteins, 11 radicular cysts, 11 dentigerous cysts, and 11 keratocystic odontogenic tumor were analyzed. The non-parametric Mann-Whitney U-test and Kruskall-Wallis test (P ≤ 0.05) were used to analyze the data. RESULTS: Immunopositivity for PCNA was observed in all cases appraised, predominantly in the suprabasal layer of keratocystic odontogenic tumor epithelial lining (SD ± 19.44), but no significant differences were found among the groups of lesions. Bcl-2 immunoexpression was observed especially in the basal layer of keratocystic odontogenic tumor. PCNA LI was significantly higher than bcl-2 LI in keratocystic odontogenic tumor. MDM2 and p53 immunoexpression were not detected in the lesions studied. Among the evaluated lesions, the keratocystic odontogenic tumor showed different immunoexpression of the proliferation and apoptosis markers. CONCLUSION: The results of this study suggest that the keratocystic odontogenic tumor presents distinct biological behavior of the odontogenic cysts, as for the processes of proliferation, apoptosis, and differentiation, reinforcing the information in favor of the neoplastic nature of this lesion. 2017-01-30T14:19:16Z 2017-01-30T14:19:16Z 2013 article GALVÃO, Hébel Cavalcanti et al. Immunohistochemical expression of protein 53, murine double minute 2, B-cell lymphoma 2, and proliferating cell nuclear antigen in odontogenic cysts and keratocystic odontogenic tumor. Indian Journal of Dental Research, v. 24, n. 3, p. 369-374, 2013. https://repositorio.ufrn.br/jspui/handle/123456789/21811 eng Acesso Aberto application/pdf |
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B-cell lymphoma 2 Dentigerous cyst Keratocystic odontogenic tumor Murine double minute 2 Protein 53 Proliferating cell nuclear antigen Radicular cyst |
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B-cell lymphoma 2 Dentigerous cyst Keratocystic odontogenic tumor Murine double minute 2 Protein 53 Proliferating cell nuclear antigen Radicular cyst Galvão, Hebel Cavalcanti Gordón-Núñez, Manuel Antonio Amorim, Rivadávio Fernandes Batista de Freitas, Roseana de Almeida Souza, Lelia Batista de Immunohistochemical expression of protein 53, murine double minute 2, B-cell lymphoma 2, and proliferating cell nuclear antigen in odontogenic cysts and keratocystic odontogenic tumor |
| description |
INTRODUCTION:
Even though odontogenic cysts share a similar histogenesis, they show different growth and differentiation profile due to differences in the proliferative cellular activity.
AIMS:
We perform an immunohistochemical assessment of protein 53 (p53), proliferating cell nuclear antigen (PCNA), B-cell lymphoma 2 (bcl-2), and murine double minute 2 (MDM2) expression in odontogenic cysts and keratocystic odontogenic tumor analyzing their correlation with the biological behavior of these lesions.
MATERIALS AND METHODS:
By the streptavidin-biotin-peroxidase method with antibodies against p53, PCNA, bcl-2, and MDM2 proteins, 11 radicular cysts, 11 dentigerous cysts, and 11 keratocystic odontogenic tumor were analyzed. The non-parametric Mann-Whitney U-test and Kruskall-Wallis test (P ≤ 0.05) were used to analyze the data.
RESULTS:
Immunopositivity for PCNA was observed in all cases appraised, predominantly in the suprabasal layer of keratocystic odontogenic tumor epithelial lining (SD ± 19.44), but no significant differences were found among the groups of lesions. Bcl-2 immunoexpression was observed especially in the basal layer of keratocystic odontogenic tumor. PCNA LI was significantly higher than bcl-2 LI in keratocystic odontogenic tumor. MDM2 and p53 immunoexpression were not detected in the lesions studied. Among the evaluated lesions, the keratocystic odontogenic tumor showed different immunoexpression of the proliferation and apoptosis markers.
CONCLUSION:
The results of this study suggest that the keratocystic odontogenic tumor presents distinct biological behavior of the odontogenic cysts, as for the processes of proliferation, apoptosis, and differentiation, reinforcing the information in favor of the neoplastic nature of this lesion. |
| format |
article |
| author |
Galvão, Hebel Cavalcanti Gordón-Núñez, Manuel Antonio Amorim, Rivadávio Fernandes Batista de Freitas, Roseana de Almeida Souza, Lelia Batista de |
| author_facet |
Galvão, Hebel Cavalcanti Gordón-Núñez, Manuel Antonio Amorim, Rivadávio Fernandes Batista de Freitas, Roseana de Almeida Souza, Lelia Batista de |
| author_sort |
Galvão, Hebel Cavalcanti |
| title |
Immunohistochemical expression of protein 53, murine double minute 2, B-cell lymphoma 2, and proliferating cell nuclear antigen in odontogenic cysts and keratocystic odontogenic tumor |
| title_short |
Immunohistochemical expression of protein 53, murine double minute 2, B-cell lymphoma 2, and proliferating cell nuclear antigen in odontogenic cysts and keratocystic odontogenic tumor |
| title_full |
Immunohistochemical expression of protein 53, murine double minute 2, B-cell lymphoma 2, and proliferating cell nuclear antigen in odontogenic cysts and keratocystic odontogenic tumor |
| title_fullStr |
Immunohistochemical expression of protein 53, murine double minute 2, B-cell lymphoma 2, and proliferating cell nuclear antigen in odontogenic cysts and keratocystic odontogenic tumor |
| title_full_unstemmed |
Immunohistochemical expression of protein 53, murine double minute 2, B-cell lymphoma 2, and proliferating cell nuclear antigen in odontogenic cysts and keratocystic odontogenic tumor |
| title_sort |
immunohistochemical expression of protein 53, murine double minute 2, b-cell lymphoma 2, and proliferating cell nuclear antigen in odontogenic cysts and keratocystic odontogenic tumor |
| publishDate |
2017 |
| url |
https://repositorio.ufrn.br/jspui/handle/123456789/21811 |
| work_keys_str_mv |
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