Análise da imunoexpressão de OCT4 e CD44 em neoplasias de glândulas salivares menores e maiores

Salivary gland neoplasms exhibit a wide variety of biological behavior and a high morphological diversity raises the interest in researching these lesions. The stem cells are the main source for the generation and maintenance of cell diversity, disorders in the regulation of these cells can lead...

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Autor principal: Moura, Jamile Marinho Bezerra de Oliveira
Outros Autores: Souza, Lelia Batista de
Formato: doctoralThesis
Idioma:por
Publicado em: Universidade Federal do Rio Grande do Norte
Assuntos:
Células tronco-tumorais
OCT4
CD44
Imuno-histoquímica
Glândulas salivares
CNPQ::CIENCIAS DA SAUDE: PATOLOGIA ORAL
Endereço do item:https://repositorio.ufrn.br/jspui/handle/123456789/21273
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id ri-123456789-21273
record_format dspace
institution Repositório Institucional
collection RI - UFRN
language por
topic Células tronco-tumorais
OCT4
CD44
Imuno-histoquímica
Glândulas salivares
CNPQ::CIENCIAS DA SAUDE: PATOLOGIA ORAL
spellingShingle Células tronco-tumorais
OCT4
CD44
Imuno-histoquímica
Glândulas salivares
CNPQ::CIENCIAS DA SAUDE: PATOLOGIA ORAL
Moura, Jamile Marinho Bezerra de Oliveira
Análise da imunoexpressão de OCT4 e CD44 em neoplasias de glândulas salivares menores e maiores
description Salivary gland neoplasms exhibit a wide variety of biological behavior and a high morphological diversity raises the interest in researching these lesions. The stem cells are the main source for the generation and maintenance of cell diversity, disorders in the regulation of these cells can lead to the production of altered stem cells, termed cancer stem cells capable of generate the tumor. Researches on cancer stem cells and associated proteins have been developed in some oral cancers; however, their role in salivary gland neoplasms is not well established. Thus, the aim of this study was to identify the tumor parenchyma cells exhibiting stem cell characteristics, by evaluating the immunoreactivity of OCT4 and CD44, in a number of cases of salivary gland neoplasms. The sample consisted of 20 pleomorphic adenomas, 20 mucoepidermoid carcinomas and 20 adenoid cystic carcinoma located in minor and major salivary glands. The expression of OCT4 and CD44 was evaluated by the percentage of positive cells (PP) and the intensity of expression (IE), it is realized the sum of the scores, resulting in the total score immunostaining (PIT) ranging 0-7. All studied cases showed positive expression of OCT4 and CD44 and higher values than the control groups. It was observed that for OCT4 luminal cells and non-luminal were immunostained in the case of pleomorphic adenomas and adenoid cystic carcinoma. Already the immunoreactivity of CD44 was particularly evident in the non-luminal cells of these lesions. In mucoepidermoid carcinomas for both markers, there was immunoreactivity in squamous and intermediate cells and absence of staining mucous cells. For both markers, a statistically significant higher immunostaining was verified in neoplasms located in the major salivary glands compared with lesions in the minor salivary (p<0.001). At the total sample and in the group of minor salivary glands, malignant neoplasms exhibited higher immunoreactivity for OCT4 than pleomorphic adenoma. However, there was no statistically significant difference between the lesions and between their classifications histomorphologic. Analyzing the correlation between OCT4 and CD44 immunoexpressions, a statistically significant moderate positive correlation (r = 0.444) was observed. The high expression of OCT4 and CD44 may indicate that these proteins play an important role in identifying cancer stem cells, allowing a prediction of biological behavior of salivary gland neoplasms.
author2 Souza, Lelia Batista de
author_facet Souza, Lelia Batista de
Moura, Jamile Marinho Bezerra de Oliveira
format doctoralThesis
author Moura, Jamile Marinho Bezerra de Oliveira
author_sort Moura, Jamile Marinho Bezerra de Oliveira
title Análise da imunoexpressão de OCT4 e CD44 em neoplasias de glândulas salivares menores e maiores
title_short Análise da imunoexpressão de OCT4 e CD44 em neoplasias de glândulas salivares menores e maiores
title_full Análise da imunoexpressão de OCT4 e CD44 em neoplasias de glândulas salivares menores e maiores
title_fullStr Análise da imunoexpressão de OCT4 e CD44 em neoplasias de glândulas salivares menores e maiores
title_full_unstemmed Análise da imunoexpressão de OCT4 e CD44 em neoplasias de glândulas salivares menores e maiores
title_sort análise da imunoexpressão de oct4 e cd44 em neoplasias de glândulas salivares menores e maiores
publisher Universidade Federal do Rio Grande do Norte
publishDate 2016
url https://repositorio.ufrn.br/jspui/handle/123456789/21273
work_keys_str_mv AT mourajamilemarinhobezerradeoliveira analisedaimunoexpressaodeoct4ecd44emneoplasiasdeglandulassalivaresmenoresemaiores
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spelling ri-123456789-212732017-11-02T19:12:19Z Análise da imunoexpressão de OCT4 e CD44 em neoplasias de glândulas salivares menores e maiores Moura, Jamile Marinho Bezerra de Oliveira Souza, Lelia Batista de http://lattes.cnpq.br/6671914892609743 Queiroz, Lelia Maria Guedes http://lattes.cnpq.br/3265824235655776 Oliveira, Patricia Teixeira de http://lattes.cnpq.br/4750650943248760 Alves, Pollianna Muniz http://lattes.cnpq.br/4860117599607892 Leitão, Águida Cristina Gomes Henriques http://lattes.cnpq.br/2563401100488039 Células tronco-tumorais OCT4 CD44 Imuno-histoquímica Glândulas salivares CNPQ::CIENCIAS DA SAUDE: PATOLOGIA ORAL Salivary gland neoplasms exhibit a wide variety of biological behavior and a high morphological diversity raises the interest in researching these lesions. The stem cells are the main source for the generation and maintenance of cell diversity, disorders in the regulation of these cells can lead to the production of altered stem cells, termed cancer stem cells capable of generate the tumor. Researches on cancer stem cells and associated proteins have been developed in some oral cancers; however, their role in salivary gland neoplasms is not well established. Thus, the aim of this study was to identify the tumor parenchyma cells exhibiting stem cell characteristics, by evaluating the immunoreactivity of OCT4 and CD44, in a number of cases of salivary gland neoplasms. The sample consisted of 20 pleomorphic adenomas, 20 mucoepidermoid carcinomas and 20 adenoid cystic carcinoma located in minor and major salivary glands. The expression of OCT4 and CD44 was evaluated by the percentage of positive cells (PP) and the intensity of expression (IE), it is realized the sum of the scores, resulting in the total score immunostaining (PIT) ranging 0-7. All studied cases showed positive expression of OCT4 and CD44 and higher values than the control groups. It was observed that for OCT4 luminal cells and non-luminal were immunostained in the case of pleomorphic adenomas and adenoid cystic carcinoma. Already the immunoreactivity of CD44 was particularly evident in the non-luminal cells of these lesions. In mucoepidermoid carcinomas for both markers, there was immunoreactivity in squamous and intermediate cells and absence of staining mucous cells. For both markers, a statistically significant higher immunostaining was verified in neoplasms located in the major salivary glands compared with lesions in the minor salivary (p<0.001). At the total sample and in the group of minor salivary glands, malignant neoplasms exhibited higher immunoreactivity for OCT4 than pleomorphic adenoma. However, there was no statistically significant difference between the lesions and between their classifications histomorphologic. Analyzing the correlation between OCT4 and CD44 immunoexpressions, a statistically significant moderate positive correlation (r = 0.444) was observed. The high expression of OCT4 and CD44 may indicate that these proteins play an important role in identifying cancer stem cells, allowing a prediction of biological behavior of salivary gland neoplasms. Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) As neoplasias de glândulas salivares exibem uma ampla variedade de comportamento biológico e grande diversidade morfológica, e esta heterogeneidade inerente a este grupo de tumores suscita o interesse em pesquisar estas lesões. As células-tronco são a principal fonte para a geração e manutenção da diversidade celular e homeostase do tecido, distúrbios na regulação destas células podem levar à produção de células-tronco alteradas, denominadas de células-tronco tumorais, que possuem potencial proliferativo e capazes de originar e/ou manter o tumor. Pesquisas acerca das células-tronco tumorais e das proteínas a elas associadas em algumas neoplasias orais têm sido desenvolvidas, no entanto, o papel destas em neoplasias de glândulas salivares não está ainda bem estabelecido. Desta forma, o objetivo deste estudo foi identificar células do parênquima tumoral que expressam marcadores de células-tronco tumorais, através da avaliação da imunoexpressão do OCT4 e CD44, em uma série de casos de neoplasias de glândulas salivares. A amostra foi constituída por 20 adenomas pleomórficos, 20 carcinomas mucoepidermóides e 20 carcinomas adenóides císticos localizados nas glândulas salivares menores e maiores. Todos os casos estudados exibiram expressão positiva para OCT4 e CD44, sendo observado que para ambos marcadores, as neoplasias localizadas nas glândulas salivares maiores exibiram maior imunomarcação quando comparada com as lesões das glândulas salivares menores apresentando diferença estatisticamente significativa (p=<0,001). Na amostra total e no grupo das glândulas salivares menores, as neoplasias malignas exibiram maior imunorreatividade para OCT4 do que o adenoma pleomórfico. No entanto, não foi encontrada diferenças estatisticamente significativas de imunoexpressões entre as lesões e entre suas classificações/gradações histomorfológicas. Analisando a correlação entre as imunoexpressões de OCT4 e CD44 foi observada uma correlação positiva moderada (r=0,444) com significância estatística entre os mesmos. A elevada expressão de OCT4 e CD44 pode indicar que estas proteínas desempenham papel importante na identificação de células-tronco tumorais, permitindo uma previsão do comportamento biológico das neoplasias de glândula salivar, apresentando níveis menores em tumores benignos e maiores nos tumores malignos. 2016-08-26T20:02:15Z 2016-08-26T20:02:15Z 2016-02-25 doctoralThesis MOURA, Jamile Marinho Bezerra de Oliveira. Análise da imunoexpressão de OCT4 e CD44 em neoplasias de glândulas salivares menores e maiores. 2016. 137f. Tese (Doutorado em Patologia Oral) - Centro de Ciências da Saúde, Universidade Federal do Rio Grande do Norte, Natal, 2016. https://repositorio.ufrn.br/jspui/handle/123456789/21273 por Acesso Aberto application/pdf Universidade Federal do Rio Grande do Norte Brasil UFRN PROGRAMA DE PÓS-GRADUAÇÃO EM PATOLOGIA ORAL

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