Nanopartículas catiônicas de poli (ácido lático) para liberação modificada de peptídios da peçonha do escorpião Tityus serrulatus
Reported accidents involving the poisoning scorpions are still frequent in Brazil, mainly caused by Tityus serrulatus, known as yellow scorpion. Although antivenom sera are produced routinely by various government laboratories, the effectiveness of its use depends on how quickly treatment is init...
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| Formato: | Dissertação |
| Idioma: | por |
| Publicado em: |
Universidade Federal do Rio Grande do Norte
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| Endereço do item: | https://repositorio.ufrn.br/jspui/handle/123456789/20941 |
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| Resumo: | Reported accidents involving the poisoning scorpions are still frequent in Brazil, mainly
caused by Tityus serrulatus, known as yellow scorpion. Although antivenom sera are
produced routinely by various government laboratories, the effectiveness of its use depends
on how quickly treatment is initiated and efficiency in the production of antibodies by the
immunized animals. In this study, the development of cationic polymeric nanoparticles of
poly(lactic acid) aimed to create a modified delivery system for peptides and proteins of T.
serrulatus venom, able to enhance the production of serum antibodies against the scorpion
toxins. The cationic nanoparticles were obtained by a low energy nanoprecipitation, after
study of the parameters’ variations effects over the physicochemical properties of the
particles. The surface functionalization of the nanoparticles with the hyperbranched
polyethyleneimine was proved by zeta potential analysis and enabled the adsorption by
electrostatic interaction of different types of proteins. The protein loading efficiency of 40-80
% to bovine serum albumin (BSA) and 100 % to scorpion venom peptides evaluated by
spectrophotometry and polyacrylamide gel electrophoresis confirmed the success of the
selected parameters established for obtainment of nanoparticles, produced with size between
100 to 250 nm. The atomic force microscopy analysis and in vitro release showed that the
spherical nanoparticles provided a sustained release profile of proteins by diffusion
mechanism, demonstrating the potential for application of the nanoparticles in vivo. |
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