Immunohistochemical expression of nuclear factor B, matrix metalloproteinase 9, and endoglin (CD105) in odontogenic keratocysts, dentigerous cysts, and radicular cysts

OBJECTIVE: The aim of this study was to compare the immunohistochemical expression of nuclear factor κB (NF-κB), matrix metalloproteinase 9 (MMP-9), and CD105 in odontogenic keratocysts (OKCs), dentigerous cysts (DCs), and radicular cysts (RCs). STUDY DESIGN: Twenty cases of OKCs, 20 DCs, and 20...

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Principais autores:	Santos, Pedro Paulo de Andrade, Aquino, Ana Rafaela Luz de, Barreto, Alessandra Oliveira, Freitas, Roseana de Almeida, Galvão, Hebel Cavalcanti, Souza, Lelia Batista de
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Idioma:	eng
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Assuntos:	Immunohistochemical Matrix Metalloproteinase 9 Odontogenic Cysts
Endereço do item:	https://repositorio.ufrn.br/jspui/handle/123456789/20915
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spelling	ri-123456789-209152021-12-22T17:02:11Z Immunohistochemical expression of nuclear factor B, matrix metalloproteinase 9, and endoglin (CD105) in odontogenic keratocysts, dentigerous cysts, and radicular cysts Santos, Pedro Paulo de Andrade Aquino, Ana Rafaela Luz de Barreto, Alessandra Oliveira Freitas, Roseana de Almeida Galvão, Hebel Cavalcanti Souza, Lelia Batista de Immunohistochemical Matrix Metalloproteinase 9 Odontogenic Cysts OBJECTIVE: The aim of this study was to compare the immunohistochemical expression of nuclear factor κB (NF-κB), matrix metalloproteinase 9 (MMP-9), and CD105 in odontogenic keratocysts (OKCs), dentigerous cysts (DCs), and radicular cysts (RCs). STUDY DESIGN: Twenty cases of OKCs, 20 DCs, and 20 RCs were analyzed. A labeling index (LI), which expresses the percentage of NF-κB-stained nuclei, was calculated for the analysis of NF-κB expression. Expression of MMP-9 in the epithelium and in the capsule of each lesion was scored as 0 (<10% stained cells), 1 (10%-50% stained cells), or 2 (>50% stained cells). In addition, MMP-9 immunostaining was analyzed in endothelial cells of vessels with a conspicuous lumen. The angiogenic index was determined based on the number of anti-CD105 antibody-stained microvessels. RESULTS: In the epithelial component, the NF-κB LI was higher in OKCs than in DCs and RCs (P < .001). Analysis of MMP-9 expression in the epithelial component showed a predominance of score 2 in OKCs (90%), DCs (70%), and RCs (65%; P = .159). Evaluation of the NF-κB LI according to the expression of MMP-9 in the epithelial lining revealed no significant difference between lesions (P = .282). In the fibrous capsule, the highest percentage of MMP-9-stained cells (score 2) was observed in OKCs (P = .100). Analysis of the expression of MMP-9 in the vessels of odontogenic cysts showed a predominance of score 2 in OKCs (80%) and RCs (50%) and of score 1 in DCs (75%; P = .002). Mean microvessel count was high in RCs (16.9), followed by DCs (12.1) and OKCs (10.0; P = .163). No significant difference in microvessel count according to the expression of MMP-9 was observed between groups (P = .689). CONCLUSIONS: The results suggest that the more aggressive biologic behavior of OKCs is related to the higher expression of MMP-9 and NF-κB in those lesions. The differences in the biologic behavior of the lesions studied do not seem to be associated with the angiogenic index. 2016-07-12T15:03:36Z 2016-07-12T15:03:36Z 2011 article SANTOS, Pedro Paulo de Andrade et al. Immunohistochemical expression of nuclear factor ÎºB, matrix metalloproteinase 9, and endoglin (CD10 5) in odontogenic keratocysts, dentigerous cysts, and radicular cysts. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontics , v. 112, n. 4, p. 476-483, 2011. https://repositorio.ufrn.br/jspui/handle/123456789/20915 eng Acesso Aberto application/pdf
institution	Repositório Institucional
collection	RI - UFRN
language	eng
topic	Immunohistochemical Matrix Metalloproteinase 9 Odontogenic Cysts
spellingShingle	Immunohistochemical Matrix Metalloproteinase 9 Odontogenic Cysts Santos, Pedro Paulo de Andrade Aquino, Ana Rafaela Luz de Barreto, Alessandra Oliveira Freitas, Roseana de Almeida Galvão, Hebel Cavalcanti Souza, Lelia Batista de Immunohistochemical expression of nuclear factor B, matrix metalloproteinase 9, and endoglin (CD105) in odontogenic keratocysts, dentigerous cysts, and radicular cysts
description	OBJECTIVE: The aim of this study was to compare the immunohistochemical expression of nuclear factor κB (NF-κB), matrix metalloproteinase 9 (MMP-9), and CD105 in odontogenic keratocysts (OKCs), dentigerous cysts (DCs), and radicular cysts (RCs). STUDY DESIGN: Twenty cases of OKCs, 20 DCs, and 20 RCs were analyzed. A labeling index (LI), which expresses the percentage of NF-κB-stained nuclei, was calculated for the analysis of NF-κB expression. Expression of MMP-9 in the epithelium and in the capsule of each lesion was scored as 0 (<10% stained cells), 1 (10%-50% stained cells), or 2 (>50% stained cells). In addition, MMP-9 immunostaining was analyzed in endothelial cells of vessels with a conspicuous lumen. The angiogenic index was determined based on the number of anti-CD105 antibody-stained microvessels. RESULTS: In the epithelial component, the NF-κB LI was higher in OKCs than in DCs and RCs (P < .001). Analysis of MMP-9 expression in the epithelial component showed a predominance of score 2 in OKCs (90%), DCs (70%), and RCs (65%; P = .159). Evaluation of the NF-κB LI according to the expression of MMP-9 in the epithelial lining revealed no significant difference between lesions (P = .282). In the fibrous capsule, the highest percentage of MMP-9-stained cells (score 2) was observed in OKCs (P = .100). Analysis of the expression of MMP-9 in the vessels of odontogenic cysts showed a predominance of score 2 in OKCs (80%) and RCs (50%) and of score 1 in DCs (75%; P = .002). Mean microvessel count was high in RCs (16.9), followed by DCs (12.1) and OKCs (10.0; P = .163). No significant difference in microvessel count according to the expression of MMP-9 was observed between groups (P = .689). CONCLUSIONS: The results suggest that the more aggressive biologic behavior of OKCs is related to the higher expression of MMP-9 and NF-κB in those lesions. The differences in the biologic behavior of the lesions studied do not seem to be associated with the angiogenic index.
format	article
author	Santos, Pedro Paulo de Andrade Aquino, Ana Rafaela Luz de Barreto, Alessandra Oliveira Freitas, Roseana de Almeida Galvão, Hebel Cavalcanti Souza, Lelia Batista de
author_facet	Santos, Pedro Paulo de Andrade Aquino, Ana Rafaela Luz de Barreto, Alessandra Oliveira Freitas, Roseana de Almeida Galvão, Hebel Cavalcanti Souza, Lelia Batista de
author_sort	Santos, Pedro Paulo de Andrade
title	Immunohistochemical expression of nuclear factor B, matrix metalloproteinase 9, and endoglin (CD105) in odontogenic keratocysts, dentigerous cysts, and radicular cysts
title_short	Immunohistochemical expression of nuclear factor B, matrix metalloproteinase 9, and endoglin (CD105) in odontogenic keratocysts, dentigerous cysts, and radicular cysts
title_full	Immunohistochemical expression of nuclear factor B, matrix metalloproteinase 9, and endoglin (CD105) in odontogenic keratocysts, dentigerous cysts, and radicular cysts
title_fullStr	Immunohistochemical expression of nuclear factor B, matrix metalloproteinase 9, and endoglin (CD105) in odontogenic keratocysts, dentigerous cysts, and radicular cysts
title_full_unstemmed	Immunohistochemical expression of nuclear factor B, matrix metalloproteinase 9, and endoglin (CD105) in odontogenic keratocysts, dentigerous cysts, and radicular cysts
title_sort	immunohistochemical expression of nuclear factor b, matrix metalloproteinase 9, and endoglin (cd105) in odontogenic keratocysts, dentigerous cysts, and radicular cysts
publishDate	2016
url	https://repositorio.ufrn.br/jspui/handle/123456789/20915
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Immunohistochemical expression of nuclear factor B, matrix metalloproteinase 9, and endoglin (CD105) in odontogenic keratocysts, dentigerous cysts, and radicular cysts

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